Application of Selenium Can Alleviate the Stress of Cadmium on Rapeseed at Different Growth Stages in Soil
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Experimentation
2.3. Plant Sampling
2.4. Soil Sampling
2.5. DNA Extract and PCR Products Mixing and Purification
2.6. High-Throughput Sequencing
2.7. Statistical Analysis
3. Results
3.1. Effects of Different Amounts of Selenium Application on Agronomic Characteristics and Yield of Rapeseed during Maturity
3.2. Effects of Different Amounts of Selenium on Cadmium Contents in Different Parts of Rapeseed
3.3. Diversity and Community Differences in Rhizosphere Microbial Communities
3.4. Effect of Root Selenium Treatment on Soil Bacterial Community Structure
3.5. Effects of Non-Selenium Treatment on Different Soil Microbial Communities
3.6. Effects of Selenium Treatment on Different Soil Microbial Communities
3.7. Effects of Selenium Treatment on Soil Metabolic Pathways
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Treatment | Silicle Number per Plant | Seeds per Silicle | Seed Weight (mg) | Yield (kg/hm2) |
---|---|---|---|---|
CK | 610.4 ± 29.5 b | 23.26 ± 0.81 a | 2.92 ± 0.18 a | 2280.18 ± 82.51 b |
L | 676.4 ± 22.1 a | 24.38 ± 0.59 a | 3.04 ± 0.23 a | 2757.23 ± 148.21 a |
H | 611.8 ± 4.9 b | 23.53 ± 0.12 a | 2.94 ± 0.25 a | 2327.78 ± 40.43 ab |
Sample | Raw_Tags | Clean_Tags | Coverage |
---|---|---|---|
B_CK | 105,498 | 101,630 | 96.33 |
B_H | 361,809 | 343,433 | 94.92 |
B_L | 91,700 | 89,008 | 97.06 |
F_CK | 124,037 | 120,711 | 97.32 |
F_H | 185,158 | 181,078 | 97.80 |
F_L | 61,673 | 60,132 | 97.50 |
M_CK | 512,856 | 496,359 | 96.78 |
M_H | 216,585 | 210,290 | 97.09 |
M_L | 350,507 | 336,036 | 95.87 |
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Nie, L.; Zhou, B.; Hong, B.; Wang, X.; Chang, T.; Guan, C.; Guan, M. Application of Selenium Can Alleviate the Stress of Cadmium on Rapeseed at Different Growth Stages in Soil. Agronomy 2023, 13, 2228. https://doi.org/10.3390/agronomy13092228
Nie L, Zhou B, Hong B, Wang X, Chang T, Guan C, Guan M. Application of Selenium Can Alleviate the Stress of Cadmium on Rapeseed at Different Growth Stages in Soil. Agronomy. 2023; 13(9):2228. https://doi.org/10.3390/agronomy13092228
Chicago/Turabian StyleNie, Lingli, Bingqian Zhou, Bo Hong, Xiaodan Wang, Tao Chang, Chunyun Guan, and Mei Guan. 2023. "Application of Selenium Can Alleviate the Stress of Cadmium on Rapeseed at Different Growth Stages in Soil" Agronomy 13, no. 9: 2228. https://doi.org/10.3390/agronomy13092228