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Electrochemical Biosensor Based on Nanosized Polymer-Enzyme Films Composed of Linear Poly(N,N-Dimethylaminoethyl Methacrylate) and Choline Oxidase

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Moscow University Chemistry Bulletin Aims and scope

Abstract

Nanosized polymer-enzyme films were fabricated on graphite surfaces via a sequential two-stage adsorption of the pH- and thermoresponsive polymer, viz., linear poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA), and the enzyme, viz., choline oxidase (ChO). The influence of the conditions (pH and temperature), which are applied for deposition of the polymer onto graphite surface, on electrochemical responses of the polymer-enzyme sensor constructs and kinetic parameters of enzymatic oxidation of choline by ChO integrated into the polymer-enzyme films was revealed. Stimuli-sensitivity of PDMAEMA was found to control the properties of the formed polymer coatings (surface coverage, film thickness and roughness), which decisively determine the amount of the ChO bound by the polymer film and, consequently, the resultant electrochemical characteristics of the polymer-enzyme sensor constructs.

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ACKNOWLEDGMENTS

The study was performed using the equipment purchased as part of the Moscow State University's Program of Development.

Funding

This study was financially supported by the State Registration Topics “Molecular Design, Structure-Function Analysis and Regulation of Enzyme Systems, Cellular Structures, Bionanomaterials: Fundamental Basis and Applications in Technology, Medicine, Environmental Protection” for 2021–2023 (no. AAAA-A21-121011290089-4) and “Modern Problems in Chemistry and Physical Chemistry of High Molecular Compounds” for 2021–2023 (no. АААА-А21-121011990022-4).

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, 119991, Moscow, Russia

    L. V. Sigolaeva, A. Yu. Konyakhina, D. V. Pergushov & I. N. Kurochkin

  2. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334, Moscow, Russia

    I. N. Kurochkin

Authors
  1. L. V. Sigolaeva
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  2. A. Yu. Konyakhina
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  3. D. V. Pergushov
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  4. I. N. Kurochkin
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Correspondence to L. V. Sigolaeva.

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Translated by K. Utegenov

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Sigolaeva, L.V., Konyakhina, A.Y., Pergushov, D.V. et al. Electrochemical Biosensor Based on Nanosized Polymer-Enzyme Films Composed of Linear Poly(N,N-Dimethylaminoethyl Methacrylate) and Choline Oxidase. Moscow Univ. Chem. Bull. 76, 334–342 (2021). https://doi.org/10.3103/S0027131421050060

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  • DOI: https://doi.org/10.3103/S0027131421050060

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