Skip to main content
SpringerLink
Log in

Determination of antioxidants by sensitized chemiluminescence using 2,2′-azo-bis(2-amidinopropane)

  • Published:
Moscow University Chemistry Bulletin Aims and scope

Abstract

An analytical procedure for determining antioxidants was proposed based on chemiluminescence. The analytical system consisted of a free-radicals generator, 2,2′-azo-bis(2-amidinopropane), luminol was used as a chemiluminescence sensitizer in phosphate buffer solution (pH 7.4). The analytical conditions were optimized. The analytical procedure detection limits as low as (μM) the following: for trolox, the limit is 0.05; for ascorbate, it is 0.20; for quercetin, it is 0.03; and, for dihydroquercetin, it is 0.03. A test determination of antioxidants in food was performed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Stocker, P., Lesgards, J.F., Vidal, N., Chalier, F., and Prost M., Biochim. Biophys. Acta, 2003, vol. 1621, p. 1.

    Article  CAS  Google Scholar 

  2. Maulik, G., Maulik, N., Bhandari, V., Kagan, V.E., Pakrashi, S., and Das, D.K., Free Rad. Biol. Res., 1997, vol. 27, p. 221.

    Article  CAS  Google Scholar 

  3. Visioli, F., and Galli, C., Anal. Biochem., 1997, vol. 249 p. 244.

  4. Saez, F., Motta, C., Boucher, D., and Grizard, G., Mol. Hum. Reprod., 1998, vol. 4, p. 667.

    Article  CAS  Google Scholar 

  5. Kawagoe, M., and Nakagawa, K., Toxicol. Lett., 2000, vol. 114, p. 189.

    Article  CAS  Google Scholar 

  6. Takami, M., Preston, S.L., and Behrman, H.R., Am. J. Physiol. Cell. Physiol., 2000, vol. 278, p. 646.

    Google Scholar 

  7. Krasowska, A., Piasecki, A., Murzyn, A., and Sigler, K., Folia Microbiol., 2007, vol. 52, p. 45.

    Article  CAS  Google Scholar 

  8. Campos, A.M., Sotomayor, C.P., Pino, E., and Lissi, E., Biol. Res., 2004, vol. 137, p. 287.

    Google Scholar 

  9. Campos, A.M., Escobar, J., and Lissi, E., J. Braz. Chem. Soc., 1996, vol. 7, p. 43.

    CAS  Google Scholar 

  10. Kim, D.O., Lee, K.W., Lee, H.J., and Lee, C.Y., J. Agric. Food Chem., 2002, vol. 50, p. 3713.

    Article  CAS  Google Scholar 

  11. Psotova, J., Zahalkova, J., Hrbac, J., Simanek, V., and Bartek, J., Biomed. Pap. Med. Fac. Univ. Palacky Olomouc Czech Repub., 2001, vol. 145, p. 81.

    CAS  Google Scholar 

  12. Cao, G., Alessio, H.M., and Cutler, R.G., Free Rad. Biol. Med., 1993, vol. 14, p. 303.

    Article  CAS  Google Scholar 

  13. Wu, X., Gu, L., and Holden, J., J. of Food Composition and Analysis, 2004, vol. 17, p. 407.

    Article  CAS  Google Scholar 

  14. Georgetti, S.R., Casagrande, R., Di Mambro, V.M., Azzolini, A.E., and Fonseca, M.J., AAPS Pharm Sci., 2003., vol. 5, p. 20.

    Article  Google Scholar 

  15. Cheng, Z., Yan, G., Li, Y., and Chang, W., Anal. Bioanal. Chem., 2003, vol. 375, p. 37.

    Google Scholar 

  16. Lissi, E., Pascual, C., and Del Castillo M.D., Free Radic. Res. Commun., 1992, vol. 17, p. 299.

    Article  CAS  Google Scholar 

  17. Lissi, E., Salim-Hanna, M., and Pascual, C., Free Rad. Biol. Med., 1995, vol. 18, p. 153.

    Article  CAS  Google Scholar 

  18. Krasawska, A., Rosiak, D., Szkapiak, K., Oswiecimska, M., Witek, S., and Likaszewicz M., Cell. Mol. Biol. Lett., 2001, vol. 6, p. 71.

    Google Scholar 

  19. Slavikova, H., Lojek, A., Hamar, J., Duskova, M., Kubala, M., Vondracek, J., and Ciz, M., Free Rad. Biol. Med., 1998, vol. 25, p. 9.

    Article  CAS  Google Scholar 

  20. Uotila, J.T., Kirkkola, A.L., Rorarius, M., Tuimala, R.J., and Metsa-Ketela, T., Free Rad. Biol. Med., 1994, vol. 16, p. 581.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Basic Medicine, Lomonosov Moscow State University, Moscow, Russia

    A. V. Alekseev, E. V. Proskurnina & Yu. A. Vladimirov

Authors
  1. A. V. Alekseev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. V. Proskurnina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yu. A. Vladimirov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. V. Proskurnina.

Additional information

Original Russian Text © A.V. Alekseev, E.V. Proskurnina, Yu.A. Vladimirov, 2012, published in Vestnik Moskovskogo Universiteta. Khimiya, 2012, No. 3, pp. 187–193.

About this article

Cite this article

Alekseev, A.V., Proskurnina, E.V. & Vladimirov, Y.A. Determination of antioxidants by sensitized chemiluminescence using 2,2′-azo-bis(2-amidinopropane). Moscow Univ. Chem. Bull. 67, 127–132 (2012). https://doi.org/10.3103/S0027131412030029

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S0027131412030029

Keywords

Search

Navigation