J Korean Med Sci. 1993 Aug;8(4):257-261. English.
Published online May 27, 2009.
https://doi.org/10.3346/jkms.1993.8.4.257
Published online May 27, 2009.
https://doi.org/10.3346/jkms.1993.8.4.257
Copyright © 1993 The Korean Academy of Medical Sciences
Original Article
Detection of chromosome aberrations in interphase nuclei using fluorescence in situ hybridization technique
Hyung Geun Song,
Seon Ok Choi
and In Beom Kang
Abstract
We report here several experiences of interphase cytogenetics, using fluorescence in situ hybridization (FISH) technique, for the detection of chromosome aberrations. FISH, using alpha satellite specific probes of 18, X, Y chromosomes, was done in interphase nuclei from peripheral blood of patients with Edwards' syndrome, Klinefelter's syndrome and Turner's syndrome with healthy male and female controls, respectively. The distributions of fluorescent signals in 100 interphase nuclei were well correlated with metaphase findings. Nowadays FISH plays an increasingly important role in a variety of research areas, including cytogenetics, prenatal diagnosis, tumor biology, gene amplification and gene mapping.
Keywords
Fluorescence in situ hybridization; Interphase cytogenetics; Chromosome aberration
MeSH Terms
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