This article is a correction to:
First Report of Coexistence of blaSFO–1 and blaNDM–1 β-Lactamase Genes as Well as Colistin Resistance Gene mcr-9 in a Transferrable Plasmid of a Clinical Isolate of Enterobacter hormaechei
Wenxiu Ai1†
Ying Zhou2,3†
Bingjie Wang2,3
Qing Zhan2,3Longhua Hu4Yanlei Xu2,3Yinjuan Guo2,3Liangxing Wang1*
Fangyou Yu2,3*Xiaolong Li5*- 1Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
- 2Department of Clinical Laboratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China
- 3Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China
- 4Jiangxi Provincial Key Laboratory of Medicine, Clinical Laboratory of the Second Affiliated Hospital of Nanchang University, Nanchang, China
- 5Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
by Ai, W., Zhou, Y., Wang, B., Zhan, Q., Hu, L., Xu, Y., Guo, Y., Wang, L., Yu, F., and Li, X. (2021). Front. Microbiol. 12:676113. doi: 10.3389/fmicb.2021.676113
In the original article, there were two errors in the main text. The sentence “The conjugation frequency was calculated as the number of transconjugants per recipient” should have read “The conjugation frequency was calculated as the number of transconjugants per donor cell.”
A correction has been made to Materials and Methods, Conjugation Experiment:
“The horizontal transferability of blaSFO−1, blaNDM−1, and mcr-9 was examined using conjugation assay. The E. hormaechei 1575 was used as donor strain, and the E. coli EC600 (rifampicin-resistant) was used as the recipient strain. The donors and recipients were cultured to the logarithmic phase (OD600 = 0.4–0.6), mixed in a 1:1 ratio, centrifuged at 8,000 g for 1 min, and resuspended them in 20 μl of Luria Bertani (LB) broth. The resuspension was spotted on the LB plates and incubated overnight at 37°C. The spots were then transferred to 15-ml centrifuge tubes and washed with 3 ml of LB broth. Subsequently, the serial dilutions were plated onto MH agar plates containing cefotaxime (8 μg/ml) and rifampicin (200 μg/ml). The donor cells and recipient cells were used separately as controls to ensure the effectiveness of the screening plate antibiotics. All transconjugants were confirmed by PCR for the presence of blaSFO−1, blaNDM−1, and mcr-9 genes. Transconjugants were subjected to susceptibility assays. The conjugation frequency was calculated as the number of transconjugants per donor cell.”
In addition, the sentence “The blaSFO−1 was located in an IS26 integron” should have read “The blaSFO−1 was located in an IS26 composite transposon.”
A correction has been made to Discussion, second paragraph:
“Previous studies showed that multiple resistance transfer of plasmids could result from rare gene capture events mediated by different mobile genetic elements, clustering, and combinatorial evolution of resistance genes and related mobile elements (Partridge and Tsafnat, 2018). Through the WGS and comparative genomics, we clarified that the key to mediating the antibiotic resistance of this strain was the p1575-1 resistant plasmid. The p1575-1 identified in this study was an IncHI2 conjugative plasmid, representing one of the most frequently encountered plasmid types in Enterobacteriaceae (Carattoli, 2009). Notably, IncHI2 plasmids are also broad-host-range, large (>250 kb) conjugative plasmids that mobilize metal and drug resistance genes within gram-negative pathogens (Bertrand et al., 2006; Novais et al., 2006; Roy Chowdhury et al., 2019). Meanwhile, IncHI2-ST1 plasmids always contributed to the dissemination of carbapenemase-encoding genes and are also reported frequently to play a critical role in the evolution of complex resistance phenotypes within disease-causing strains of Enterobacteriaceae (Roy Chowdhury et al., 2019). Moreover, IncHI2 plasmids contain the conjugal transfer gene regions tra1 and tra2, likely contributing to the spread of resistance in the environment (Sherburne et al., 2000). In this study, we analyzed the conjugative modules of the p1575-1 plasmid and evaluated its mobility with conjugation assay. Like the classical IncHI2 plasmids, the p1575-1 plasmid held a complete conjugative system, and the conjugation frequencies ranged from 0.5 × 10−6 to 2 × 10−6 per donor cell. The IncHI2-type conjugative plasmids harboring mcr-9 were also discovered previously, and the conjugation frequencies of those plasmid were 10−4 (Lin et al., 2020) or 2.03 × 10−7 (−5.42 × 10−8) (Cha et al., 2020), which were similar to our findings. Through the analysis, we identified the complete conjugative modules on the plasmid p1575-1, strongly suggesting that p1575-1 could be transferred autonomously. In addition to the conjugative plasmids, the capture, accumulation, and dissemination of resistance genes are largely due to the actions of mobile genetic elements, including insertion sequences, transposons, gene cassettes, and integrons. In this study, we found that all these three resistance genes were flanked by several mobile elements. The blaSFO−1 was located in an IS26 composite transposon. IS6 family elements IS26 have played a pivotal role in the dissemination of resistance determinants in Gram-negative bacteria; thus, blaSFO−1 held the potential to transfer to other strains. AmpR, a class of DNA-binding regulatory protein, belongs to the LysR family of transcriptional regulators (Henikoff et al., 1988; Bartowsky and Normark, 1993). AmpR is confirmed to be a transcriptional activator in the presence of certain β-lactam antibiotics in the culture medium and a repressor in their absence (Lindberg et al., 1988). The presence of ampR seems to be a disadvantage for the host strain because E. cloacae become highly resistant to β-lactams (Matsumoto and Inoue, 1999). The movement of IS26 is originally demonstrated to occur by replicative transposition. Moreover, the blaSFO-1 genes in previous identifications were located on non-conjugative plasmids (Guo et al., 2012). In our study, the conjugative blaSFO−1-blaNDM−1-mcr-9-bearing plasmid belonged to IncHI2, which is a kind of broad-host-range mobile plasmid and might greatly accelerate the dissemination of the blaSFO−1 genes. Previous reports showed that the blaNDM−1 genes in Enterobacteriaceae were usually on 50- to 200-kb plasmids belonging to IncL/M, IncHI1, IncFIIs, IncF, or untypable (Ahmad et al., 2018). ISAba125 and Tn125 are always associated with the blaNDM−1 gene. Upstream of the blaNDM−1 gene, a truncated insertion sequence, ISAba125, was identified, which provides a promoter for the expression of blaNDM−1 (Carattoli et al., 2012), and the presence of ble andΔtnpA genes suggests a possible hypothesis that blaNDM−1 originates from Acinetobacter baumannii (Poirel et al., 2012; Toleman et al., 2012). Besides, phosphoribosylanthranilate isomerase gene trpF was identified in the downstream sequences of the bleMBL gene (Liu et al., 2013). In addition, qnrS1 in IS26–blaLAP−2-qnrS1–IS26 unit (3D) was also found, consistent with our AST results. In the IncHI2 plasmid, the mcr-9 allele always inserted an IS903B element and an ISEsp1, encoding a cupin fold metalloprotein, wbuC family (Yuan et al., 2019; Börjesson et al., 2020), which was consistent with our results. Because mcr-9.1 was located between IS903B and IS26, these flanking sequences can also be potentially transferred to other bacteria along with mcr-9.1. All results indicated that the resistant plasmid carried by E. hormaechei 1575 can be spontaneously transmitted to other strains through conjugation, which had great potential to cause clinical epidemics. qseB and qseC regulatory genes were found in association with the mcr-9 gene and played an important role in mediating polymyxin resistance (Chavda et al., 2019; Kieffer et al., 2019). The lack of two key regulators (qseB and qseC) may explain why E. hormaechei 1575 carrying mcr-9 did not exhibit a high resistance level to colistin (MIC, 2 μg/ml). Serious importance needs to be taken on this phenomenon.”
The authors apologize for these errors and state that they do not change the scientific conclusions of the article in any way. The original article has been updated.
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Keywords: Enterobacter hormaechei, plasmid, blaSFO−1, blaNDM−1, mcr-9, IncHI2, WGS, mobile elements
Citation: Ai W, Zhou Y, Wang B, Zhan Q, Hu L, Xu Y, Guo Y, Wang L, Yu F and Li X (2021) Corrigendum: First Report of Coexistence of blaSFO-1 and blaNDM-1 β-Lactamase Genes as Well as Colistin Resistance Gene mcr-9 in a Transferrable Plasmid of a Clinical Isolate of Enterobacter hormaechei. Front. Microbiol. 12:741628. doi: 10.3389/fmicb.2021.741628
Received: 15 July 2021; Accepted: 03 September 2021;
Published: 28 September 2021.
Edited and reviewed by: Che-Hsin Lee, National Sun Yat-sen University, Taiwan
Copyright © 2021 Ai, Zhou, Wang, Zhan, Hu, Xu, Guo, Wang, Yu and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Liangxing Wang, 38805@163.com; Fangyou Yu, wzjxyfy@163.com; Xiaolong Li, lixiaolongcq@163.com
†These authors have contributed equally to this work