Transcriptomic Analysis Reveals Regulatory Networks for Osmotic Water Stress and Rewatering Response in the Leaves of Ginkgo biloba
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and PEG Treatment
2.2. RNA Extraction, cDNA Library Preparation, and Sequencing
2.3. Sequence Analysis
2.4. Annotation, Functional Categorization, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes Analysis
2.5. Weighted Gene Coexpression Network Analysis
2.6. Validation of RNA Sequencing Data
3. Results
3.1. Transcriptome Changes in Response to Osmotically Driven Water Stress and Rewatering
3.2. WGCNA Network in Ginkgo Leaves under Osmotic Water Stress and Rewatering
3.3. Analysis of Genes Associated with Flavonoid and Bilobalide Biosynthesis in Ginkgo Leaves under Water Stress and Rewatering
4. Discussion
4.1. DEGs Related to Stress, Development, Transport, Cell Wall, and Hormone Metabolism Might Play Pivotal Roles in Response to Simulated Water Deficit Stress and Rewatering in the Leaves of G. biloba
4.2. Gene Coexpression Networks Probably Contribute to the Response to Osmotic Water Stress and Rewatering in Ginkgo Leaves
4.3. The Expression of Genes That Participated in the Biosynthesis of Flavonoids and Bilobalide Are Induced by Water Stress but Reduced by Rehydration in Ginkgo Leaves
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yu, W.; Cai, J.; Liu, H.; Lu, Z.; Hu, J.; Lu, Y. Transcriptomic Analysis Reveals Regulatory Networks for Osmotic Water Stress and Rewatering Response in the Leaves of Ginkgo biloba. Forests 2021, 12, 1705. https://doi.org/10.3390/f12121705
Yu W, Cai J, Liu H, Lu Z, Hu J, Lu Y. Transcriptomic Analysis Reveals Regulatory Networks for Osmotic Water Stress and Rewatering Response in the Leaves of Ginkgo biloba. Forests. 2021; 12(12):1705. https://doi.org/10.3390/f12121705
Chicago/Turabian StyleYu, Wanwen, Jinfeng Cai, Huimin Liu, Zhiguo Lu, Jingjing Hu, and Yan Lu. 2021. "Transcriptomic Analysis Reveals Regulatory Networks for Osmotic Water Stress and Rewatering Response in the Leaves of Ginkgo biloba" Forests 12, no. 12: 1705. https://doi.org/10.3390/f12121705