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Assessment of genetic variability among selected species of Apocynaceae

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Abstract

Family Apocynaceae is an economically important family grown as ornamental plants and many wild species have medicinal uses as well. The aim of the present study was to understand the level and pattern of genetic variability among the selected individuals of Apocynaceae. For this purpose, three species of different genera of Apocynaceae, Thevetia peruviana, Alstonia scholaris and Catharanthus roseus, were collected from Rawalpindi and Quaid-i-Azam University forest, Islamabad. To evaluate the level of polymorphism within the species and members of different species, randomly amplified polymorphic DNA (RAPD) markers were used. A series of OPC RAPD primers were used; only six primers of OPC series gave amplification. Highest genetic variation at interspecific and intraspecific levels was shown by OPC 9 and the lowest polymorphism was observed in OPC 4. The data was analyzed by using software Statistica 5.5. In total 105 monomorphic and 272 polymorphic bands were produced from all primers. Therefore, out of 322 amplified products, 26% were monomorphic and 68% were polymorphic. Low genetic diversification was observed both at intraspecific and interspecific level. At the molecular level Alstonia scholaris and Catharanthus roseus (subfamily Plumerioideae) appeared in a group and Thevetia peruviana (subfamily Rauvolfoideae) formed another group, confirming the classification based on morphological characters.

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Authors and Affiliations

  1. Plant Biochemistry and Molecular Biology Laboratory, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, PK-45320, Pakistan

    Tariq Mahmood, Anna Iqbal & Nazia Nazar

  2. Department of Biotechnology, Quaid-i-Azam University, Islamabad, PK-45320, Pakistan

    Ishrat Naveed & Bilal Haider Abbasi

  3. Department of Biochemistry, University of Arid Agriculture, Rawalpindi, PK-46300, Pakistan

    Syed Muhammad Saqlan Naqvi

Authors
  1. Tariq Mahmood
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  2. Anna Iqbal
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  3. Nazia Nazar
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  4. Ishrat Naveed
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  5. Bilal Haider Abbasi
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  6. Syed Muhammad Saqlan Naqvi
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Correspondence to Tariq Mahmood.

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Mahmood, T., Iqbal, A., Nazar, N. et al. Assessment of genetic variability among selected species of Apocynaceae. Biologia 66, 64–67 (2011). https://doi.org/10.2478/s11756-010-0134-3

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  • DOI: https://doi.org/10.2478/s11756-010-0134-3

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