ORIGINAL ARTICLE   Free access

International Angiology 2018 December;37(6):451-6

DOI: 10.23736/S0392-9590.18.04022-1

Copyright © 2018 EDIZIONI MINERVA MEDICA

language: English

Associations between miR-661, miR-1202, lncRNA-HOTAIR, lncRNA-GAS5 and MMP9 in differentiated M2-macrophages of patients with varicose veins

Azin S. BIRANVAND ¹, Mohsen KHOSRAVI ¹, Golnaz ESFANDIARI ¹, Adeleh POURSALEH ¹, Sayed R. HOSSEINI-FARD ¹, Abdollah AMIRFARHANGI ², Mohammad NAJAFI ^{1, 3} ✉

¹ Cellular and Molecular Research Center, Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran; ² Hazrat-e Rasool Hospital, Iran University of Medical Sciences, Tehran, Iran; ³ Firoozabadi Hospital, Tehran, Iran

BACKGROUND: The venous hypertension is suggested as the main cause of varicose disease. Some mediators and growth factors are known as the responsible of cellular events for the progression of venous perturbations. The aim of this study was to investigate non-coding (nc) RNA and MMP9 expression levels in macrophages differentiated from monocytes of patients with varicose veins.
METHODS: The monocytes were isolated from the whole blood samples by RosetteSep kit and were differentiated to macrophages M2 using M-CSF factor. The based on ncRNA-gene network, lncRNA-GAS5, lncRNA-HOTAIR, miRNA-661, miRNA-1202, and MMP9 were selected. The gene expression levels were measured by RT-qPCR technique.
RESULTS: Data showed that the MMP9 gene expression increased (P=0.003) while the GAS5, miRNA-661, and miRNA-1202 expression levels reduced significantly in the differentiated macrophages of patients (P=0.035, P=0.009, and P=0.015, respectively). Furthermore, the MMP9 gene expression levels were conversely related to the GAS5, HOTAIR, miRNA-661 and miRNA-1202 expression levels.
CONCLUSIONS: The results suggested that the lncRNA-GAS5, miRNA-661, miRNA-1202 and MMP9 are involved in varicose disease.

KEY WORDS: Varicose veins - RNA, long noncoding - MicroRNAs - Macrophages