Skip to main content
Log in

MAST2-like proteinkinase from grape Vitis vinifera: Cloning of catalytic domain cDNA

  • Published:
Cytology and Genetics Aims and scope Submit manuscript

Abstract

During the work on the project on the identification of proteinkinases that phosphorylate protein microtubules of plants, we revealed with the help of bioinformatics the genes of assumed homologues of proteinkinase MAST2 that is associated with microtubules in animal cells. Respectively, the gene of the closest homologue of MAST2, the assumed protein that we have named GMLK (Grape MAST2-Like Kinase, A7NTE9_VITVI) was identified in the genome of grape Vitis vinifera. This study presents the results of the successful cloning of protein GMLK (A7NTE9_VITVI) cDNA from the leaves of the Pinot Noir grape.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Wade, R.H., On and Around Microtubules: An Overview, Mol. Biotechnol., 2009, vol. 43, no. 2, pp. 177–191.

    Article  PubMed  CAS  Google Scholar 

  2. Wasteneys, G.O., Progress in Understanding the Role of Microtubules in Plant Cells, Curr. Opin. Plant Biol., 2004, vol. 7, no. 6, pp. 651–660.

    Article  PubMed  CAS  Google Scholar 

  3. Minc, N., Bratman, S.V., Basu, R., and Chang, F., Establishing New Sites of Polarization by Microtubules, Curr. Biol., 2009, vol. 19, no. 2, pp. 83–94.

    Article  PubMed  CAS  Google Scholar 

  4. Lopus, M., Yenjerla, M., and Wilson, L., Microtubule Dynamics (Advanced Review), in Wiley Encyclopedia of Chemical Biology, Begley, T.P., Ed., NJ: Wiley, 2009, vol. 3, pp. 153–160.

    Google Scholar 

  5. Hammond, J.W., Cai, D., and Verhey, K.J., Tubulin Modifications and Their Cellular Functions, Curr. Opin. Cell Biol., 2008, vol. 20, no. 1, pp. 71–76.

    Article  PubMed  CAS  Google Scholar 

  6. Verhey, K.J. and Gaertig, J., The Tubulin Code, Cell Cycle, 2007, vol. 6, no. 17, pp. 2152–2160.

    PubMed  CAS  Google Scholar 

  7. Westermann, S. and Weber, K., Post-Translational Modifications Regulate Microtubule Function, Nat. Rev. Mol. Cell. Biol., 2003, vol. 4, no. 12, pp. 938–947.

    Article  PubMed  CAS  Google Scholar 

  8. Fourest-Lieuvin, A., Peris, L., Gache, V., et al., Microtubule Regulation in Mitosis: Tubulin Phosphorylation by the Cyclin-Dependent Kinase Cdk1, Mol. Biol. Cell, 2006, vol. 17, no. 3, pp. 1041–1050.

    Article  PubMed  CAS  Google Scholar 

  9. Hong, K.U., Kim, H.J., Kim, H.S., et al., Cdk1-Cyclin B1-Mediated Phosphorylation of Tumor-Associated Microtubule-Associated Protein/Cytoskeleton-Associated Protein 2 in Mitosis, J. Biol. Chem., 2009, vol. 284, no. 24, pp. 16501–16512.

    Article  PubMed  CAS  Google Scholar 

  10. Kufer, T.A., Nigg, E.A., and Sillje, H.H., Regulation of Aurora-A Kinase on the Mitotic Spindle, Chromosoma, 2003, vol. 112, no. 4, pp. 159–163.

    Article  PubMed  CAS  Google Scholar 

  11. Holmfeldt, P., Stenmart, S., and Gullberg, M., Interphase-Specific Phosphorylation-Mediated Regulation of Tubulin Dimmer Partitioning in Human Cells, Mol. Biol. Cell, 2007, vol. 18, no. 5, pp. 1909–1917.

    Article  PubMed  CAS  Google Scholar 

  12. Blume, Ya., Smertenko, A., Ostapets, N.N., Viklicky, V., and Draber, P., Post-Translational Modifications of Plant Tubulin, Cell Biol. Int., 1997, vol. 21, pp. 918–920.

    Google Scholar 

  13. Ben-Nissan, G., Cui, W., Kim, D.-J., et al., Arabidopsis Casein Kinase 1-Like 6 Contains a Microtubule-Binding Domain and Affects the Organization of Cortical Microtubules, Plant. Physiol., 2008, vol. 148, pp. 1897–1907.

    Article  PubMed  CAS  Google Scholar 

  14. Yemets, A.I., Lloyd, C., and Blume, Ya.B., Plant Tubulin Phosphorylation and Its Role in Cell Cycle Progression, in The Plant Cytoskeleton: Key Tool for Agro-Biotechnology, Baird, W.V., Blume, Ya.B., Breviario, D., and Yemets, A.I., Eds., Dordreht: Springer, 2008, pp. 145–159.

    Google Scholar 

  15. Blume, Y., Yemets, A., Sheremet, Y., et al., Exposure of Beta-Tubulin Regions Defined by Antibodies on an Arabidopsis thaliana Microtubule Protofilament Model and in the Cells, BMC Plant Biol., 2010, vol. 10, no. 1, #29 (doi: 10.1186/1471-2229-10-29).

  16. Blume, Ya.B., Yemets, A.I., Sulimenko, V., et al., Tyrosine Phosphorylation of Tubulin in Plant Cells, Planta, 2008, vol. 229, no. 1, pp. 143–150.

    Article  PubMed  CAS  Google Scholar 

  17. Liu, B.-Q., Jin, L., Zhu, L., Li, J., Huang, S., and Yaun, M., Phosphorylation of Microtubule-Associated Protein SB401 from Solanum berthaultii Regulates Its Effect on Microtubules, J. Integr. Plant Biol., 2006, vol. 51, no. 3, pp. 235–242.

    Article  CAS  Google Scholar 

  18. Sasabe, M., Soyano, T., Takahashi, Y., et al., Phosphorylation of NtMAP65-1 by a MAP Kinase Down-Regulates Its Activity of Microtubule Bundling and Stimulates Progression of Cytokinesis of Tobacco Cells, Genes Dev., 2006, vol. 20, pp. 1004–1014.

    Article  PubMed  CAS  Google Scholar 

  19. Walia, A., Lee, J.S., Wasteneys, G., and Ellis, B., Arabidopsis Mitogen-Activated Protein Kinase MPK18 Mediates Cortical Microtubule Functions in Plant Cells, Plant J., 2009, vol. 59, no. 4, pp. 565–575.

    Article  PubMed  CAS  Google Scholar 

  20. Sheremet, Ya.A., Yemets, A.I., Vissenberg, K., Verbelen, J.-P., and Blume, Ya.B., Effect of Serine/Threonine Protein Kinase Inhibitors on the Morphology of Arabidorsis thaliana Root and Microtubule Organization in Its Cells, Tsitologiya, 2010, vol. 52, no. 5, pp. 389–398.

    CAS  Google Scholar 

  21. Yemets, A., Sheremet, Y., Vissenberg, K., Van Orden, J., Verbelen, J.P., and Blume, Y.B., Effects of Tyrosine Kinase and Phosphatase Inhibitors on Microtubules in Arabidopsis Root Cells, Cell. Biol. Int., 2008, vol. 32, no. 6, pp. 630–637.

    Article  PubMed  CAS  Google Scholar 

  22. Karpov, P.A. and Blume, Ya.B., Bioinformatic Search for Plant Homologues of Animal Structural Maps in the Arabidopsis thaliana Genome, in The Plant Cytoskeleton: Key Tool for Agro-Biotechnology, Baird, W.V., Blume, Ya.B., Breviario, D., and Yemets, A.I., Eds., Dordreht: Springer, 2008, pp. 373–394.

    Google Scholar 

  23. Karpov, P.A., Emets, A.I., Matusov, V.G., Nyporko, A.Yu., Nadezhdina, E.S., and Blume, Ya.B., Bioinformatics Search for Plant Homologues of Ste20-like Serine/Threonine Protein Kinases, Tsitol. Genet., 2009, vol. 43, no. 6, pp. 68–77 [Cytol. Genet. (Eng. Transl.), 2009, vol. 43, no. 6, pp. 419–428].

    PubMed  CAS  Google Scholar 

  24. Karpov, P.A., Emets, A.I., Matusov, V.G., Nyporko, A.Yu., Nadezhdina, E.S., Shashina, N.Yu., and Blume, Ya.B., Bioinformational Search for Plant Homologues of Microtubule-Associated Protein Kinase MAST2, Tr. Nikit. Bot. Sada, 2009, vol. 131, pp. 181–187 (http://www.nbuv.gov.ua/portal/Chem_Biol/znpdnbs/2009_131.pdf).

    Google Scholar 

  25. Karpov, P.A., Nadezhdina, E.S., Emets, A.I., Matusov, V.G., Nyporko, N.Yu., Shashina, N.Yu., and Blyum, Ya.B., Bioinformatic Search of Plant Protein Kinases Involved in the Phosphorylation of Microtubular Proteins and the Regulation of the Cell Cycle, Tsitol. Genet., 2009, vol. 43, no. 3, pp. 63–79 [Cytol. Genet. (Eng. Transl.), 2009, vol. 43, no. 3, pp. 201–215].

    PubMed  CAS  Google Scholar 

  26. Karpov, P.A., Nadezhdina, E.S., Yemets, A.I., Matusov, V.G., Nyporko, A.Yu., Shashina, N.Yu.., and Blume, Ya.B., Bioinformatic Search of Plant Microtubule- and Cell Cycle Related Serine-Threonine Protein Kinases, BMC Genome, 2010, vol. 11(Suppl 1), p. 14 (doi: 10.1186/1471-2164-11S1-S14).

    Article  CAS  Google Scholar 

  27. Garland, P., Quraishe, S., French, P., and O’Connor, V., Expression of the MAST Family of Serine/Threonine Kinases, Brain Res., 2008, vol. 1195, pp. 12–19.

    Article  PubMed  CAS  Google Scholar 

  28. Manning, G., Whyte, D.B., Martinez, R., Hunter, T., and Sudarsanam, S., The Protein Kinase Complement of the Human Genome, Science, 2002, vol. 298, pp. 1912–1934.

    Article  PubMed  CAS  Google Scholar 

  29. Moser, C., Gatto, P., Moser, M., Pindo, M., and Velasco, R., Isolation of Functional RNA from Small Amounts of Different Grape and Apple Tissues, Mol. Biotechnol., 2004, vol. 26, no. 2, pp. 295–299.

    Article  Google Scholar 

  30. Gambino, G., Perrone, I., and Gribaudo, I., A Rapid and Effective Method for RNA Extraction from Different Tissues of Grapevine and Other Woody Plants, Phytochem. Anal., 2008, vol. 19, no. 6, pp. 520–525.

    Article  PubMed  CAS  Google Scholar 

  31. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D.J., Gapped BLAST and PSI-BLADT: A New Generation of Protein Database Search Programs, Nucleic Acids Res., 1997, vol. 25, pp. 3389–3402.

    Article  PubMed  CAS  Google Scholar 

  32. Wang, D., Lee, H.J., Cooper, D.S., Cebotaro, L., Walden, P.D., Choi, I., and Yun, C.C., Coexpression of MAST205 Inhibits the Activity of Na+/H+ Exchanger NHE3, Amer. J. Physiol. Renal. Physiol., 2006, vol. 290, no. 2, pp. 428–437.

    Article  CAS  Google Scholar 

  33. Demidov, D., Van Damme, D., Geelen, D., Blattner, F.R., and Houben, A., Identification and Dynamics of Two Classes of Aurora-Like Kinases in Arabidopsis and Other Plants, Plant Cell, 2005, vol. 17, no. 3, pp. 836–848.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Original Russian Text © S.A. Bryantseva, E.S. Gavryushina, A.I. Yemets, P.A. Karpov, Ya.B. Blume, Yu.F. Drygin, E.S. Nadezhdina, 2010, published in Tsitologiya i Genetika, 2010, Vol. 44, No. 4, pp. 41–47.

About this article

Cite this article

Bryantseva, S.A., Gavryushina, E.S., Yemets, A.I. et al. MAST2-like proteinkinase from grape Vitis vinifera: Cloning of catalytic domain cDNA. Cytol. Genet. 44, 227–232 (2010). https://doi.org/10.3103/S0095452710040079

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S0095452710040079

Keywords

Navigation