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Regeneration and micropropagation of lemon cultivars in vitro from nodal explants

  • Plant Industry
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Abstract

The effect of sterilization agents and the composition of the nutrient medium on lemon explants regeneration, micropropagation and rooting was investigated. Nodal segments of eight year old lemon trees cv. Novoaphonsky, Beskolyuchy, Udarnik, Meyer lemon were taken as explants during spring. Veltolen 0.2%, 0.2% HgOCl and hypochlorite Ca 7% solutions were used as disinfectants at different exposures. The best results for surface sterilization was obtained when shoots were deeped in Veltolen solution by about 40 min. In this case 58.7% sterile explants was reseived. Several combinations of BAP with NAA were used for optimization of cell proliferation and micropropagation. Maximum effect at the stage of proliferation was obtained by adding to the MS basal medium 0.1 mg/l BAP and 0.5 mg/l NAA Cultural media consisted of MS minerals and vitamins with 1 mg/l BAP and 0.1 mg/l NAA was stimulated multiplication of regenerated plants. Rooting of regenerants was obtained on 1/2 MS supplemented with 0.5 mg/l NAA alone.

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References

  1. Avenido, R.A., et al., Developing Plant Regeneration System for in vitro Conservation of Mandarin and Pumello (International Symposium on Harnessing the Potential of Horticulture in the Asian-Pacific Region), 1994.

  2. Abdulaziz M. Al-Bahrany, Effect of Phytohormones on in vitro Shoot Multiplication and Rooting of Lime Citrus Aurantifolia (Christm.) Swing, Department of Horticulture, College of Agriculture and Food Sciences, King Faisal University, Saudi Arabia, October 2001.

    Google Scholar 

  3. Butenko, R.G., Plant Cell Culture and Biotechnology, Moscow: Nauka, 1986, pp. 111–114.

    Google Scholar 

  4. Carimi, F., Somatic Embryogenesis and Organogenesis in Citrus for Sanitation and in vitro Conservation, Options Méditerranéennes, Série B, no. 33.

  5. Gentile, A., Tribulato, E., Deng, Z.N., and Vardi, A., In Vitro Flovering from Citrus Limon Lateral Buds, J. Plant Physiol., 1990, vol. 133, no. 1, pp. 56–60.

    Google Scholar 

  6. Gentile, A. and Tribulato, E., An Investigation on the Different Plant Growth Regulating Compounds in vitro Shoot Tip and Node Culture of Lemon Seeding, Sc. Hortic., 2001, vol. 89, no. 2, pp. 115–128.

    Article  Google Scholar 

  7. Gentile, A., Tribulato, E., Deng, Z.N., and Vardi, A., In Vitro Selection of Nucellar Lemon Callus and Regeneration of Plants Tolerant to Phoma tracheiphila Toxin, Adv. Hort. Sci., 1992, Vol. 6.

  8. Gmitter, F.G., Grosser, J.W., and Moore, G.A., Citrus, in Biotechnology of Perennial Fruit Crops, Litz, R.E. and Hammerschlag, F., Eds., Oxon: CAB International, 1992, pp. 335–369.

    Google Scholar 

  9. Grosser, J.W., Chandler, J.L., et al., In-Vitro Propagation of Seedless “Cohen” Citrange, Proc. Fla. State Hort. Soc., 1993, vol. 106, pp. 57–60.

    Google Scholar 

  10. Hassanein, A.M. and Azooz, M.M., Propagation of C. reticulata via in vitro Seed Germination and Shoot Cuttings, Biologia Plantarum, 2003/4, vol. 47,issue 2, pp. 173–177.

    Google Scholar 

  11. Jonard, R., Lukman, D., Schall, F., and Villemur, P., In Vitro Culture of Lemon Juice Vesicles, Plant Cell Tissue Organ Cult, 1987, vol. 11, no. 2, pp. 81–95.

    Article  Google Scholar 

  12. Kahn, T., et al., Cooperation in the Conservation of Citrus Genetic Resources: Riverside, California (International Society of Citriculture Proceedings Simposium), April, 2004.

  13. Kuperman, F.M., Plant morphophisiology, Moscow: Vysshaya shkola, 1984, pp. 39–77.

    Google Scholar 

  14. Murashige, T. and Skoog, F., A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures, Physiol. Plant., 1962, vol. 15, pp. 473–497.

    Article  CAS  Google Scholar 

  15. Pausheva, Z.P., Plant Cytology Practice, Moscow: Kolos, 1970, p. 255.

    Google Scholar 

  16. Pérez-Tornero, O., Tallón C.I., and Porras, I., An Efficient Protocol for Micropropagation of Lemon (Citrus limon) from Mature Nodal Segments, Plant Cell Tiss. Organ. Cult, 2010, vol. 100, pp. 263–271.

    Article  Google Scholar 

  17. Paudial, K.P. and Haq, N., In Vitro Propagation of Pummelo (Citrus Grandis L. Osbeck), In vitro Cell. Dev. Biol. DPlant, November–December 2000, vol. 36, pp. 511–516.

    Article  Google Scholar 

  18. Rathore, J.S., Rathore, M.S., and Singh, M., Micropropagation of Mature Tree of Citrus Limon, Indian Journal of Biotechnology, April 2007, vol. 6, pp. 239–244.

    CAS  Google Scholar 

  19. Shengeliya, L.N. and Kataeva, N.V., Stem Nodes Culture of Citrus spp, Kishinev: Kultura kletok rastenii I biotechnologiya, 1983, p. 230.

    Google Scholar 

  20. Shengeliya, L.N. and Butenko, R.G., Micropropagation of Citrus in vitro, Kishinev: Kultura kletok rastenii I biotechnologiya, 1983, pp. 116, 117.

    Google Scholar 

  21. Usman, M., Sana, M., and Fatima, B., In Vitro Multiple Shoot Induction from Nodal Explants of Citrus Cultivars, Central European Agricult. Journ., 2005, vol. 6, no. 4, pp. 435–442.

    Google Scholar 

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Authors and Affiliations

  1. All-Russia Scientific Institute of Floriculture and Subtropical Crops of Russian Academy of Agricultural Science, 354064, Sochi, Russia

    L. S. Samarina, T. M. Kolomiets & E. S. Arutyunova

  2. All-Russia Scientific Institute of Agricultural Biotechnology of Russian Academy of A Agricultural Science, Moscow, Russia

    E. N. Baranova

Authors
  1. L. S. Samarina
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  2. T. M. Kolomiets
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  3. E. N. Baranova
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  4. E. S. Arutyunova
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Correspondence to L. S. Samarina.

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The text was submitted by authors in English.

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Samarina, L.S., Kolomiets, T.M., Baranova, E.N. et al. Regeneration and micropropagation of lemon cultivars in vitro from nodal explants. Russ. Agricult. Sci. 36, 417–420 (2010). https://doi.org/10.3103/S106836741006008X

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  • DOI: https://doi.org/10.3103/S106836741006008X

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