Research Article


DOI :10.26650/IstanbulJPharm.2018.180013   IUP :10.26650/IstanbulJPharm.2018.180013    Full Text (PDF)

Evaluation of biorelevant media to investigate the dissolution properties on flurbiprofen and to assess cytotoxicity effects on Caco-2 cell line

Diren Sarısaltık YaşınŞükran YılmazZeynep Şafak Teksin

Biorelevant media are used to simulate the physiological conditions in terms of components, pH, osmolality and buffer capacity of the human stomach and intestine in both fasted and fed states In this study, we aim to apply the biorelevant media to Caco-2 cell lines to investigate the cytotoxicity effects via the cell viability ratio and to compare the solubilizing effects of various dissolution media on a poorly soluble model drug. Flurbiprofen (Biopharmaceutics Classification System, BCS Class 2) was selected as a model drug. In dissolution studies the pH effects were predominant at higher pH values, while bile salt effects were dominant at lower pH values. The preparation method, bile salts and the phospholipids did not show any additional effect on Caco-2 cell viability. In the cytotoxicity test, fed state media caused an additional 10-15% decrease in cell viability, compared to the fasted state. Similar results were obtained when using the blank of these media which did not include the bile salt or phospholipid. From this, it is evident that this decrease resulted from the pH values, not the components. In conclusion, the cytotoxicity assessment showed that all the biorelevant media were compatible with 70-90% of cell viability for at least 24 h, and this ratio might be increased by modifying the pH. 


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APA

Sarısaltık Yaşın, D., Yılmaz, Ş., & Teksin, Z. (2018). Evaluation of biorelevant media to investigate the dissolution properties on flurbiprofen and to assess cytotoxicity effects on Caco-2 cell line. İstanbul Journal of Pharmacy, 48(3), 82-88. https://doi.org/10.26650/IstanbulJPharm.2018.180013


AMA

Sarısaltık Yaşın D, Yılmaz Ş, Teksin Z. Evaluation of biorelevant media to investigate the dissolution properties on flurbiprofen and to assess cytotoxicity effects on Caco-2 cell line. İstanbul Journal of Pharmacy. 2018;48(3):82-88. https://doi.org/10.26650/IstanbulJPharm.2018.180013


ABNT

Sarısaltık Yaşın, D.; Yılmaz, Ş.; Teksin, Z. Evaluation of biorelevant media to investigate the dissolution properties on flurbiprofen and to assess cytotoxicity effects on Caco-2 cell line. İstanbul Journal of Pharmacy, [Publisher Location], v. 48, n. 3, p. 82-88, 2018.


Chicago: Author-Date Style

Sarısaltık Yaşın, Diren, and Şükran Yılmaz and Zeynep Şafak Teksin. 2018. “Evaluation of biorelevant media to investigate the dissolution properties on flurbiprofen and to assess cytotoxicity effects on Caco-2 cell line.” İstanbul Journal of Pharmacy 48, no. 3: 82-88. https://doi.org/10.26650/IstanbulJPharm.2018.180013


Chicago: Humanities Style

Sarısaltık Yaşın, Diren, and Şükran Yılmaz and Zeynep Şafak Teksin. Evaluation of biorelevant media to investigate the dissolution properties on flurbiprofen and to assess cytotoxicity effects on Caco-2 cell line.” İstanbul Journal of Pharmacy 48, no. 3 (Mar. 2024): 82-88. https://doi.org/10.26650/IstanbulJPharm.2018.180013


Harvard: Australian Style

Sarısaltık Yaşın, D & Yılmaz, Ş & Teksin, Z 2018, 'Evaluation of biorelevant media to investigate the dissolution properties on flurbiprofen and to assess cytotoxicity effects on Caco-2 cell line', İstanbul Journal of Pharmacy, vol. 48, no. 3, pp. 82-88, viewed 28 Mar. 2024, https://doi.org/10.26650/IstanbulJPharm.2018.180013


Harvard: Author-Date Style

Sarısaltık Yaşın, D. and Yılmaz, Ş. and Teksin, Z. (2018) ‘Evaluation of biorelevant media to investigate the dissolution properties on flurbiprofen and to assess cytotoxicity effects on Caco-2 cell line’, İstanbul Journal of Pharmacy, 48(3), pp. 82-88. https://doi.org/10.26650/IstanbulJPharm.2018.180013 (28 Mar. 2024).


MLA

Sarısaltık Yaşın, Diren, and Şükran Yılmaz and Zeynep Şafak Teksin. Evaluation of biorelevant media to investigate the dissolution properties on flurbiprofen and to assess cytotoxicity effects on Caco-2 cell line.” İstanbul Journal of Pharmacy, vol. 48, no. 3, 2018, pp. 82-88. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2018.180013


Vancouver

Sarısaltık Yaşın D, Yılmaz Ş, Teksin Z. Evaluation of biorelevant media to investigate the dissolution properties on flurbiprofen and to assess cytotoxicity effects on Caco-2 cell line. İstanbul Journal of Pharmacy [Internet]. 28 Mar. 2024 [cited 28 Mar. 2024];48(3):82-88. Available from: https://doi.org/10.26650/IstanbulJPharm.2018.180013 doi: 10.26650/IstanbulJPharm.2018.180013


ISNAD

Sarısaltık Yaşın, Diren - Yılmaz, Şükran - Teksin, Zeynep Şafak. Evaluation of biorelevant media to investigate the dissolution properties on flurbiprofen and to assess cytotoxicity effects on Caco-2 cell line”. İstanbul Journal of Pharmacy 48/3 (Mar. 2024): 82-88. https://doi.org/10.26650/IstanbulJPharm.2018.180013



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Accepted26.11.2018

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