We performed a field experiment on throughfall, stemflow, and bamboo culm flow to estimate interception in a deciduous broadleaf forest with different stand structures by separately removing the overstory and understory vegetation. The study area is occupied by oak (Quercus serrata) and chestnut (Castanea crenata) with an understory of chino bamboo (Pleioblastus chino). We established three plots for vegetation control, including an overstory plot (removal of understory), a bamboo plot (removal of overstory), and a control plot (both overstory and understory remained). Throughfall amounts relative to precipitation were 61% in the control plot, 54% in the overstory plot, and 31% in the bamboo plot. Average stemflow in control and overstory plots was 3% of precipitation. The significant difference in throughfall for the bamboo plot may have been caused by the high density of understory vegetation. A large portion of intercepted water is transferred to the ground as bamboo culm flow in the understory beneath the canopy in the control plot and in the bamboo plot. Our experiment highlighted the significance of understory vegetation in altering hydrological processes from canopy to understory vegetation.