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Endocytic pathway of alpha-fetoprotein in mouse bone marrow hematopoietic stem cells: Molecular characterization and role in biological activity modification

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Abstract

The endocytic pathway of alpha-fetoprotein (AFP) in three types of mouse bone marrow hematopoietic stem cells (HSCs) different by the degree of maturity is similar to the natural pathway: it is based on receptor-mediated tranasport through the membrane p60, p65, and p305 AFP receptors with a clathrin-dependent mechanism of receptosome organization, in the formation and generation of which the AP-2 complex, endofilin, and dynamin are involved. In the studied types of HSCs, nongradient AFP was recorded in the Golgi complex, where it appeared by merging with the formed receptosome, as well as in the cytoplasm, endoplasmatic reticulum, and mitochondria, presumably through consequent binding with cytoplasmic carrier proteins (p55, p52, p62, and p67 AFP receptors) and also to transorganelle transporter proteins (p145 and p182 AFP receptors). A study of the role of AFP endocytosis in the modification of the biological activity of the studied HSC types has shown that it directly regulates protein synthesis and metabolic activity, while its effect on other examined types of biological activity was mediated by ERK1/2.

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Authors and Affiliations

  1. Scientific Center for Anti-Infectious Drugs, Auezov street 84, Almaty, 050060, Kazakhstan

    A. Yu. Bogdanov, T. M. Bogdanova & A. I. Ilin

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  1. A. Yu. Bogdanov
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  2. T. M. Bogdanova
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  3. A. I. Ilin
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Correspondence to A. Yu. Bogdanov.

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Original Russian Text © A.Yu. Bogdanov, T.M. Bogdanova, A.I. Ilin, 2014, published in Tsitologiya i Genetika, 2014, Vol. 48, No. 1, pp. 25–40.

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Bogdanov, A.Y., Bogdanova, T.M. & Ilin, A.I. Endocytic pathway of alpha-fetoprotein in mouse bone marrow hematopoietic stem cells: Molecular characterization and role in biological activity modification. Cytol. Genet. 48, 21–32 (2014). https://doi.org/10.3103/S0095452714010034

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  • DOI: https://doi.org/10.3103/S0095452714010034

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