Authors:	Alberg, Irina Kramer, Stefan Schinnerer, Meike Hu, Qizhi Seidl, Christine Leps, Christian Drude, Natascha Möckel, Diana Rijcken, Cristianne Lammers, Twan Diken, Mustafa Maskos, Michael Morsbach, Svenja Landfester, Katharina Tenzer, Stefan Barz, Matthias Zentel, Rudolf
Title:	Polymeric nanoparticles with neglectable protein corona
Online publication date:	12-Aug-2021
Year of first publication:	2020
Language:	english
Abstract:	The current understanding of nanoparticle–protein interactions indicates that they rapidly adsorb proteins upon introduction into a living organism. The formed protein corona determines thereafter identity and fate of nanoparticles in the body. The present study evaluates the protein affinity of three core-crosslinked polymeric nanoparticles with long circulation times, differing in the hydrophilic polymer material forming the particle surface, namely poly(N-2-hydroxypropylmethacrylamide) (pHPMA), polysarcosine (pSar), and poly(ethylene glycol) (PEG). This includes the nanotherapeutic CPC634, which is currently in clinical phase II evaluation. To investigate possible protein corona formation, the nanoparticles are incubated in human blood plasma and separated by asymmetrical flow field-flow fractionation (AF4). Notably, light scattering shows no detectable differences in particle size or polydispersity upon incubation with plasma for all nanoparticles, while in gel electrophoresis, minor amounts of proteins can be detected in the particle fraction. Label-free quantitative proteomics is additionally applied to analyze and quantify the composition of the proteins. It proves that some proteins are enriched, but their concentration is significantly less than one protein per particle. Thus, most of the nanoparticles are not associated with any proteins. Therefore, this work underlines that polymeric nanoparticles can be synthesized, for which a protein corona formation does not take place.
DDC:	540 Chemie 540 Chemistry and allied sciences 610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 09 Chemie, Pharmazie u. Geowissensch.
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-6260
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY-NC
Information on rights of use:	https://creativecommons.org/licenses/by-nc/4.0/
Journal:	Small : nano micro 16 18
Pages or article number:	1907574
Publisher:	Wiley-VCH
Publisher place:	Weinheim
Issue date:	2020
ISSN:	1613-6829
Publisher URL:	https://doi.org/10.1002/smll.201907574
Publisher DOI:	10.1002/smll.201907574
Appears in collections:	JGU-Publikationen