Abstract
Although microbial contaminations of domestic appliances are believed to gain importance, especially due to the trend towards lower cleaning temperatures, little is known about the existing microbial communities inside household washing machines. This study aimed to improve the knowledge of microbial composition of biofilms within household washing machines using a cultural-independent technology. Biofilms inside the detergent drawer and the rubber door seal of household washing machines were analysed using the 454-pyrosequencing method. The microbial community inside washing machines appeared to be highly diverse with Proteobacteria as the main prokaryotic and Basidiomycota and Ascomycota as the main fungal colonizers.
Kurzfassung
Obwohl die mikrobielle Kolonisation von Haushaltsgeräten, insbesondere aufgrund des anhaltenden Trends hin zu niedrigen Wasch- und Reinigungstemperaturen, an Bedeutung gewinnen dürfte, ist wenig über die mikrobiellen Gemeinschaften in Haushaltswaschmaschinen bekannt. Im Rahmen dieser Studie wurden die mikrobiellen Gemeinschaften in der Einspülkammer und der Türdichtung von Waschmaschinen mittels 454-Pyrosequencing analysiert, wobei Proteobakterien als die dominanten prokaryotischen Besiedler ermittelt wurden, während Basidiomycota und Ascomycota die am häufigsten gefundenen Pilze innerhalb einer hoch diversen Gemeinschaft darstellten.
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