biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 63:247-252, 2019 | DOI: 10.32615/bp.2019.028

Application of sucrose modulates the expressions of genes involved in proline and polyamine metabolism in maize seedlings exposed to drought

C. Altuntaº1, A. Sezgin1, M. Demiralay2, R. Terzi1,*, A. Sağlam3, A. Kadioğlu1
1 Karadeniz Technical University, Faculty of Science, Department of Biology, 61080, Trabzon, Turkey
2 Artvin Coruh University, Faculty of Forestry, Departmant of Forest Engineering, 0800, Artvin, Turkey
3 Karadeniz Technical University, Department of Molecular Biology and Genetics, 61080 Trabzon, Turkey

Sucrose, proline, and polyamines are compatible solutes accumulating in plant tissues and increasing cellular osmolarity under environmental stresses. These compatible solutes and hydrogen peroxide can function as signaling molecules in plants. There has been very little evidence how the supply of sucrose changes the biosynthesis of compatible solutes. This study aimed to assess the cross-talk among sucrose, H2O2, and compatible solutes on the expression of genes encoding key enzymes in the pathways of proline and polyamine metabolism in drought stressed maize seedlings. Drought stress (induced by polyethylene glycol solution) increased the expressions of genes encoding pyrroline-5-carboxylate synthetase (P5CS), arginine decarboxylase (ADC), and S-adenosylmethionine decarboxylase (SAMDC), while decreased proline dehydrogenase (ProDH), diamine oxidase (DAO), and polyamine oxidase (PAO) expressions. Addition of sucrose to the stressed seedlings increased the P5CS, ADC and SAMDC expressions more than drought stress alone and reduced more the ProDH, DAO, and PAO expressions. Moreover, exogenous sucrose increased leaf water potential and the content of proline, polyamines, and total soluble sugars, whereas decreased H2O2 content and membrane damages under the drought stress conditions. Consequently, exogenous sucrose contributed to the preservation of water status and the amelioration of damage in maize seedlings under the drought stress.

Keywords: arginine decarboxylase, diamine oxidase, hydrogen peroxide, polyamine oxidase, proline dehydrogenase, pyrroline-5-carboxylate synthetase, S-adenosylmethionine decarboxylase

Accepted: December 8, 2018; Prepublished online: December 8, 2018; Published online: January 19, 2019  Show citation

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Altuntaº, C., Sezgin, A., Demiralay, M., Terzi, R., Sağlam, A., & Kadioğlu, A. (2019). Application of sucrose modulates the expressions of genes involved in proline and polyamine metabolism in maize seedlings exposed to drought. Biologia plantarum, 63, Article 247-252. https://doi.org/10.32615/bp.2019.028
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