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    J Korean Soc Radiol. 2011 May;64(5):481-489. Korean.
    Published online May 31, 2011.
    https://doi.org/10.3348/jksr.2011.64.5.481
    Copyright © 2011 The Korean Society of Radiology
    Original Article

    Measurement of Trabecular Bone Parameters in Porcine Vertebral Bodies Using Multidetector CT: Evaluation of Reproducibility of 3-Dimensional CT Histomorphometry

    Sang Bu An, M.D.,2 Sung Hwan Hong, M.D.,1 Jin Mo Goo, M.D.,1 Kyung Chul Moon, M.D.,3 and Kwang Gi Kim, M.D.4
      • 1Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine, Korea.
      • 2Department of Radiology, National Cancer Center, Korea.
      • 3Department of Pathology, Seoul National University Hospital, Seoul National University College of Medicine, Korea.
      • 4Department of Biomedical Engineering, Division of Basic and Applied Sciences, National Cancer Center, Korea.
    • Address reprint requests to: Sung Hwan Hong, M.D., Department of Radiology, Seoul National University Hospital, 28 Yongon-dong, Chongno-gu, Seoul 110-744, Korea. Tel. 82-2-2072-3217, Fax. 82-2-743-6385, Email: drhong@snu.ac.kr
    Received January 14, 2011; Accepted March 06, 2011.

    Abstract

    Purpose

    To evaluate the reproducibility of 3-dimensional histomorphometry for the microarchitecture analysis of trabecular bone parameters using multidetector computed tomography (MDCT).

    Materials and Methods

    Thirty-six specimens from porcine vertebral bodies were imaged five times with a 64-detector row MDCT system using the same scan protocols. Locations of the specimens were nearly identical through the scans. Three-dimensional structural parameters of trabecular bone were derived from the five data sets using image analyzing software. The features measured by the analysis programs were trabecular bone volume, trabecular bone volume/tissue volume, trabecular thickness, trabecular separation, trabecular number, trabecular bone pattern factor, structural model index.

    Results

    The structural trabecular parameters showed excellent reproducibility through repeated scanning. Intraclass correlation coefficients of all seven structural parameters were in the range of 0.998 to 1.000. Coefficients of variation of the six structural parameters, excluding structural model index, were not over 1.6%.

    Conclusion

    The measurement of the trabecular structural parameters using multidetector CT and three-dimensional histomophometry analysis program was validated and showed excellent reproducibility. This method could be used as a noninvasive and easily available test in a clinical setting.

    Keywords
    Computed tomography (CT); Bone microarchitecture; Reproducibility

    Figures

    Fig. 1
    Bland-Altman plots of paired results of the three-dimensional structural parameters between the maximum value and minimum value.
    Note.─ BV= trabecular bone volume, BVTV= trabecular bone volume/tissue volume, Tb_Th= trabecular thickness, Tb_Sp= trabecular separation, Tb_N= trabecular number, Tb_Pf= trabecular bone pattern factor, SMI= structural model index

    Fig. 2
    Dispersion of standard deviation versus mean and coefficient of variation versus mean.
    Note.─ BV= trabecular bone volume, BVTV= trabecular bone volume/tissue volume, Tb_Th= trabecular thickness, Tb_Sp= trabecular separation, Tb_N= trabecular number, Tb_Pf= trabecular bone pattern factor, SMI= structural model index, SD= standard deviation, CV= coefficient of variation

    Tables

    Table 1
    Intraclass Correlation Coefficients (ICC) of Structural Trabecular Parameters

    Table 2
    Distribution of Coefficients of Variation for the Structural Parameters Repeatedly Scanned Five Times

    Notes

    This study was supported by grant no 04-2007-046-0 from the SNUH Research Fund

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    MeSH Terms
    Models, Structural
    Multidetector Computed Tomography
    Metrics
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