Photosynthetica 2020, 58(3):702-711 | DOI: 10.32615/ps.2020.022

Transcription analysis of chlorophyll biosynthesis in wildtype and chlorophyll b-lacking rice (Oryza sativa L.)

M.K. NGUYEN1,2,3,5, T.H. SHIH1, S.H. LIN1, W.D HUANG6, C.M. YANG1,5
1 Biodiversity Research Center, Academia Sinica, Nangang, Taipei 115, Taiwan
2 Biodiversity Program, Taiwan International Graduate Program, Biodiversity Research Center, Academia Sinica and National Taiwan Normal University, Taipei 115, Taiwan
3 Department of Life Science, National Taiwan Normal University, Daan, Taipei 115, Taiwan
5 Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh, 70000, Vietnam
6 Department of Agronomy, National Taiwan University, Daan, Taipei 106, Taiwan

The aim of the present study was to investigate the photosynthetic properties and transcriptomic profiles of wildtype and chlorophyll (Chl) b-lacking rice (Oryza sativa L.). The plastid ultrastructure of the Chl b-lacking rice (i.e., loss of starch granules, abundant vesicles, and abundant plastoglobuli) indicated abnormal plastid development, whereas the analysis of transcriptome profiles and differentially expressed genes revealed that gene encoding PsbR (PSII core protein) was downregulated in the mutant, thereby reducing the Chl accumulation of the mutant. Meanwhile, in regards to Chl biosynthesis and degradation pathways, GluTR gene was downregulated, whereas UROD, CPOX, and MgCH genes were upregulated. The qPCR results were generally consistent with those of the transcription analysis, except for the finding that NOL genes, which regulate Chl b degradation, were upregulated. These results suggest that both the reduction in Chl accumulation and increase in conversion rate of Chl b to Chl a caused Chl a/b ratio amplification in mutant. The present study also provides evidence for Chl b degradation via pheophorbide b.

Additional key words: grana; next-generation sequencing; photosynthesis; RNA-Seq; transcriptome.

Received: September 11, 2019; Revised: December 9, 2019; Accepted: March 4, 2020; Prepublished online: April 18, 2020; Published: June 11, 2020  Show citation

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NGUYEN, M.K., SHIH, T.H., LIN, S.H., HUANG, W.D., & YANG, C.M. (2020). Transcription analysis of chlorophyll biosynthesis in wildtype and chlorophyll b-lacking rice (Oryza sativa L.). Photosynthetica, 58(3), 702-711. doi: 10.32615/ps.2020.022
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