Abstract
This work describes the results of analysis in silico of the genetic diversity of the citrulline ureidase gene (ctu) in two species of bacteria of the genus Francisella: tularensis (ssp. tularensis, holarctica, mediasiatica, novicida) and philomiragia. The strains of the Central Asiatic subspecies possessing citrulline ureidase activity differ in the ctu gene from ssp. tularensis Schu by three nucleotide substitutions leading to two insignificant amino acid substitutions in the encoded polypeptide. In the strain of F. tularensis ssp. holarctica, the ctu gene encodes an inactive enzyme, which is probably due to amino acid substitutions 151Gly → Asp, 183Pro → Leu, and 222Asp → Asn. Except for the Japan biovar bacteria, all strains of the Holarctic subspecies contain two stop codons in the ctu gene. The bacteria of the novicida subspecies contain the ctu gene only in the strain 3523, whereas the other strains contain the FTN_0827 gene encoding the CN hydrolase, which probably provides the citrulline ureidase activity.
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References
Bergey’s Manual of Systematic Bacteriology, Garrity, G., Ed., New York: Springer, 2005, vol. 2.
Champion, M.D., Zeng, Q., Nix, E.B., Nano, F.E., Keim, P., Kodira, C.D., et al., Comparative genomic characterization of Francisella tularensis strains belonging to low and high virulence subspecies, PLoS Pathog., 2009, vol. 5, no. 5, p. e1000459.
Laboratornaya diagnostika opasnykh infektsionnykh boleznei: Prakticheskoe rukovodstvo (Laboratory Diagnosis of Dangerous Infectious Diseases. Handbook), Onishchenko, G.G. and Kutyrev, V.V., Eds., Moscow: Zakrytoe Aktsion. Obshch. Shiko, 2013.
Fleming, D.E. and Foshay, L., Studies on the physiology of virulence of Pasteurella tularensis. I. Citrulline ureidase and deamidase activity, J. Bacteriol., 1955, vol. 70, no. 3, pp. 345–349.
Fleming, D.E. and Foshay, L., Studies on the physiology of virulence of Pasteurella tularensis. II. Serine deaminase and transaminase activity, J. Bacteriol., 1956, vol. 71, no. 3, pp. 324–327.
Rodionova, I.V., Citrulline ureidase activity in geographical races of Francisella tularensis, Dokl. Akad. Nauk SSSR, 1968, vol. 179, no. 2, pp. 457–460.
Rodionova, I.V., Differentiation of Francisella tularensis geographical races based on citrulline ureidase activity, Labor. Delo, 1970, vol. 1, pp. 42–43.
Clarridge, J.E., Raich, T.J., Sjösted, A., Sandström, G., Darouiche, R.O., Shawar, R.M., et al., Characterization of two unusual clinically significant Francisella strains, J. Clin. Microbiol., 1996, vol. 34, no. 8, pp. 1995–2000.
Siddaramappa, S., Challacombe, J.F., Petersen, J.M., Pillai, S., Hogg, G., and Kuske, C.R., Common ancestry and novel genetic traits of Francisella novicida-like isolates from North America and Australia as revealed by comparative genomic analyses, Appl. Environ. Microbiol., 2011, vol. 77, no. 15, pp. 5110–5122.
Marchette, N.J. and Nicholes, P.S., Virulence and citrulline ureidase activity of Pasteurella tularensis, J. Bacteriol., 1961, vol. 82, pp. 26–32.
Mahawar, M.J., Kirimanjeswara, G.S., Metzger, D.W., and Bakshi, C.S., Contribution of citrulline ureidase to Francisella tularensis strain Schu S4 pathogenesis, J. Bacteriol., 2009, vol. 191, no. 15, pp. 4798–4806.
Wu, G.Y. and Brosnon, J.T., Macrophages can convert citrulline into arginine, Biochemistry, 1992, vol. 28, pp. 145–148.
Sonck, K.A., Kint, G., Schoofs, G., Vander, W.C., Vanderleyden, J., and De Keersmaecker, S.C., The proteome of Salmonella typhimurium grown under in vivo-mimicking conditions, Proteomics, 2009, vol. 95, pp. 65–79.
Larsson, P., Oyston, P.C., Chain, P., Chu, M.C., Duffield, M., Fuxelius, H.H., et al., The complete genome sequence of Francisella tularensis, the causative agent of tularemia, Nat. Genet., 2005, vol. 37, pp. 153–159.
Kudelina, R.I. and Olsuf’ev, N.G., Differentiation of geographical races of tularensis germ with the help of some antibiotics, Zh. Mikrobiol., Epidemiol. Immunobiol., 1973, vol. 4, pp. 3–9.
Svensson, K., Larsson, P., Johansson, D., Byström, M., Forsman, M., and Johansson, A., Evolution of subspecies of Francisella tularensis, J. Bacteriol., 2005, vol. 187, no. 11, pp. 3903–3908.
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Original Russian Text © V.S. Timofeev, I.V. Bakhteeva, V.M. Pavlov, A.N. Mokrievich, 2015, published in Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya, 2015, No. 4, pp. 15–22.
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Timofeev, V.S., Bakhteeva, I.V., Pavlov, V.M. et al. Citrulline ureidase gene diversity in the genus Francisella . Mol. Genet. Microbiol. Virol. 30, 173–180 (2015). https://doi.org/10.3103/S0891416815040126
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DOI: https://doi.org/10.3103/S0891416815040126