System Analysis of MIRNAs in Maize Internode Elongation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material, Growth Conditions, and Phenotypic Evaluation
2.2. RNA Sequencing
2.3. Differential Gene Expression Analysis of miRNAs and mRNA
2.4. Target Gene Prediction and Identification
2.5. Function Enrichment Analysis
2.6. Construction of miRNA–mRNA and PPI Networks
3. Results
3.1. Development of Internodes
3.2. Identification of miRNAs Associated with Internode Elongation
3.3. Comprehensive Analysis for Predictive Target Genes of miRNA
3.4. Identification and Analysis for Target Genes of Internode Elongation Related miRNA
3.5. Analysis of the miRNA–mRNA Network and Construction of PPI
4. Discussion
4.1. miRNAs Involved in Maize Internode Elongation
4.2. miRNAs Affect Cell Expansion and Cell Wall Synthesis by Regulating Their Targets in Maize
4.3. miRNAs Targeting Transcription and Regulatory Factors Are Involved in Internode Elongation
5. Conclusions and Prospects
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Peng, C.; Wang, X.; Feng, T.; He, R.; Zhang, M.; Li, Z.; Zhou, Y.; Duan, L. System Analysis of MIRNAs in Maize Internode Elongation. Biomolecules 2019, 9, 417. https://doi.org/10.3390/biom9090417
Peng C, Wang X, Feng T, He R, Zhang M, Li Z, Zhou Y, Duan L. System Analysis of MIRNAs in Maize Internode Elongation. Biomolecules. 2019; 9(9):417. https://doi.org/10.3390/biom9090417
Chicago/Turabian StylePeng, Chuanxi, Xing Wang, Tianyu Feng, Rui He, Mingcai Zhang, Zhaohu Li, Yuyi Zhou, and Liusheng Duan. 2019. "System Analysis of MIRNAs in Maize Internode Elongation" Biomolecules 9, no. 9: 417. https://doi.org/10.3390/biom9090417