Authors:	Brahmer, Alexandra Neuberger, Elmo W. I. Simon, Perikles Krämer-Albers, Eva-Maria
Title:	Considerations for the analysis of small extracellular vesicles in physical exercice
Online publication date:	4-Feb-2021
Year of first publication:	2020
Language:	english
Abstract:	Physical exercise induces acute physiological changes leading to enhanced tissue cross-talk and a liberation of extracellular vesicles (EVs) into the circulation. EVs are cell-derived membranous entities which carry bioactive material, such as proteins and RNA species, and are important mediators of cell-cell-communication. Different types of physical exercise interventions trigger the release of diverse EV subpopulations, which are hypothesized to be involved in physiological adaptation processes leading to health benefits and longevity. Large EVs (“microvesicles” and “microparticles”) are studied frequently in the context of physical exercise using straight forward flow cytometry approaches. However, the analysis of small EVs (sEVs) including exosomes is hampered by the complex composition of blood, confounding the methodology of EV isolation and characterization. This mini review presents a concise overview of the current state of research on sEVs released upon physical exercise (ExerVs), highlighting the technical limits of ExerV analysis. The purity of EV preparations is highly influenced by the co-isolation of non-EV structures in the size range or density of EVs, such as lipoproteins and protein aggregates. Technical constraints associated with EV purification challenge the quantification of distinct ExerV populations, the identification of their cargo, and the investigation of their biological functions. Here, we offer recommendations for the isolation and characterization of ExerVs to minimize the effects of these drawbacks. Technological advances in the ExerV research field will improve understanding of the inter-cellular crosstalk induced by physical exercise leading to health benefits
DDC:	570 Biowissenschaften 570 Life sciences 796 Sport 796 Athletic and outdoor sports and games
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 02 Sozialwiss., Medien u. Sport FB 10 Biologie
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-5623
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/deed.en
Journal:	Frontiers in physiology 11
Pages or article number:	576150
Publisher:	Frontiers Research Foundation
Publisher place:	Lausanne
Issue date:	2020
ISSN:	1664-042X
Publisher URL:	https://doi.org/10.3389/fphys.2020.576150
Publisher DOI:	10.3389/fphys.2020.576150
Appears in collections:	JGU-Publikationen