Araştırma Makalesi
Comparison of parthenogenetic oocyte activation in different mouse strains on in vitro development rate and quality
Öz
The aim of our research is to investigate the effects of parthenogenetic activation on in vitro embryo development rates in different mouse strains. B6CBAF1, C57BL/6j, and B6D2F1 mouse strains were used in this study. Superovulated mice were sacrificed and oocytes were obtained 14 hours after the human chorionic gonadotrophin (hCG) injection and the parthenogenetic activation started 18 hours after hCG injection. The oocytes were activated for 6 hours in 10 mM SrCl2 + 5 μg/mL-1 Cytohalasine B (CB) + 5 nM Trichostatin A (TSA) containing Ca2+ free Chatot Ziomek Brinster (CZB) activation medium. After this, further incubation was performed for two hours in an incubator at 37 °C and 5% CO2 in embryo culturing medium + TSA. Finally, embryos were cultured for 120 hours. Parthenogenetic activation success of the B6D2F1 mouse strain was found to be higher than C57BL/6j and B6CBAF1 strains.
Anahtar Kelimeler
Destekleyen Kurum
TUBITAK - The Scientific and Technological Research Council of Turkey
Proje Numarası
Grant Number: TOVAG 114O638
Teşekkür
The authors gratefully acknowledge the use of the services and facilities of the Koç University Research Center for Translational Medicine (KUTTAM), funded by the Republic of Turkey Ministry of Development. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Ministry of Development. This research was supported by a grant from TUBITAK - The Scientific and Technological Research Council of Turkey (Grant Number: TOVAG 114O638).
Kaynakça
- Cuthbertson KS, Whittingham DG, Cobbold PH (1981): Free Ca2+ increases in exponential phases during mouse oocyte activation. Nature, 294, 754-757.
- Kline D. (1996) Activation of the mouse egg. Theriogenology, 45, 81–90.
- Kishikawa H, Wakayama T, Yanagimachi R (1999): Comparison of oocyte-activating agents for mouse cloning. Cloning, 1, 153–159.
- Campbell KHS (1999): Nuclear equivalence, nuclear transfer, and the cell cycle. Cloning, 1, 3–15.
- De Sousa PA, Dobrinsky JR, Zhu J, Archibald AL, Ainslie A et al (2002): Somatic cell nuclear transfer in the pig: control of pronuclear formation and integration with improved methods for activation and maintenance of pregnancy. Biol Reprod, 66, 642–650.
- Ma SF, Liu XY, Miao DQ, Han ZB, Zhang X et al (2005): Parthenogenetic activation of mouse oocytes by strontium chloride: A search for the best conditions. Theriogenology, 64, 1142–1157. Versieren K, Heindryckx B, Lierman S, Gerris J, De Sutter P (2010): Developmental competence of parthenogenetic mouse and human embryos after chemical or electrical activation. Reprod Biomed Online, 21, 769-775.
- Han BS, Gao JL (2013): Effects of chemical combinations on the parthenogenetic activation of mouse oocytes. Exp Ther Med, 5, 1281-1288.
- Bai GY, Song SH, Wang ZD, Shan ZY, Sun RZ et al. (2016): Embryos aggregation improves development and imprinting gene expression in mouse parthenogenesis. Dev Growth Differ, 58, 270-279.
- Fulka H, Hirose M, Inoue K, Ogonuki N, Wakisaka N et al. (2011): Production of mouse embryonic stem cell lines from maturing oocytes by direct conversion of meiosis into mitosis. Stem Cells, 29, 517-527.
- Didié M, Christalla P, Rubart M, Muppala V, Döker S et al. (2013): Parthenogenetic stem cells for tissue-engineered heart repair. J Clin Invest, 123, 1285-1298.
- Daughtry B, Mitalipov S (2014): Concise review: parthenote stem cells for regenerative medicine: genetic, epigenetic, and developmental features. Stem Cells Transl Med, 3, 290-298.
- Dandekar PV, Glass RH (1987): Development of mouse embryos in vitro is affected by strain and culture medium. Gamete Res, 17, 279–285.
- Herrick JR, Paik T, Strauss KJ, Schoolcraft WB, Krisher RL (2016): Building a better mouse embryo assay: effects of mouse strain and in vitro maturation on sensitivity to contaminants of the culture environment. J Assist Reprod Genet, 33, 237-245.
- Czechanski A, Byers C, Greenstein I, Schrode N, Donahue LR et al. (2014): Derivation and characterization of mouse embryonic stem cells from permissive and nonpermissive strains. Nat Protoc, 9, 559-574.
- Taşkın AC, Akkoç T, Sağırkaya H, Bağış H, Arat S (2016): Comparison of the development of mouse embryos manipulated with different biopsy techniques. Turk J Vet Anim Sci, 40, 157-162.
- Taşkın AC, Kocabay A, Ebrahimi A, Karahüseyinoğlu S, Şahin GN et al. (2019): Leptin treatment of in vitro cultured embryos increases outgrowth rate of inner cell mass during embryonic stem cell derivation. In Vitro Cell Dev Biol Anim, 55, 473-481.
- Taşkın AC, Kocabay A (2019): Leptin supplementation in embryo culture medium increases in vivo implantation rates in mice. Turk J Vet Anim Sci, 43, 359-363.
- Mallol A, Santaló J, Ibáñez E (2014): Psammaplin a improves development and quality of somatic cell nuclear transfer mouse embryos. Cellular Reprogram, 16, 392-406.
- Mallol A, Santaló J, Ibáñez E (2013) Comparison of three differential mouse blastocyst staining methods. Syst Biol Reprod Med, 59, 117-122.
- Wakayama T, Perry AC, Zuccotti M, Johnson KR, Yanagimachi R (1998): Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature, 394, 369-374.
- Beck JA, Lloyd S, Hafezparast M, Lennon Pierce M, Eppig JT et al. (2000): Genealogies of mouse inbred strains. Nat Genet, 24, 23–25.
- Heytens E, Soleimani R, Lierman S, De Meester S, Gerris J et al. (2008): Effect of ionomycin on oocyte activation and embryo development in mouse. Reprod Biomed Online, 17, 764-771.
- Sung LY, Chang CC, Amano T, Lin CJ, Amano M et al. (2010): Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes. Cellular Reprogram, 12, 203-211.
- Gao W, Yu X, Hao J, Wang L, Qi M et al. (2019): Ascorbic acid improves parthenogenetic embryo development through TET proteins in mice. Biosci Rep, 39, 1-8.
Farklı fare ırklarında parthenogenotik oosit aktivasyonun in vitro gelişim oran ve kalitesinin karşılaştırılması
Öz
Çalışmamızın amacı, partenogenetik aktivasyonda farklı fare ırklarında in vitro embriyo gelişimi ve kalitesi üzerindeki etkilerinin araştırılmasıdır. Bu çalışmada, B6CBAF1, C57BL/6j, and B6D2F1 farelerin superovulasyon ile elde edilen oositleri kullanılmıştır. Superovule edilen fareler, insan koryonik gonadotropin (hCG) uygulamasından 14 saat sonra oositler elde edildi ve 18 saat sonra partenogenetik aktivasyona başlandı. Oositler, 10 mM SrCl2 + 5 μg/mL-1 sitokalazin B (CB) + 5 nM trikostatin A (TSA) Ca 2+ içermeyen Chatot Ziomek Brinster (CZB) medyumu içerisinde 6 saat bekletildi. Aktivasyon sonrası, embriyo kültür medyumu + TSA’da inkübatörde 37°C ve %5 CO2 ortamında 2 saat bekletildi. Son olarak, tüm embriyolar 120 saat süre ile kültüre edildi. Bu çalışmadan elde edilen sonuçlar göre, B6D2F1 ırkının partenogenetik aktivasyon başarısı, C57BL/6j ve B6CBAF1 ırklarına göre daha yüksek bulundu.
Anahtar Kelimeler
Proje Numarası
Grant Number: TOVAG 114O638
Kaynakça
- Cuthbertson KS, Whittingham DG, Cobbold PH (1981): Free Ca2+ increases in exponential phases during mouse oocyte activation. Nature, 294, 754-757.
- Kline D. (1996) Activation of the mouse egg. Theriogenology, 45, 81–90.
- Kishikawa H, Wakayama T, Yanagimachi R (1999): Comparison of oocyte-activating agents for mouse cloning. Cloning, 1, 153–159.
- Campbell KHS (1999): Nuclear equivalence, nuclear transfer, and the cell cycle. Cloning, 1, 3–15.
- De Sousa PA, Dobrinsky JR, Zhu J, Archibald AL, Ainslie A et al (2002): Somatic cell nuclear transfer in the pig: control of pronuclear formation and integration with improved methods for activation and maintenance of pregnancy. Biol Reprod, 66, 642–650.
- Ma SF, Liu XY, Miao DQ, Han ZB, Zhang X et al (2005): Parthenogenetic activation of mouse oocytes by strontium chloride: A search for the best conditions. Theriogenology, 64, 1142–1157. Versieren K, Heindryckx B, Lierman S, Gerris J, De Sutter P (2010): Developmental competence of parthenogenetic mouse and human embryos after chemical or electrical activation. Reprod Biomed Online, 21, 769-775.
- Han BS, Gao JL (2013): Effects of chemical combinations on the parthenogenetic activation of mouse oocytes. Exp Ther Med, 5, 1281-1288.
- Bai GY, Song SH, Wang ZD, Shan ZY, Sun RZ et al. (2016): Embryos aggregation improves development and imprinting gene expression in mouse parthenogenesis. Dev Growth Differ, 58, 270-279.
- Fulka H, Hirose M, Inoue K, Ogonuki N, Wakisaka N et al. (2011): Production of mouse embryonic stem cell lines from maturing oocytes by direct conversion of meiosis into mitosis. Stem Cells, 29, 517-527.
- Didié M, Christalla P, Rubart M, Muppala V, Döker S et al. (2013): Parthenogenetic stem cells for tissue-engineered heart repair. J Clin Invest, 123, 1285-1298.
- Daughtry B, Mitalipov S (2014): Concise review: parthenote stem cells for regenerative medicine: genetic, epigenetic, and developmental features. Stem Cells Transl Med, 3, 290-298.
- Dandekar PV, Glass RH (1987): Development of mouse embryos in vitro is affected by strain and culture medium. Gamete Res, 17, 279–285.
- Herrick JR, Paik T, Strauss KJ, Schoolcraft WB, Krisher RL (2016): Building a better mouse embryo assay: effects of mouse strain and in vitro maturation on sensitivity to contaminants of the culture environment. J Assist Reprod Genet, 33, 237-245.
- Czechanski A, Byers C, Greenstein I, Schrode N, Donahue LR et al. (2014): Derivation and characterization of mouse embryonic stem cells from permissive and nonpermissive strains. Nat Protoc, 9, 559-574.
- Taşkın AC, Akkoç T, Sağırkaya H, Bağış H, Arat S (2016): Comparison of the development of mouse embryos manipulated with different biopsy techniques. Turk J Vet Anim Sci, 40, 157-162.
- Taşkın AC, Kocabay A, Ebrahimi A, Karahüseyinoğlu S, Şahin GN et al. (2019): Leptin treatment of in vitro cultured embryos increases outgrowth rate of inner cell mass during embryonic stem cell derivation. In Vitro Cell Dev Biol Anim, 55, 473-481.
- Taşkın AC, Kocabay A (2019): Leptin supplementation in embryo culture medium increases in vivo implantation rates in mice. Turk J Vet Anim Sci, 43, 359-363.
- Mallol A, Santaló J, Ibáñez E (2014): Psammaplin a improves development and quality of somatic cell nuclear transfer mouse embryos. Cellular Reprogram, 16, 392-406.
- Mallol A, Santaló J, Ibáñez E (2013) Comparison of three differential mouse blastocyst staining methods. Syst Biol Reprod Med, 59, 117-122.
- Wakayama T, Perry AC, Zuccotti M, Johnson KR, Yanagimachi R (1998): Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature, 394, 369-374.
- Beck JA, Lloyd S, Hafezparast M, Lennon Pierce M, Eppig JT et al. (2000): Genealogies of mouse inbred strains. Nat Genet, 24, 23–25.
- Heytens E, Soleimani R, Lierman S, De Meester S, Gerris J et al. (2008): Effect of ionomycin on oocyte activation and embryo development in mouse. Reprod Biomed Online, 17, 764-771.
- Sung LY, Chang CC, Amano T, Lin CJ, Amano M et al. (2010): Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes. Cellular Reprogram, 12, 203-211.
- Gao W, Yu X, Hao J, Wang L, Qi M et al. (2019): Ascorbic acid improves parthenogenetic embryo development through TET proteins in mice. Biosci Rep, 39, 1-8.
Toplam 24 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Proje Numarası | Grant Number: TOVAG 114O638 |
| Yayımlanma Tarihi | 15 Ocak 2021 |
| Gönderilme Tarihi | 2 Eylül 2020 |
| Kabul Tarihi | 29 Kasım 2020 |
| Yayımlandığı Sayı | Yıl 2021 Cilt: 92 Sayı: 1 |
Veteriner Hekimler Derneği Dergisi açık erişimli bir dergi olup, derginin yayın modeli Budapeşte Erişim Girişimi (BOAI) bildirisine dayanmaktadır. Yayınlanan tüm içerik, çevrimiçi ve ücretsiz olarak sunulan Creative Commons CC BY-NC 4.0 lisansı altında lisanslanmıştır. Yazarlar, Veteriner Hekimler Derneği Dergisi'nde yayınlanan eserlerinin telif haklarını saklı tutarlar.
Veteriner Hekimler Derneği / Turkish Veterinary Medical Society