The Complete Amino Acid Sequence of Human Serum Retinol-binding Protein

  • Lars Rask
  • Helena Anundi
  • Jan Fohlman
  • Per A. Peterson

Abstract

The complete amino acid sequence of human serum Retinol-binding protein (RBP) including the distribution of its three disulfide bridges, has been determined. The protein consists of 182 amino acid residues, the order of which was determined following the isolation of five CNBr-fragments. Direct amino acid sequence analysis in an automatic liquid phase sequencer provided almost the entire sequences of the five CNBr-fragments. Several sets of enzymatically derived peptides of RBP were also used to elucidate the primary structure. RBP displays significant homology to bovine β-lactoglobulin, human α1-microglobulin and rat α1-microglobulin. RBP contains an internal homology. Thus, residues 36 to 83 display statistically significant homology with residues 96 to 141.

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Published
1987-09-01
How to Cite
Rask L., Anundi H., Fohlman J., & Peterson P. A. (1987). The Complete Amino Acid Sequence of Human Serum Retinol-binding Protein. Upsala Journal of Medical Sciences, 92(2), 115-146. https://doi.org/10.3109/03009738709178685
Section
Original Articles