Next Article in Journal
Point Prevalence Survey of Antimicrobial Utilization in Ghana’s Premier Hospital: Implications for Antimicrobial Stewardship
Next Article in Special Issue
Resistance to Ceftazidime/Avibactam, Meropenem/Vaborbactam and Imipenem/Relebactam in Gram-Negative MDR Bacilli: Molecular Mechanisms and Susceptibility Testing
Previous Article in Journal
Using Lactobacilli to Fight Escherichia coli and Staphylococcus aureus Biofilms on Urinary Tract Devices
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Antibiotics
Volume 10
Issue 12
10.3390/antibiotics10121527
antibiotics-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The Spread of NDM-1 and NDM-7-Producing Klebsiella pneumoniae Is Driven by Multiclonal Expansion of High-Risk Clones in Healthcare Institutions in the State of Pará, Brazilian Amazon Region

by
Yan Corrêa Rodrigues
,
Amália Raiana Fonseca Lobato
,
Ana Judith Pires Garcia Quaresma
,
Lívia Maria Guimarães Dutra Guerra
and
Danielle Murici Brasiliense
*
Bacteriology and Mycology Section, Evandro Chagas Institute (SABMI/IEC), Ananindeua 67030-000, PA, Brazil
*
Author to whom correspondence should be addressed.
Antibiotics 2021, 10(12), 1527; https://doi.org/10.3390/antibiotics10121527
Submission received: 26 October 2021 / Revised: 11 November 2021 / Accepted: 17 November 2021 / Published: 14 December 2021
(This article belongs to the Special Issue Multi-Drug Resistant Gram-Negative Infections: Molecular Epidemiology, Microbiological Diagnosis and Antimicrobial Treatment)
Browse Figure
Versions Notes

Abstract

:
Carbapenem resistance among Klebsiella pneumoniae isolates is often related to carbapenemase genes, located in genetic transmissible elements, particularly the blaKPC gene, which variants are spread in several countries. Recently, reports of K. pneumoniae isolates harboring the blaNDM gene have increased dramatically along with the dissemination of epidemic high-risk clones (HRCs). In the present study, we report the multiclonal spread of New Delhi metallo-beta-lactamase (NDM)-producing K. pneumoniae in different healthcare institutions in the state of Pará, Northern Brazil. A total of 23 NDM-producing isolates were tested regarding antimicrobial susceptibility testing features, screening of carbapenemase genes, and genotyping by multilocus sequencing typing (MLST). All K. pneumoniae isolates were determined as multidrug-resistant (MDR), being mainly resistant to carbapenems, cephalosporins, and fluoroquinolones. The blaNDM-7 (60.9%—14/23) and blaNDM-1 (34.8%—8/23) variants were detected. MLST genotyping revealed the predomination of HRCs, including ST11/CC258, ST340/CC258, ST15/CC15, ST392/CC147, among others. To conclude, the present study reveals the contribution of HRCs and non-HRCs in the spread of NDM-1 and NDM-7-producing K. pneumoniae isolates in Northern (Amazon region) Brazil, along with the first detection of NDM-7 variant in Latin America and Brazil, highlighting the need for surveillance and control of strains that may negatively impact healthcare and antimicrobial resistance.

1. Introduction

Klebsiella pneumoniae is among the major pathogens causing healthcare-related infections (HAIs) and outbreaks in several healthcare institutions. This is due to its antimicrobial resistance (AMR) and virulence traits, leading to the increasingly frequent reports of severe infections, poor prognosis outcomes, and limitation of antimicrobial therapy options [1,2].
The aggravating problem of AMR among K. pneumoniae isolates is commonly related to the spread of plasmid-borne resistance genes, including metallo-beta-lactamases (MβLs) and extended spectrum beta-lactamases (ESβLs). Carbapenem-resistant K. pneumoniae isolates (CR-Kp) are usually detected harboring the blaKPC gene, with endemicity reported in Brazil and several other countries, while the dissemination of NDM-producing K. pneumoniae has increased in several regions, and mainly during the COVID-19 pandemic [3,4,5,6,7,8]. Such highly resistant-strains carrying resistance mechanisms are associated with so-called high-risk clones (HRCs), particularly to strains belonging to the clonal complex 258 (CC258) and CC15, which have been detected in multiclonal expansion at several Brazilian hospitals [9,10,11,12,13,14].
Even though other studies have demonstrated the spread of blaKPC and blaNDM by K. pneumoniae HRC strains across Brazil [12,15,16,17,18], critical knowledge gaps remain regarding this distribution and genetic background contributing to this dissemination in Northern Brazil. Herein, we report a genetic background mostly composed of HRCs, and the multiclonal spread of NDM-1 and NDM-7-producing K. pneumoniae in different healthcare institutions in the state of Pará, the Brazilian Amazon region.

2. Results

2.1. Bacterial Isolates and Susceptibility Characteristics

K. pneumoniae isolates were recovered from patients at nine different healthcare institutions in the region (H1-H9), of which 43.5% (n = 10) were hospitalized in intensive care unit (ICUs) and 56.5% (n = 13) in clinical wards (non-ICU settings). Clinical specimen of K. pneumoniae samples included: urine (n = 8), blood (n = 3), tracheal secretion (n = 3), rectal swab (n = 3), bronchoalveolar lavage (n = 1), abdominal abscess secretion (n = 1), wound secretion (n = 1), nasopharyngeal secretion (n = 1), soft tissue secretion (n = 1), and peritoneal fluid (n = 1) (Table 1).
Antimicrobial susceptibility testing (ATS) results demonstrated that the majority of K. pneumoniae isolates were non-susceptible to the tested antimicrobial classes and phenotypically classified as multi-drug resistant (MDR—possible XDR) (23/23—100.0%). Isolates exhibited a high frequency of resistance to carbapenems (ETP and MEM 23/23—100.0%; IMP 22/23 95.7%), 3rd/4th generation cephalosporins (CAZ, CRO and FEP 23/23—100.0%), and fluoroquinolones (CIP 21/23—91.3%). Differently, tested isolates were mainly susceptible to TGC (18/23—78.3%) and aminoglycosides (AMK 13/23—56.5%; GEN 12/23—52.2%). The mCIM/eCIM essays revealed that all of the isolates were carbapenemase-producing (mCIM-positive); however, three isolates were negative to EDTA inhibition (eCIM) (Table 2).
Molecular screening of carbapenemase genes showed that all K. pneumoniae isolates harbored the blaNDM gene (23/23—100.0%), including 60.9% (14/23) defined as blaNDM-7 variant and 34.8% (8/23) as a blaNDM-1 variant. The definition of the blaNDM subtype of one isolate (4.3%) could not be performed. Moreover, two isolates were detected (8.7%) co-harboring the blaNDM-7/blaKPC-2 genes, while the other two (8.7%) were the blaNDM-1/blaKPC-2 genes. The blaIMP, blaVIM, and blaOXA-48 genes were not detected (Table 1 and Table 2).

2.2. Molecular Typing by Multilocus Sequencing Typing—MLST

MLST genotyping and genetic relationship analysis revealed a genetic background mostly composed by HRCs, including nine sequence types (STs) associated with four clonal complexes (CCs), including: HRC ST11/CC258 (10/23—43.5%), HRC ST15/CC15 (5/23—21.7%), HRC ST340/CC258 (1/23—4.3%), HRC ST392/CC147 (1/23—4.3%), HRC ST1264/CC258 (1/23—4.3%), ST1401/CC1401(1/23—4.3%) and ST138/CC138 (1/23—4.3%); while ST3449 (1/23—4.3%) and ST3512 (1/23—4.3%) ST4398 (1/23—4.3%) were classified as singletons (Table 1 and Figure 1).
The blaNDM-7 variant was found to spread in seven healthcare institutions (H1, H2, H3, H5, H7, H8, and H9) mostly related to K. pneumoniae CC258 strains, including ST11 and ST1264, and non-HRCs. Oppositely, the dissemination of the blaNDM-1 variant was related to three STs (ST15, ST11, and ST340) at four hospitals (H2, H3, H4, and H6). Finally, the four isolates harboring both blaNDM and blaKPC genes were associated with the HRC ST11/CC258 circulating at H3 and H6 (Table 1).

3. Discussion

The emergence and spread of MDR CR-Kp strains have been reported worldwide and in Brazil, causing a critical impact on the increasing levels of antimicrobial resistance and leading to a public health crisis due to the limitation of antimicrobial therapy options, infection severity, and challenging spread control. Such strains are usually related to epidemic HRCs harboring plasmid-borne carbapenemases, particularly the blaKPC, and recently blaNDM, emphasizing the importance of epidemiological vigilance and recognizing medically relevant resistant features strains. The present study reports the spread of NDM-producing K. pneumoniae strains associated with HRCs in different healthcare institutions in the state of Pará, Brazilian Amazon region.
The resistance trend for 3rd/4th generation cephalosporins and carbapenems among K. pneumoniae isolates have been reported in several countries, and in Latin America, countries including Brazil, Argentina and Chile account for most of the cases [1,2,5]. Indeed, on recently reported data of national surveillance on antimicrobial resistance, K. pneumoniae isolated in adult and pediatric Brazilian ICUs presented resistance rates ranging 49.3% to 68.1% and 19.3% to 51.8% to 3rd/4th generation cephalosporins and carbapenems, respectively [19]. Previous reports suggest the increasing prevalence of MDR CR-Kp isolates in the Brazilian Amazon region [20,21,22]. As in line with the discussed data, the antimicrobial susceptibility features of K. pneumoniae isolates in our study demonstrated that all tested isolates were MDR (possible XDR), predominantly exhibiting combined resistance to 3rd/4th generation cephalosporins, carbapenems, and fluoroquinolones. Furthermore, even though the transmission of MDR K. pneumoniae between patients is an important mechanism for outbreaks occurrence, especially in ICUs [23,24], the presence of highly resistant isolates in non-ICU settings (13/23—56.5%) was observed. This points out the spread of these strains in different wards and/or different healthcare facilities, which may also be associated and contribute to disseminating antibiotic resistance markers, such as blaNDM and blaKPC.
CR-Kp has been typically related to transmissible genetic elements added to carbapenemases genes, such as blaNDM and blaKPC, which have been increasingly detected in different countries, emphasizing their worldwide dissemination [7,8,25,26]. In Brazil, KPC has become endemic and widely disseminated among K. pneumoniae from hospitals in all Brazilian regions [5,14,17,20,21,27,28,29,30]. Since 2013, NDM-producing isolates have been reported in different Brazilian regions and associated with a wide variety of bacterial species, including Escherichia coli, Acinetobacter baumannii, A. nosocomialis, A. pittii, Enterobacter cloacae, Enterobacter hormaechei, and K. pneumoniae [12,15,16,31,32,33,34,35]. Recently, Silva et al. [16], in a study evaluating 81 bacterial isolates from different regions, demonstrated that K. pneumoniae is responsible for the widespread of this carbapenemase in Brazilian hospitals, confirming more efficient dissemination compared with the blaKPC variant. The study, however, was limited by not including isolates from the Brazilian Amazon region, evidencing the absence of data on NDM-producing K. pneumoniae isolates in the region. Interestingly, the present study revealed 23 CR-Kp isolates were harboring blaNDM gene in nine different hospitals in the same region. This elevated frequency of NDM-producing CR-Kp has been only reported by Vivas et al. [15] in Sergipe (Northeast Brazil), where among the 147 investigated isolates, over 50.0% were blaNDM carriers.
The NDM-7 variant was firstly described in E. coli and presented in its structure two amino acid substitutions compared to NDM-1 [36,37]. This variant has been related to infection by MDR microorganisms, predominantly in Asian countries, such as India, Japan, and China [38,39,40]. Despite being mainly associated with E. coli and K. pneumoniae isolates, NDM-7 has already been described in other members of Enterobacterales, reinforcing its ability to spread among different bacterial genera [41]. Furthermore, some studies have suggested more enzymatic hydrolysis activity against carbapenem, comparing NDM-7 to NDM-1 [42]. This study describes the alarming dissemination of NDM -1 and NDM-7 in association with K. pneumoniae HRCs in different healthcare institutions in northern Brazil, being the first detection of NDM-7 circulating in Latin America and Brazil.
Worryingly, the co-production and accumulation of genetic resistance determinants have become typically reported among highly resistant K. pneumoniae isolates. Certainly, most of the NDM-producing isolates co-harbor a broad variety of resistance mechanisms, such as CTX-M, SHV, KPC, VIM, and OXA-48, highlighting the increasing incidence of blaNDM and blaKPC co-producing strains, as reported in China, USA, Greece, India, Pakistan and Turkey [7,25,43,44,45,46,47,48]. In April 2021, Argentina’s National Antimicrobial Reference Laboratory was alerted to the emergence and spread of Enterobacterales producing different combinations of carbapenemases, especially during the first wave of the COVID-19 pandemic. Approximately one-third of the isolates received at this center (27.0%—52/196) were co-producers, with a combination of serine and MβLs, of which 60% had a combination of KPC and NDM [49]. In Brazil, the presence of over 20 blaNDM and blaKPC K. pneumoniae co-producing isolates, distributed among the Northeast, Southeast and South regions, have been described [12,15,27,31,50]. In the present study, four CR-Kp isolates were detected co-harboring blaNDM and blaKPC. Three were recovered from patients hospitalized in the pediatric ICU at the same hospital (H3), suggesting that the persistence of high resistance levels strains in the environment and urgent strengthening of surveillance measures.
Investigations on the molecular epidemiology of K. pneumoniae isolates demonstrated that a small subset of successful HRCs is responsible for undermining antimicrobial therapy options, severe and poor prognosis infections, and nosocomial outbreaks globally. K. pneumoniae HCRs exhibit a high resistance degree, including resistance to 3rd/4th generation cephalosporins and carbapenems, and are remarkably effective reservoirs and vehicles for disseminating genetic resistance mechanisms, such as ESBLs and carbapenemases [7,10,51]. Molecular epidemiology analysis based on MLST revealed an absence of relationship among most of the evaluated K. pneumoniae strains, including the presence of nine distinct STs belonging to four CCs and predominance of MDR HRCs, which corroborates the hypothesis of epidemic multiclonal expansion of K. pneumoniae in Brazilian hospitals and globally.
Carbapenemase-producing clones are mostly associated with CC258, including STs 11, 101, 258, 340, 437, 512, 874, and 1264. ST11 and ST340/CC258 are globally distributed and have been detected harboring carbapenemases regardless of their type, and in Brazil, they are mostly associated with KPC-producing strains in almost all Brazilian regions [7,13,14,17,52,53,54,55]. ST1264 has emerged (and probably highly restricted) in China, causing bloodstream infection, and recently associated with ESBL-producing isolates [56,57]. Recently, several reports indicate ST340/CC258 has a critical role in expanding NDM-producing strains in Brazil, being associated with monoclonal outbreaks, colistin-resistant-isolates, and isolates co-harboring blaNDM and blaKPC genes [9,12,18,58]. Epidemic ST11 was the most prevalent in our study, found dispersed in five distinct hospitals in different periods, and interestingly present in pediatric settings at H3, co-harboring blaNDM, and blaKPC, indicating endemicity and spread these resistance markers in this environment. ST340 was related to a single isolate recovered from a patient in the adult ICU at H3. To the best of our knowledge, the present study first provides data on the dissemination of NDM and NDM/KPC-producing K. pneumoniae associated with ST11/CC258 among clinical isolates in Brazil and corroborates the dissemination of ST340 NDM-producing strains in Brazil. Finally, our results also contribute to a better comprehension of the epidemiology of clinically important carbapenem resistance markers in the Brazilian territory and globally.
The HRC ST15/CC15 is usually related to CR-Kp carrying plasmid-borne MβLs in several locations in Europe, India, Nepal, Pakistan, and China, indicating a high capacity for the horizontal acquisition of resistance genes [6,10,59,60]. In Latin America, ST15/CC15 has been less frequently described than ST11 and ST340. However, it has demonstrated a concerning spread, with strains particularly harboring the blaNDM gene and rarely associated with blaKPC [54,61]. Since its first report in Brazil by Gonçalves et al. [17], ST15/CC15 has been detected in clinical and environmental samples, but with few reported NDM-producing strains in Porto Alegre (South region), Rio de Janeiro (Southeast), and Brasilia (Midwest region) cities [9,50,54,62]. According to our data, NDM-producing ST15/CC15 strains have been found circulating in four different hospitals since 2018 and mostly present adult settings, suggesting an early and rapid spread across Brazil.
K. pneumoniae ST392/CC147 has been reported related to nosocomial infection in various countries and described as an emerging clinically important HRC. Its endemicity has been related to the clonal spread of KPC-3-producing strains in Italy, VIM-producing strains in Grecia, while in Colombia, Mexico, and Iran with NDM-producing strains [63,64,65,66,67]. In Brazil, few reports revealed strains co-harboring blaKPC/mcr-1 and blaKPC/blaOXA in Espírito Santo (Southern Brazil) and Tocantins (Northern Brazil), respectively [12,21]. In the present study, ST392 was detected in the pediatric clinic at H3, and, as far as we know, this is the first report of NDM-producing K. pneumoniae belonging to ST392/CC147 in Brazil.
Despite the non-HRCs being found related with the minority of NDM-producing K. pneumoniae isolates in our study, they were interestingly detected carrying the blaNDM-7 variant. ST1401 was first described in a human blood sample from Kuwait and has been reported in the USA and Mexico [68,69,70]. ST138/CC138 has been associated with KPC-2-producing and NDM-7-producing isolates in Brazil and Canada, respectively [14,71]. ST3449 was detected in China in an isolate harboring the blaNDM gene [72]. An NDM/KPC-producing K. quasipneumoniae isolate belonging to ST3512 was to be causing bloodstream infection in Bahia State (Northeast Brazil) [11]. Wyres et al. [10] highlight that non-HCR may only cause localized problems; however, their emergence, spread, and persistence as HRC are influenced by several factors, which are mostly unknown. In this sense, our results draw attention to the importance of non-HRCs in the spread of NDM-7-producing strains at four hospitals (H1, H2, H7, and H8) in the Brazilian Amazon region, also been, to the best of our knowledge, the first report of ST1401, ST138, ST3449 and ST4398 carrying the blaNDM-7 gene in Latin America and Brazil.

4. Materials and Methods

4.1. Bacterial Isolates and Species Identification

The present cross-sectional study included 23 non-duplicated K. pneumoniae isolates stored at the Bacteriology and Mycology Section, Evandro Chagas Institute, a referral surveillance center located in the State of Pará, Brazilian Amazon region. From January 2018 to February 2021, K. pneumoniae isolates were obtained from several clinical sources of non-consecutive patients admitted at nine different hospitals in the region (H1-H9). Bacterial suspensions for each sample were prepared to match the 0.5 McFarland standard, followed by isolates identification on the automated VITEK-2 system (bioMérieux, Marcy l’Etoile, France) using the VITEK-2 card GN Test Kit Ref: 21341 for Gram-negative species identification.

4.2. Antimicrobial Susceptibility-Related Assays

AST was performed by broth microdilution on the automated VITEK-2 system (bioMérieux, Marcy l’Etoile, France) for 10 antimicrobial categories, including penicillins (ampicillin—AMP), penicillins + β-lactamase inhibitors (ampicillin-sulbactam—SAM), antipseudomonal penicillins + β-lactamase inhibitors (piperacillin + tazobactam—TZP), non-extended spectrum cephalosporins/2nd generation cephalosporins (cefuroxime—CXM), cephamycins (cefoxitin—FOX), extended-spectrum cephalosporins/3rd and 4th generation cephalosporins (ceftazidime—CAZ; ceftriaxone—CRO and cefepime—FEP), carbapenems (imipenem—IMP; meropenem—MEM and ertapenem—ETP), aminoglycosides (amikacin—AMK and gentamicin—GEN), fluoroquinolones (ciprofloxacin—CIP) and glycylcyclines (tigecycline—TGC). ATS was conducted following the manufacturer’s requirements, and as a result, isolates were classified as sensitive (S), intermediate (I), and resistant (R), based on breakpoints by the Clinical and Laboratory Standards Institute (CLSI), except for TGC, which was considered the FDA criteria [73,74].
Additionally, isolates were phenotypically categorized as MDR if resistant to ≥1 drug in ≥3 antimicrobial categories, according to criteria proposed by Magiorakos et al. [75]. Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 were used as quality control strains. Finally, all isolates exhibiting non-susceptibility to carbapenems (IMP, MER and/or ETP) were phenotypically tested for the presence of carbapenemase using the test of inactivation of carbapenem (mCIM/eCIM), following the CLSI recommendations [73].

4.3. Molecular Screening of β-Lactamase-Encoding Genes

Bacterial genomic DNA was extracted from overnight cultures, where a single K. pneumoniae colony was suspended in 300μL of distilled water and boiled at 95 °C for 10 min, followed by incubation at −20 °C for 15 min, and final centrifugation at 12,000 rpm for 7 min. The obtained DNA samples were stored at −20 °C until testing and used for all molecular assays. The molecular detection of β-lactamase-related genes (blaKPC, blaNDM, blaIMP, blaVIM, and blaOXA-48) was performed by PCR using the previously described primers and methodology [76]. PCR products were analyzed under UV light after electrophoresis on 1% agarose gel stained with SYBR™ Safe DNA gel stain (Life Technologies, Carlsbad, CA, USA). Definition of blaNDM and blaKPC subtypes was performed by direct sequencing of purified PCR products using the BigDye™ Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Carlsbad, CA, USA) on an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). This was followed by the analysis of the obtained sequences at the BLAST search, available at the NCBI website (http://blast.ncbi.nih.gov/Blast.cgi (accessed on: 21 October 2021)) [76,77].

4.4. Genetic Diversity Assessment by MLST

Molecular typing by MLST was performed in accordance with a protocol previously described by Diancourt et al. [78], slightly modified by using universal sequencing primers. The seven housekeeping genes included in the scheme (gapA, infB, mdh, pgi, phoE, rpoB, and tonB) were amplified by PCR, followed by sequencing of reaction products using the BigDye™ Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Carlsbad, CA, USA) on an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). Determination of allele profiles and sequence types (STs) was conducted by comparing the obtained sequences to the documented data at Klebsiella PasteurMLST database (https://bigsdb.web.pasteur.fr/Klebsiella/Klebsiella.html (accessed on 21 October 2021). PHYLOViZ 2.0 platform was used for data management and analysis of clonal complexes (CCs), which were defined by related ST clusters exhibiting variation in a single locus (single locus variants—SLV) [79].

5. Conclusions

In conclusion, the present study demonstrates the multiclonal dissemination of MDR K. pneumoniae HRCs producing NDM-1 and NDM-7 carbapenemases in different hospitals in Northern (Amazon region) Brazil. This highlights the need for reinforcement of surveillance and control measures for such strains. Epidemic HRCs ST11/CC258 and ST392/CC147 were firstly associated with NDM-producing strains in Brazil and the first detection of the NDM-7 variant in Latin America and Brazil. Finally, the concerning diversity of NDM variants associated with a diverse genetic background of K. pneumoniae strains suggests an early endemicity for this carbapenemase in the country, which may negatively impact healthcare and antimicrobial resistance scenarios locally and nationally.

Author Contributions

Conceptualization, D.M.B. and Y.C.R.; methodology, D.M.B.; validation, D.M.B., Y.C.R. and A.J.P.G.Q.; investigation, Y.C.R., A.R.F.L., A.J.P.G.Q. and L.M.G.D.G.; resources, D.M.B.; data curation, D.M.B., Y.C.R. and A.R.F.L.; writing—original draft preparation, D.M.B. and Y.C.R.; writing—review and editing, D.M.B. and Y.C.R.; visualization, D.M.B., Y.C.R. and A.R.F.L.; supervision, D.M.B.; project administration, D.M.B.; funding acquisition, D.M.B. All authors have read and agreed to the published version of the manuscript.

Funding

The present study and the APC were funded and supported by Evandro Chagas Institute, Secretariat of Health Surveillance, and Brazilian Ministry of Health.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

All relevant data is presented within the manuscript.

Acknowledgments

The authors thank the technical support of Michelle Ribeiro and Raimundo Nonato, as well as the health institutions that sent the bacterial isolates used in the present study. We also thank Anderson Fabiano Albuquerque Silva for his assistance in preparing and editing the MST figure.

Conflicts of Interest

The authors declare no conflict of interest regarding the publication of the present study.

References

  1. Campos, J.C.D.M.; Antunes, L.C.M.; Ferreira, R.B.R. Global Priority Pathogens: Virulence, Antimicrobial Resistance and Prospective Treatment Options. Future Microbiol. 2020, 15, 649–677. [Google Scholar] [CrossRef]
  2. World Health Organization. Global Antimicrobial Resistance and Use Surveillance System (GLASS) Report; Agnew, E., Dolecek, C., Hasan, R., Lahra, M., Merk, H., Perovic, O., Sievert, D., Smith, R., Taylor, A., Turner, P., Eds.; World Health Organization: Geneva, Switzerland, 2021. [Google Scholar]
  3. Amarsy, R.; Jacquier, H.; Munier, A.L.; Merimèche, M.; Berçot, B.; Mégarbane, B. Outbreak of NDM-1-Producing Klebsiella pneumoniae in the Intensive Care Unit during the COVID-19 Pandemic: Another Nightmare. Am. J. Infect. Control 2021, 49, 1324–1326. [Google Scholar] [CrossRef] [PubMed]
  4. Nori, P.; Szymczak, W.; Puius, Y.; Sharma, A.; Cowman, K.; Gialanella, P.; Fleischner, Z.; Corpuz, M.; Torres-Isasiga, J.; Bartash, R.; et al. Emerging Co-Pathogens: New Delhi Metallo-beta-lactamase producing Enterobacterales Infections in New York City COVID-19 Patients. Int. J. Antimicrob. Agents 2020, 56, 106179. [Google Scholar] [CrossRef]
  5. García-Betancur, J.C.; Appel, T.M.; Esparza, G.; Gales, A.C.; Levy-Hara, G.; Cornistein, W.; Vega, S.; Nuñez, D.; Cuellar, L.; Bavestrello, L.; et al. Update on the Epidemiology of Carbapenemases in Latin America and the Caribbean. Expert Rev. Anti-Infect. Ther. 2021, 19, 197–213. [Google Scholar] [CrossRef]
  6. David, S.; Reuter, S.; Harris, S.R.; Glasner, C.; Feltwell, T.; Argimon, S.; Abudahab, K.; Goater, R.; Giani, T.; Errico, G.; et al. Epidemic of Carbapenem-Resistant Klebsiella pneumoniae in Europe Is Driven by Nosocomial Spread. Nat. Microbiol. 2019, 4, 1919–1929. [Google Scholar] [CrossRef]
  7. Lee, C.R.; Lee, J.H.; Park, K.S.; Kim, Y.B.; Jeong, B.C.; Lee, S.H. Global Dissemination of Carbapenemase-Producing Klebsiella Pneumoniae: Epidemiology, Genetic Context, Treatment Options, and Detection Methods. Front. Microbiol. 2016, 7, 1–30. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  8. Sampaio, J.L.M.; Gales, A.C. Antimicrobial Resistance in Enterobacteriaceae in Brazil: Focus on β-Lactams and Polymyxins. Braz. J. Microbiol. 2016, 47, 31–37. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  9. Lee, A.H.Y.; Porto, W.F.; de Faria Jr, C.; Dias, S.C.; Alencar, S.A.; Pickard, D.J.; Hancock, R.E.W.; Franco, O.L. Genomic Insights into the Diversity, Virulence and Resistance of Klebsiella pneumoniae Extensively Drug Resistant Clinical Isolates. Microb. Genom. 2021, 7, 000613. [Google Scholar] [CrossRef] [PubMed]
  10. Wyres, K.L.; Lam, M.M.C.; Holt, K.E. Population Genomics of Klebsiella Pneumoniae. Nat. Rev. Microbiol. 2020, 18, 344–359. [Google Scholar] [CrossRef]
  11. Silveira, M.C.; Rocha-de-Souza, C.M.; de Oliveira Santos, I.C.; Pontes, L.d.S.; Oliveira, T.R.T.e.; Tavares-Teixeira, C.B.; Cossatis, N.d.A.; Pereira, N.F.; da Conceição-Neto, O.C.; da Costa, B.S.; et al. Genetic Basis of Antimicrobial Resistant Gram-Negative Bacteria Isolated From Bloodstream in Brazil. Front. Med. 2021, 8, 1–9. [Google Scholar] [CrossRef]
  12. Aires, C.A.M.; Araujo, C.F.M.d.; Chagas, T.P.G.; Oliveira, J.C.R.; Buonora, S.N.; Albano, R.M.; Carvalho-Assef, A.P.D.; Asensia, M.D. Multiclonal Expansion of Klebsiella pneumoniae Isolates Producing NDM-1 in Rio de Janeiro, Brazil. Antimicrob. Agents Chemother. 2017, 61, e01048-16. [Google Scholar] [CrossRef] [Green Version]
  13. Bowers, J.R.; Kitchel, B.; Driebe, E.M.; MacCannell, D.R.; Roe, C.; Lemmer, D.; De Man, T.; Rasheed, J.K.; Engelthaler, D.M.; Keim, P.; et al. Genomic Analysis of the Emergence and Rapid Global Dissemination of the Clonal Group 258 Klebsiella pneumoniae Pandemic. PLoS ONE 2015, 10, e133727. [Google Scholar] [CrossRef] [Green Version]
  14. Pereira, P.S.; De araujo, C.F.M.; Seki, L.M.; Zahner, V.; Carvalho-Assef, A.P.D.A.; Asensi, M.D. Update of the Molecular Epidemiology of KPC-2-Producing Klebsiella pneumoniae in Brazil: Spread of Clonal Complex 11 (ST11, ST437 and ST340). J. Antimicrob. Chemother. 2013, 68, 312–316. [Google Scholar] [CrossRef] [PubMed]
  15. Vivas, R.; Dolabella, S.S.; Barbosa, A.A.T.; Jain, S. Prevalence of Klebsiella pneumoniae Carbapenemase-and New Delhi Metallo-Beta-Lactamase-Positive k. Pneumoniae in Sergipe, Brazil, and Combination Therapy as a Potential Treatment Option. Rev. Soc. Bras. Med. Trop. 2020, 53, 1–8. [Google Scholar] [CrossRef] [PubMed]
  16. Da Silva, I.R.; Aires, C.A.M.; Conceição-Neto, O.C.; De Oliveira Santos, I.C.; Ferreira Pereira, N.; Moreno Senna, J.P.; Carvalho-Assef, A.P.D.A.; Asensi, M.D.; Rocha-De-Souza, C.M. Distribution of Clinical NDM-1-Producing Gram-Negative Bacteria in Brazil. Microb. Drug Resist. 2019, 25, 394–399. [Google Scholar] [CrossRef] [PubMed]
  17. Gonçalves, G.B.; Furlan, J.P.R.; Vespero, E.C.; Pelisson, M.; Stehling, E.G.; Pitondo-Silva, A. Spread of Multidrug-Resistant High-Risk Klebsiella pneumoniae Clones in a Tertiary Hospital from Southern Brazil. Infect. Genet. Evol. 2017, 56, 1–7. [Google Scholar] [CrossRef] [PubMed]
  18. Boszczowski, I.; Salomão, M.C.; Moura, M.L.; Freire, M.P.; Guimarães, T.; Cury, A.P.; Rossi, F.; Rizek, C.F.; Ruedas Martins, R.C.; Costa, S.F. Multidrug-Resistant Klebsiella Pneumoniae: Genetic Diversity, Mechanisms of Resistance to Polymyxins and Clinical Outcomes in a Tertiary Teaching Hospital in Brazil. Rev. Inst. Med. Trop. Sao Paulo 2019, 61, e29. [Google Scholar] [CrossRef] [PubMed]
  19. Agencia Nacional de Vigilância Sanitária. Boletim Segurança do Paciente e Qualidade em Serviços de Saúde nº 22-Avaliação Nacional dos Indicadores de IRAS e RM-2019; ANVISA: Brasilia, Brazil, 2020; p. 6. Available online: https://app.powerbi.com/view?r=eyJrIjoiZjQ5ZDhjZmEtNDdhOC00MDk3LWFiNDEtNzg0MmE4MmE2MjlhIiwidCI6ImI2N2FmMjNmLWMzZjMtNGQzNS04MGM3LWI3MDg1ZjVlZGQ4MSJ9&pageName=ReportSectionac5c0437dbe709793b4b (accessed on 21 October 2021).
  20. Dos Santos, A.L.S.; Rodrigues, Y.C.; de Melo, M.V.H.; dos Santos, P.A.S.; da Costa Oliveira, T.N.; Sardinha, D.M.; Lima, L.N.G.C.; Brasiliense, D.M.; Lima, K.V.B. First Insights into Clinical and Resistance Features of Infections by Klebsiella pneumoniae among Oncological Patients from a Referral Center in Amazon Region, Brazil. Infect. Dis. Rep. 2020, 12, 110–120. [Google Scholar] [CrossRef] [PubMed]
  21. Ferreira, R.L.; Da Silva, B.C.M.; Rezende, G.S.; Nakamura-Silva, R.; Pitondo-Silva, A.; Campanini, E.B.; Brito, M.C.A.; Da Silva, E.M.L.; De Melo Freire, C.C.; Da Cunha, A.F.; et al. High Prevalence of Multidrug-Resistant Klebsiella pneumoniae Harboring Several Virulence and β-Lactamase Encoding Genes in a Brazilian Intensive Care Unit. Front. Microbiol. 2019, 9, 3198. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  22. Ferreira, C.M. Molecular Epidemiology of KPC-2 Producing Klebsiella Pneumoniae. SOJ Microbiol. Infect. Dis. 2017, 5, 1–3. [Google Scholar] [CrossRef]
  23. Theuretzbacher, U. Global Antimicrobial Resistance in Gram-Negative Pathogens and Clinical Need. Curr. Opin. Microbiol. 2017, 39, 106–112. [Google Scholar] [CrossRef] [PubMed]
  24. Haque, M.; Sartelli, M.; Mckimm, J.; Abu Bakar, M. Infection and Drug Resistance Dovepress Health Care-Associated Infections-an Overview. Infect. Drug Resist. 2018, 11, 2321–2333. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  25. Liu, X.; Zhang, J.; Li, Y.; Shen, Q.; Jiang, W.; Zhao, K.; He, Y.; Dai, P.; Nie, Z.; Xu, X.; et al. Diversity and Frequency of Resistance and Virulence Genes in BlaKPC and BlaNDM Co-Producing Klebsiella pneumoniae Strains from China. Infect. Drug Resist. 2019, 12, 2819–2826. [Google Scholar] [CrossRef] [Green Version]
  26. Munoz-Price, L.S.; Poirel, L.; Bonomo, R.A.; Schwaber, M.J.; Daikos, G.L.; Cormican, M.; Cornaglia, G.; Garau, J.; Gniadkowski, M.; Hayden, M.K.; et al. Clinical Epidemiology of the Global Expansion of Klebsiella pneumoniae Carbapenemases. Lancet Infect. Dis. 2013, 13, 785–796. [Google Scholar] [CrossRef] [Green Version]
  27. De Oliveira, É.M.; Beltrão, E.M.B.; Scavuzzi, A.M.L.; Barros, J.F.; Lopes, A.C.S. High Plasmid Variability, and the Presence of Incfib, Incq, Inca/c, Inchi1b, and Incl/m in Clinical Isolates of Klebsiella pneumoniae with Blakpc and Blandm from Patients at a Public Hospital in Brazil. Rev. Soc. Bras. Med. Trop. 2020, 53, e20200397. [Google Scholar] [CrossRef]
  28. Palmeiro, J.K.; de Souza, R.F.; Schörner, M.A.; Passarelli-Araujo, H.; Grazziotin, A.L.; Vidal, N.M.; Venancio, T.M.; Dalla-Costa, L.M. Molecular Epidemiology of Multidrug-Resistant Klebsiella pneumoniae Isolates in a Brazilian Tertiary Hospital. Front. Microbiol. 2019, 10, 1669. [Google Scholar] [CrossRef] [Green Version]
  29. Vivan, A.C.P.; Rosa, J.F.; Rizek, C.F.; Pelisson, M.; Costa, S.F.; Hungria, M.; Kobayashi, R.K.T.; Vespero, E.C. Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolates from a University Hospital in Brazil. J. Infect. Dev. Ctries. 2017, 11, 379–386. [Google Scholar] [CrossRef]
  30. Biberg, C.A.; Rodrigues, A.C.S.; do Carmo, S.F.; Chaves, C.E.V.; Gales, A.C.; Chang, M.R. KPC-2-Producing Klebsiella pneumoniae in a Hospital in the Midwest Region of Brazil. Braz. J. Microbiol. 2015, 46, 501–504. [Google Scholar] [CrossRef] [Green Version]
  31. Nava, R.G.; Oliveira-Silva, M.; Nakamura-Silva, R.; Pitondo-Silva, A.; Vespero, E.C. New Sequence Type in Multidrug-Resistant Klebsiella pneumoniae Harboring the Bla NDM-1 -Encoding Gene in Brazil. Int. J. Infect. Dis. 2019, 79, 101–103. [Google Scholar] [CrossRef] [Green Version]
  32. Brasiliense, D.; Cayô, R.; Streling, A.P.; Nodari, C.S.; Barata, R.R.; Lemos, P.S.; Massafra, J.M.; Correa, Y.; Magalhães, I.; Gales, A.C.; et al. Diversity of Metallo-β-Lactamase-Encoding Genes Found in Distinct Species of Acinetobacter Isolated from the Brazilian Amazon Region. Mem. Inst. Oswaldo Cruz 2019, 114, e190020. [Google Scholar] [CrossRef] [Green Version]
  33. Campos, J.C. Characterization of Tn3000, a Transposon Responsible for BlaNDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India. Antimicrob. Agents Chemother. 2015, 59, 7387–7395. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  34. Pillonetto, M.; Arend, L.; Vespero, E.C.; Pelisson, M.; Chagas, T.P.G.; Carvalho-Assef, A.P.D.A.; Asensi, M.D. First Report of NDM-1-Producing Acinetobacter Baumannii Sequence Type 25 in Brazil. Antimicrob. Agents Chemother. 2014, 58, 7592–7594. [Google Scholar] [CrossRef] [Green Version]
  35. Quiles, M.G.; Rocchetti, T.T.; Fehlberg, L.C.; Kusano, E.J.U.; Chebabo, A.; Pereira, R.M.G.; Gales, A.C.; Pignatari, A.C.C. Unusual Association of NDM-1 with KPC-2 and ArmA among Brazilian Enterobacteriaceae Isolates. Braz. J. Med. Biol. Res. 2015, 48, 174–177. [Google Scholar] [CrossRef] [Green Version]
  36. Góttig, S.; Hamprecht, A.G.; Christ, S.; Kempf, V.A.J.; Wichelhaus, T.A. Detection of NDM-7 in Germany, a New Variant of the New Delhi Metallo-β-Lactamase with Increased Carbapenemase Activity. J. Antimicrob. Chemother. 2013, 68, 1737–1740. [Google Scholar] [CrossRef] [Green Version]
  37. Cuzon, G.; Bonnin, R.A.; Nordmann, P. First Identification of Novel NDM Carbapenemase, NDM-7, in Escherichia Coli in France. PLoS ONE 2013, 8, e61322. [Google Scholar] [CrossRef]
  38. Shao, C.; Hao, Y.; Wang, Y.; Jiang, M.; Jin, Y. Genotypic and Phenotypic Characterization of BlaNDM–7-Harboring IncX3 Plasmid in a ST11 Klebsiella pneumoniae Isolated From a Pediatric Patient in China. Front. Microbiol. 2020, 11, 576823. [Google Scholar] [CrossRef]
  39. Mizuno, Y.; Yamaguchi, T.; Matsumoto, T. A First Case of New Delhi Metallo-β-Lactamase-7 in an Escherichia Coli ST648 Isolate in Japan. J. Infect. Chemother. 2014, 20, 814–816. [Google Scholar] [CrossRef]
  40. Shankar, C.; Kumar, S.; Venkatesan, M.; Veeraraghavan, B. Emergence of ST147 Klebsiella pneumoniae Carrying BlaNDM-7 on IncA/C2 with OmpK35 and OmpK36 Mutations in India. J. Infect. Public Health 2019, 12, 741–743. [Google Scholar] [CrossRef] [PubMed]
  41. Ahmad, N.; Ali, S.M.; Khan, A.U. Detection of New Delhi Metallo-β-Lactamase Variants NDM-4, NDM-5, and NDM-7 in Enterobacter Aerogenes Isolated from a Neonatal Intensive Care Unit of a North India Hospital: A First Report. Microb. Drug Resist. 2018, 24, 161–165. [Google Scholar] [CrossRef] [PubMed]
  42. Chen, Q.; Zhou, J.; Wu, S.; Yang, Y.; Yu, D.; Wang, X.; Wu, M. Characterization of the IncX3 Plasmid Producing BlaNDM–7 From Klebsiella pneumoniae ST34. Front. Microbiol. 2020, 11, 1885. [Google Scholar] [CrossRef]
  43. Kumarasamy, K.; Kalyanasundaram, A. Emergence of Klebsiella pneumoniae Isolate Co-Producing NDM-1 with KPC-2 from India. J. Antimicrob. Chemother. 2012, 67, 243–244. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  44. Han, R.; Shi, Q.; Wu, S.; Yin, D.; Peng, M.; Dong, D.; Zheng, Y.; Guo, Y.; Zhang, R.; Hu, F. Dissemination of Carbapenemases (KPC, NDM, OXA-48, IMP, and VIM) Among Carbapenem-Resistant Enterobacteriaceae Isolated From Adult and Children Patients in China. Front. Cell. Infect. Microbiol. 2020, 10, 314. [Google Scholar] [CrossRef] [PubMed]
  45. Sattar, H.; Toleman, M.; Nahid, F.; Zahra, R. Co-Existence of BlaNDM-1 and BlaKPC-2 in Clinical Isolates of Klebsiella pneumoniae from Pakistan. J. Chemother. 2016, 28, 346–349. [Google Scholar] [CrossRef]
  46. Tekeli, A.; Dolapci, I.; Evren, E.; Oguzman, E.; Karahan, Z.C. Characterization of Klebsiella pneumoniae Coproducing KPC and NDM-1 Carbapenemases from Turkey. Microb. Drug Resist. 2020, 26, 118–125. [Google Scholar] [CrossRef]
  47. Gao, H.; Liu, Y.; Wang, R.; Wang, Q.; Jin, L.; Wang, H. The Transferability and Evolution of NDM-1 and KPC-2 Co-Producing Klebsiella pneumoniae from Clinical Settings. EBioMedicine 2020, 51, 102599. [Google Scholar] [CrossRef] [Green Version]
  48. Nordmann, P.; Poirel, L.; Carrër, A.; Toleman, M.A.; Walsh, T.R. How to Detect NDM-1 Producers. J. Clin. Microbiol. 2011, 49, 718–721. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  49. Programa Nacional de Control de Calidad en Bacteriologia INEI_ANLIS “Dr. Carlos G. Malbran”. Available online: http://antimicrobianos.com.ar/programa-latinoamericano-de-control-de-calidad-en-bacteriologia-y-resistencia-a-los-antimicrobianos/ (accessed on 21 October 2021).
  50. Flores, C.; Bianco, K.; De Filippis, I.; Clementino, M.M.; Romão, C.M.C.P.A. Genetic Relatedness of NDM-Producing Klebsiella pneumoniae Co-Occurring VIM, KPC, and OXA-48 Enzymes from Surveillance Cultures from an Intensive Care Unit. Microb. Drug Resist. 2020, 26, 1219–1226. [Google Scholar] [CrossRef] [PubMed]
  51. Woodford, N.; Turton, J.F.; Livermore, D.M. Multiresistant Gram-Negative Bacteria: The Role of High-Risk Clones in the Dissemination of Antibiotic Resistance. FEMS Microbiol. Rev. 2011, 35, 736–755. [Google Scholar] [CrossRef] [Green Version]
  52. Andrade, L.N.; Novais, Â.; Stegani, L.M.M.; Ferreira, J.C.; Rodrigues, C.; Darini, A.L.C.; Peixe, L. Virulence Genes, Capsular and Plasmid Types of Multidrug-Resistant CTX-M(-2, -8, -15) and KPC-2-Producing Klebsiella pneumoniae Isolates from Four Major Hospitals in Brazil. Diagn. Microbiol. Infect. Dis. 2018, 91, 164–168. [Google Scholar] [CrossRef]
  53. Fuga, B.; Ferreira, M.L.; Cerdeira, L.T.; de Campos, P.A.; Dias, V.L.; Rossi, I.; Machado, L.G.; Lincopan, N.; Gontijo-Filho, P.P.; Ribas, R.M. Novel Small IncX3 Plasmid Carrying the BlaKPC-2 Gene in High-Risk Klebsiella pneumoniae ST11/CG258. Diagn. Microbiol. Infect. Dis. 2020, 96, 114900. [Google Scholar] [CrossRef]
  54. Raro, O.H.F.; da Silva, R.M.C.; Filho, E.M.R.; Sukiennik, T.C.T.; Stadnik, C.; Dias, C.A.G.; Oteo Iglesias, J.; Pérez-Vázquez, M. Carbapenemase-Producing Klebsiella pneumoniae From Transplanted Patients in Brazil: Phylogeny, Resistome, Virulome and Mobile Genetic Elements Harboring BlaKPC–2 or BlaNDM–1. Front. Microbiol. 2020, 11, 1563. [Google Scholar] [CrossRef]
  55. Chen, L.; Mathema, B.; Chavda, K.D.; DeLeo, F.R.; Bonomo, R.A.; Kreiswirth, B.N. Carbapenemase-Producing Klebsiella Pneumoniae: Molecular and Genetic Decoding. Trends Microbiol. 2014, 22, 686–696. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  56. Liu, Y.M.; Li, B.B.; Zhang, Y.Y.; Zhang, W.; Shen, H.; Li, H.; Cao, B. Clinical and Molecular Characteristics of Emerging Hypervirulent Klebsiella pneumoniae Bloodstream Infections in Mainland China. Antimicrob. Agents Chemother. 2014, 58, 5379–5385. [Google Scholar] [CrossRef] [Green Version]
  57. Palmieri, M.; Wyres, K.L.; Mirande, C.; Qiang, Z.; Liyan, Y.; Gang, C.; Goossens, H.; van Belkum, A.; Ping, L.Y. Genomic Evolution and Local Epidemiology of Klebsiella pneumoniae from a Major Hospital in Beijing, China, over a 15 Year Period: Dissemination of Known and Novel High-Risk Clones. Microb. Genomics 2021, 7, 520. [Google Scholar] [CrossRef]
  58. Monteiro, J.; Inoue, F.M.; Lobo, A.P.T.; Ibanes, A.S.; Tufik, S.; Kiffer, C.R.V. A Major Monoclonal Hospital Outbreak of NDM-1-Producing Klebsiella pneumoniae ST340 and the First Report of ST2570 in Brazil. Infect. Control Hosp. Epidemiol. 2019, 40, 492–494. [Google Scholar] [CrossRef] [Green Version]
  59. Heinz, E.; Ejaz, H.; Bartholdson Scott, J.; Wang, N.; Gujaran, S.; Pickard, D.; Wilksch, J.; Cao, H.; Haq, I.u.; Dougan, G.; et al. Resistance Mechanisms and Population Structure of Highly Drug Resistant Klebsiella in Pakistan during the Introduction of the Carbapenemase NDM-1. Sci. Rep. 2019, 9, 2392. [Google Scholar] [CrossRef] [Green Version]
  60. Chung The, H.; Karkey, A.; Pham Thanh, D.; Boinett, C.J.; Cain, A.K.; Ellington, M.; Baker, K.S.; Dongol, S.; Thompson, C.; Harris, S.R.; et al. A High-resolution Genomic Analysis of Multidrug-resistant Hospital Outbreaks of Klebsiella pneumoniae. EMBO Mol. Med. 2015, 7, 227–239. [Google Scholar] [CrossRef] [PubMed]
  61. Martins, W.; Nicolas, M.F.; Yu, Y.; Li, M.; Dantas, P.; Sands, K.; Portal, E.; Almeida, L.; Vasconcelos, A.; Medeiros, E.A.; et al. Clinical and Molecular Description of a High-Copy IncQ1 KPC-2 Plasmid Harbored by the International ST15 Klebsiella pneumoniae Clone. mSphere 2020, 5, e00756-20. [Google Scholar] [CrossRef]
  62. Campana, E.H.; Montezzi, L.F.; Paschoal, R.P.; Picão, R.C. NDM-Producing Klebsiella pneumoniae ST11 Goes to the Beach. Int. J. Antimicrob. Agents 2017, 49, 119–121. [Google Scholar] [CrossRef] [PubMed]
  63. D’Apolito, D.; Arena, F.; Conte, V.; De Angelis, L.H.; Di Mento, G.; Carreca, A.P.; Cuscino, N.; Russelli, G.; Iannolo, G.; Barbera, F.; et al. Phenotypical and Molecular Assessment of the Virulence Potential of KPC-3-Producing Klebsiella pneumoniae ST392 Clinical Isolates. Microbiol. Res. 2020, 240, 126551. [Google Scholar] [CrossRef] [PubMed]
  64. Di Mento, G.; Cuscino, N.; Carcione, C.; Cardinale, F.; Conaldi, P.G.; Douradinha, B. Emergence of a Klebsiella pneumoniae ST392 Clone Harbouring KPC-3 in an Italian Transplantation Hospital. J. Hosp. Infect. 2018, 98, 313–314. [Google Scholar] [CrossRef]
  65. Bocanegra-Ibarias, P.; Garza-González, E.; Morfín-Otero, R.; Barrios, H.; Villarreal-Treviño, L.; Rodríguez-Noriega, E.; Garza-Ramos, U.; Petersen-Morfin, S.; Silva-Sanchez, J. Molecular and Microbiological Report of a Hospital Outbreak of NDM-1-Carrying Enterobacteriaceae in Mexico. PLoS ONE 2017, 12, e179651. [Google Scholar] [CrossRef]
  66. Rojas, L.J.; Wright, M.S.; De La Cadena, E.; Motoa, G.; Hujer, K.M.; Villegas, M.V.; Adams, M.D.; Bonomo, R.A. Initial Assessment of the Molecular Epidemiology of BlaNDM-1 in Colombia. Antimicrob. Agents Chemother. 2016, 60, 4346–4350. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  67. Pajand, O.; Darabi, N.; Arab, M.; Ghorbani, R.; Bameri, Z.; Ebrahimi, A.; Hojabri, Z. The Emergence of the Hypervirulent Klebsiella pneumoniae (HvKp) Strains among Circulating Clonal Complex 147 (CC147) Harbouring Bla NDM/OXA-48 Carbapenemases in a Tertiary Care Center of Iran. Ann. Clin. Microbiol. Antimicrob. 2020, 19, 12. [Google Scholar] [CrossRef]
  68. Mills, J.P.; Rojas, L.J.; Marshall, S.H.; Rudin, S.D.; Hujer, A.M.; Nayak, L.; Bachman, M.A.; Bonomo, R.A.; Kaye, K.S. Risk Factors for and Mechanisms of COlistin Resistance among Enterobacterales: Getting at the CORE of the Issue. Open Forum Infect. Dis. 2021, 8, ofab145. [Google Scholar] [CrossRef] [PubMed]
  69. Flores-Valdez, M.; Ares, M.A.; Rosales-Reyes, R.; Torres, J.; Girón, J.A.; Weimer, B.C.; Mendez-Tenorio, A.; De la Cruz, M.A. Whole Genome Sequencing of Pediatric Klebsiella pneumoniae Strains Reveals Important Insights Into Their Virulence-Associated Traits. Front. Microbiol. 2021, 12, 711577. [Google Scholar] [CrossRef] [PubMed]
  70. Bowers, J.R.; Lemmer, D.; Sahl, J.W.; Pearson, T.; Driebe, E.M.; Wojack, B.; Saubolle, M.A.; Engelthaler, D.M.; Keim, P. KlebSeq, a Diagnostic Tool for Surveillance, Detection, and Monitoring of Klebsiella Pneumoniae. J. Clin. Microbiol. 2016, 54, 2582–2596. [Google Scholar] [CrossRef] [Green Version]
  71. Chen, L.; Peirano, G.; Lynch, T.; Chavda, K.D.; Gregson, D.B.; Church, D.L.; Conly, J.; Kreiswirth, B.N.; Pitout, J.D. Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing BlaNDM-7in Enterobacteriaceae from Calgary, Canada. Antimicrob. Agents Chemother. 2016, 60, 1258–1263. [Google Scholar] [CrossRef] [Green Version]
  72. Chen, D.; Xiao, L.; Hong, D.; Zhao, Y.; Hu, X.; Shi, S.; Chen, F. Epidemiology of Resistance of Carbapenemase-Producing Klebsiella pneumoniae to Ceftazidime-Avibactam in a Chinese Hospital. J. Appl. Microbiol. 2021, 1–7. [Google Scholar] [CrossRef] [PubMed]
  73. CLSI. Clinical and Laboratory Standards Institute. In Performance Standards for Antimicrobial Susceptibility Testing, 30th ed.; Document M100; CLSI: Wayne, PA, USA, 2020. [Google Scholar]
  74. Food and Drug Administration (FDA). Tigecycline–Injection Products. 2019; p. 15. Available online: https://www.fda.gov/drugs/development-resources/tigecycline-injection-products (accessed on 21 October 2021).
  75. Magiorakos, A.P.; Srinivasan, A.; Carey, R.B.; Carmeli, Y.; Falagas, M.E.; Giske, C.G.; Harbarth, S.; Hindler, J.F.; Kahlmeter, G.; Olsson-Liljequist, B.; et al. Multidrug-Resistant, Extensively Drug-Resistant and Pandrug-Resistant Bacteria: An International Expert Proposal for Interim Standard Definitions for Acquired Resistance. Clin. Microbiol. Infect. 2012, 18, 268–281. [Google Scholar] [CrossRef] [Green Version]
  76. Mendes, R.E.; Castanheira, M.; Toleman, M.A.; Sader, H.S.; Jones, R.N.; Walsh, T.R. Characterization of an Integron Carrying BlaIMF-1 and a New Aminoglycoside Resistance Gene, Aac(6′)-31, and Its Dissemination among Genetically Unrelated Clinical Isolates in a Brazilian Hospital. Antimicrob. Agents Chemother. 2007, 51, 2611–2614. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  77. Zhang, R.; Hu, Y.Y.; Yang, X.F.; Gu, D.X.; Zhou, H.W.; Hu, Q.F.; Zhao, K.; Yu, S.F.; Chen, G.X. Emergence of NDM-Producing Non-Baumannii Acinetobacter Spp. Isolated from China. Eur. J. Clin. Microbiol. Infect. Dis. 2014, 33, 853–860. [Google Scholar] [CrossRef] [PubMed]
  78. Diancourt, L.; Passet, V.; Verhoef, J.; Grimont, P.A.D.; Brisse, S. Multilocus Sequence Typing of Klebsiella pneumoniae Nosocomial Isolates. J. Clin. Microbiol. 2005, 43, 4178–4182. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  79. Francisco, A.P.; Vaz, C.; Monteiro, P.T.; Melo-Cristino, J.; Ramirez, M.; Carriço, J.A. PHYLOViZ: Phylogenetic Inference and Data Visualization for Sequence Based Typing Methods. BMC Bioinform. 2012, 13, 87. [Google Scholar] [CrossRef] [PubMed] [Green Version]
Antibiotics 10 01527 g001 550
Figure 1. Minimum spanning tree (MST) of the 23 NDM-producing K. pneumoniae isolates genotyped by MLST. Each circle represents a different ST, and the size of the circle is proportional to the number of isolates related to the respective ST. The number next to the lines represents the number of loci variations between STs.
Figure 1. Minimum spanning tree (MST) of the 23 NDM-producing K. pneumoniae isolates genotyped by MLST. Each circle represents a different ST, and the size of the circle is proportional to the number of isolates related to the respective ST. The number next to the lines represents the number of loci variations between STs.
Antibiotics 10 01527 g001
Table
Table 1. Epidemiological and genotyping characteristics of the 23 NDM-producing K. pneumoniae isolates from different healthcare institutions, state of Pará, Brazilian Amazon region.
Table 1. Epidemiological and genotyping characteristics of the 23 NDM-producing K. pneumoniae isolates from different healthcare institutions, state of Pará, Brazilian Amazon region.
Isolate IDHospitalWardClinical SpecimenDateSTCCHRCNDM Subtype
46956(H1)Pediatric clinicBlood20 August 20184398Singleton−NDM-7
47098(H2)Adult ICUBronchoalveolar lavage24 August 20181515+NDM-1
47398(H3)Adult ICUUrine11 September 2018340258+NDM-1
49684(H4)Adult clinicUrine3 January 20191515+NDM-1
50177(H1)Pediatric clinicBlood19 February 20191264258+NDM-7
50467(H5)Adult ICURectal swab18 March 20191515+NDM-7
50933(H3)Pediatric clinicUrine27 March 2019392147+NT
50937(H3)Pediatric clinicUrine15 April 201911258+NDM-1
50938(H6)Adult clinicAbdominal abscess secretion10 April 201911258+NDM-1
50942(H2)Adult clinicWound secretion22 April 20191515+NDM-1
51999(H2)Adult clinicUrine10 June 201911258+NDM-7
51887(H3)Pediatric ICUTracheal secretion14 May 201911258+NDM-1
52012(H2)Adult ICUTracheal secretion21 June 20193512Singleton−NDM-7
54200(H7)Adult clinicUrine29 October 20193449Singleton−NDM-7
56585(H8)Adult ICURectal swab29 May 202014011401−NDM-7
57319(H3)Pediatric ICUSoft tissue secretion20 November 202011258+NDM-7
57351(H1)Pediatric clinicBlood1 February 202111258+NDM-7
57352(H9)Adult clinicUrine24 January 202111258+NDM-7
57387(H3)Pediatric ICUNasopharyngeal secretion19 January 202111258+NDM-7
57413(H3)Pediatric ICURectal swab28 January 202111258+NDM-7
57414(H3)Adult clinicUrine26 January 20211515+NDM-1
57420(H9)Adult ICUTracheal secretion28 January 202111258+NDM-7
57090(H2)Adult clinicPeritoneal fluid9 November 2020138138−NDM-7
ICU: Intensive care unit; ST: Sequence type; CC: Clonal complex; HRC: high risk clone; NT: not subtyped.
Table
Table 2. Phenotypical and molecular susceptibility features of MDR K. pneumoniae isolates from different healthcare institutions, state of Pará, Brazilian Amazon region.
Table 2. Phenotypical and molecular susceptibility features of MDR K. pneumoniae isolates from different healthcare institutions, state of Pará, Brazilian Amazon region.
Isolate IDMIC (µg/mL)Carbapenemase
Gene		mCIMeCIM
AMPSAMTZPCXMFOXCAZCROFEPETPIMPMEMAMKGENCIPTGC
46956>16>16>64>32>32>32>32>32>4>8>8≤2>8≤0.25≤0.5blaNDM-7++
47098>16>16>64>32>32>32>32>32>4≤0.2548>8>2≤0.5blaNDM-1++
47398>16>16>64>32>32>32>32>32>4>8>816>8>22blaNDM-1++
49684>16>16>64>32>32>32>32>32>4>8>816≤1>21blaNDM-1++
50177>16>16>64>32>32>32>32>32>4>8>8≤2>8>22blaNDM-7++
504673232128646464643241616≤216≥41blaNDM-7++
50933>16>16>64>32>32>32>32>32>4>8>8≤2>8>2>4blaNDM *++
50937>16>16>64>32>32>32>32>32>4>8>8162>22blaNDM-1++
50938>16>16>64>32>32>32>32>32>4>8>816≤1>24blaNDM-1/blaKPC-2+−
50942>16>16>64>32>32>32>32>32>4>8>816≤1>22blaNDM-1++
51999>16>16>64>32>32>32>32>32>4>8>8>32>8>2>4blaNDM-7++
51887>16>16>64>32>32>32>32>32>4>8>816≤1>22blaNDM-1/blaKPC-2+−
52012>16>16>64>32>32>32>32>32>4>8>816≤1>2>4blaNDM-7++
54200>16>16>64>32>32>32>32>32>4>8>8≤2≤121blaNDM-7++
56585>16>16>64>32>32>32>32>32>4>8>8≤2≤11<0.5blaNDM-7++
57319323212864646464648≥16≥1616≥1642blaNDM-7++
57351>16>16>64>32>32>32>32>32>4>8>84>8>22blaNDM-7++
57352>16>16>64>32>32>32>32>32>48>8≤2>8>22blaNDM-7++
57387>16>16>64>32>32>32>32>32>4>8>8≤2≤1>22blaNDM-7/blaKPC-2+−
57413>16>16>64>32>32>32>32>32>4>8>8≤2≤1>22blaNDM-7/blaKPC-2++
57414>16>16>64>32>32>32>32>32>4>8>816≤1>22blaNDM-1++
57420>16>16>64>32>32>32>32>32>48>8≤28>24blaNDM-7++
57090>16>16>64>32>32>32>32>32>4>8>8≤2≤1≤0.251blaNDM-7++
AMK: amikacin; AMP: ampicillin; CAZ: ceftazidime; CIP: ciprofloxacin; CRO: ceftriaxone; CXM: cefuroxime; ERT: ertapenem; FEP: cefepime; FOX: cefoxitin; GEN: gentamicin; IMP: imipenem; MEM: meropenem; MIC: minimal inhibitory concentration; SAM: ampicillin/sulbactam; TGC: tigecycline; TZP: piperacillin/tazobactam. * NDM subtyping could not be performed.
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Rodrigues, Y.C.; Lobato, A.R.F.; Quaresma, A.J.P.G.; Guerra, L.M.G.D.; Brasiliense, D.M. The Spread of NDM-1 and NDM-7-Producing Klebsiella pneumoniae Is Driven by Multiclonal Expansion of High-Risk Clones in Healthcare Institutions in the State of Pará, Brazilian Amazon Region. Antibiotics 2021, 10, 1527. https://doi.org/10.3390/antibiotics10121527

AMA Style

Rodrigues YC, Lobato ARF, Quaresma AJPG, Guerra LMGD, Brasiliense DM. The Spread of NDM-1 and NDM-7-Producing Klebsiella pneumoniae Is Driven by Multiclonal Expansion of High-Risk Clones in Healthcare Institutions in the State of Pará, Brazilian Amazon Region. Antibiotics. 2021; 10(12):1527. https://doi.org/10.3390/antibiotics10121527

Chicago/Turabian Style

Rodrigues, Yan Corrêa, Amália Raiana Fonseca Lobato, Ana Judith Pires Garcia Quaresma, Lívia Maria Guimarães Dutra Guerra, and Danielle Murici Brasiliense. 2021. "The Spread of NDM-1 and NDM-7-Producing Klebsiella pneumoniae Is Driven by Multiclonal Expansion of High-Risk Clones in Healthcare Institutions in the State of Pará, Brazilian Amazon Region" Antibiotics 10, no. 12: 1527. https://doi.org/10.3390/antibiotics10121527

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop