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EXPANSION OF THE EXPERIMENTAL FACILITY AND DEVELOPMENT OF A TECHNIQUE FOR IRRADIATING CELL CULTURES, BASED ON THE PROTON THERAPY COMPLEX PROMETHEUS

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An Erratum to this article was published on 01 February 2023

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Abstract—

Works on the optimization of the experimental stand and the development of a technique for analyzing the biological effects of irradiation of cell cultures with a scanning proton beam of the therapeutic energy range are shown. The results of studies of the effect of proton radiation on human adenocarcinoma tumor cells of the MCF-7 line are presented. Within the study, the optimal radiation dose, exposure time after irradiation, cell culture seeding density, and viability assessment technique are selected. The proton therapy complex Prometheus is used as a radiation source. It is found that the MTT assay method is not suitable for the analysis of the biological effects of proton beam exposure. The analysis of clonogenic activity provides the most reproducible and statistically significant results.

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Funding

This study was supported within the Federal Scientific and Technical Program no. 075-15-2021-1347 (October 5, 2021) of the Ministry of Science and Higher Education of the Russian Federation “Development of new technologies of diagnostics and beam therapy of socially diseases by proton and ion beams using the binary nuclear methods.”

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Authors and Affiliations

  1. Lebedev Physical Institute, Russian Academy of Sciences, 119991, Moscow, Russia

    I. N. Zavestovskaya, A. E. Shemyakov, A. A. Pryanichnikov, M. A. Belikhin, D. S. Petrunya & A. L. Popov

  2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia

    D. D. Kolmanovich & A. L. Popov

  3. Lomonosov Moscow State University, 119991, Moscow, Russia

    A. A. Pryanichnikov & M. A. Belikhin

Authors
  1. I. N. Zavestovskaya
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  2. A. E. Shemyakov
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  3. A. A. Pryanichnikov
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  4. D. D. Kolmanovich
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  5. M. A. Belikhin
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  6. D. S. Petrunya
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  7. A. L. Popov
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Corresponding author

Correspondence to A. A. Pryanichnikov.

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The authors declare that they have no conflicts of interest.

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Translated by A. Kazantsev

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Zavestovskaya, I.N., Shemyakov, A.E., Pryanichnikov, A.A. et al. EXPANSION OF THE EXPERIMENTAL FACILITY AND DEVELOPMENT OF A TECHNIQUE FOR IRRADIATING CELL CULTURES, BASED ON THE PROTON THERAPY COMPLEX PROMETHEUS. Bull. Lebedev Phys. Inst. 49, 145–150 (2022). https://doi.org/10.3103/S1068335622050050

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