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Radiation-induced modification of human somatic cells’ chromosome sensitivity to the testing mutagenic exposure of bleomycin in vitro in lung cancer patients in delayed terms following chernobyl accident

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Abstract

The above-background level of cytogenetic effect has been studied using the modified G2-bleomycin sensitivity assay, the value of which was a marker of hidden chromosome instability (HCI). The following three groups were examined: (1) Chernobyl disaster liquidators (CDLs) (occupational group I), (2) patients with lung cancer (LC) who denied voluntary contact with ionizing radiation (comparative group); and (3) patients with LC participating in the liquidation of the Chernobyl disaster (occupational group 2). Significant interindividual variations in the cytogenetic effects induced by bleomycin and the absence of a positive correlation with the background and above-background frequency of chromosome aberrations have been revealed for all the three groups. It was established that occupational group 2 experienced the highest level of above-background cytogenetic effect and therefore had the highest number of patients with HCI. The data allowed us to hypothesize the existence of an association between a radiation-induced increase in individual susceptibility to testing mutagenic exposure and the development of cancer pathologies in patients exposed to ionizing radiation. The results showed the feasibility of using the G2-bleomycin sensitivity assay for the examination of irradiated contingents for the detection of HCI as an informative marker of predisposition to cancer.

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Authors and Affiliations

  1. National Research Center for Radiation Medicine, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    M. A. Pilinskaya, S. S. Dibskiy, E. B. Dibskaya & L. I. Shvaiko

Authors
  1. M. A. Pilinskaya
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  2. S. S. Dibskiy
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  3. E. B. Dibskaya
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  4. L. I. Shvaiko
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Correspondence to M. A. Pilinskaya.

Additional information

Original Russian Text © M.A. Pilinskaya, S.S. Dibskiy, E.B. Dibskaya, L.I. Shvaiko, 2012, published in Tsitologiya i Genetika, 2012, Vol. 46, No. 6, pp. 36–43.

The text was submitted by the authors in English.

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Pilinskaya, M.A., Dibskiy, S.S., Dibskaya, E.B. et al. Radiation-induced modification of human somatic cells’ chromosome sensitivity to the testing mutagenic exposure of bleomycin in vitro in lung cancer patients in delayed terms following chernobyl accident. Cytol. Genet. 46, 360–365 (2012). https://doi.org/10.3103/S0095452712060084

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  • DOI: https://doi.org/10.3103/S0095452712060084

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