Characteristics and prognostic significance of circRNA-100876 in patients with colorectal cancer

G.-R. Zhou, D.-P. Huang, Z.-F. Sun, X.-F. Zhang

Department of General Surgery, Putuo People’s Hospital of Tongji University, Shanghai, China. Legandsky@163.com

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• Oncology

OBJECTIVE: The purpose of this study was to explore the correlation between circRNA-100876 and the clinicopathological parameters of patients with colorectal cancer (Cc).

PATIENTS AND METHODS: Quantitative real-time polymerase chain reaction (RT-qPCR) was applied to detect the circRNA-100876 expression in Cc tissues and cell lines. Overall survival analysis was carried out to explore the correlation between circRNA-100876 and the prognosis of Cc patients by Kaplan-Meier method and Log-rank method. Subsequently, Chi-square test was used to investigate the clinical significance of circRNA-100876 in the clinicopathological parameters of Cc patients. Moreover, the expression of circRNA-100876 was inhibited by small interfering RNAs (siRNAs) in loss-of-function assay. Finally, the invasion ability of Cc cells was determined by transwell assay.

RESULTS: The results of this study manifested that circRNA-100876 was abnormally overexpressed in Cc tissues and cell lines, and the high expression of circRNA-100876 was clearly associated with the Clinical stage, T classification and Lymph node metastasis of Cc patients. Besides, Cc patients with high expression worsened overall survival. In addition, it was demonstrated that the inhibition of circRNA-100876 reduced the invasion ability of Cc cells.

CONCLUSIONS: Acting as a tumor promoter, circRNA-100876 might be regarded as a new potential biomarker for the diagnosis and therapy of Cc.

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