Dynamic Changes in Proteome during Yak Oocyte Maturation Analyzed Using iTRAQ Technology
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Collection and In Vitro Culture of Oocytes
2.2. Experimental Method
2.2.1. Protein Extraction
2.2.2. Quality Control of Protein Extraction
2.2.3. Proteolytic Digestion
2.2.4. Peptide Labeling
2.2.5. Peptide Separation
2.2.6. High-Performance Liquid Phase
2.2.7. Mass Spectrometry Detection
2.3. Bioinformatics Analysis
2.4. Immunofluorescence Verification
3. Results
3.1. Mass Spectrometry Identification Results
3.1.1. Basic Identification Information
3.1.2. Protein Relative Molecular Mass Distribution
3.1.3. Peptide Sequence Length Distribution and Protein Abundance Ratio
3.2. Bioinformatics Analysis of Differential Proteins
3.2.1. GO Enrichment Analysis of Differential Proteins
3.2.2. KOG Analysis of Differential Proteins
3.2.3. KEGG Analysis of Differential Proteins
3.3. Immunofluorescence Verification
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample Name | Total Spectra | Spectra | Unique Spetra | Peptide | Unique Peptide | Protein |
---|---|---|---|---|---|---|
Bos_grunniens | 834,458 | 56,447 | 50,116 | 28,204 | 26,745 | 6075 |
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Ma, X.; Wang, M.; Wang, J.; Zhang, Q.; Pu, S.; Wang, R.; Yu, S.; Wang, L.; Pan, Y. Dynamic Changes in Proteome during Yak Oocyte Maturation Analyzed Using iTRAQ Technology. Animals 2023, 13, 2085. https://doi.org/10.3390/ani13132085
Ma X, Wang M, Wang J, Zhang Q, Pu S, Wang R, Yu S, Wang L, Pan Y. Dynamic Changes in Proteome during Yak Oocyte Maturation Analyzed Using iTRAQ Technology. Animals. 2023; 13(13):2085. https://doi.org/10.3390/ani13132085
Chicago/Turabian StyleMa, Xin, Meng Wang, Jinglei Wang, Qian Zhang, Sisi Pu, Rui Wang, Sijiu Yu, Libin Wang, and Yangyang Pan. 2023. "Dynamic Changes in Proteome during Yak Oocyte Maturation Analyzed Using iTRAQ Technology" Animals 13, no. 13: 2085. https://doi.org/10.3390/ani13132085