ORIGINAL ARTICLE   

Journal of Neurosurgical Sciences 2018 June;62(3):239-44

DOI: 10.23736/S0390-5616.16.03793-0

Copyright © 2016 EDIZIONI MINERVA MEDICA

language: English

Interleukin-13 receptor alpha 2 as a marker of poorer prognosis in high-grade astrocytomas

Masahiko WANIBUCHI ^{1, 2} ✉, Yuko KATAOKA-SASAKI ², Masanori SASAKI ², Shinichi OKA ², Yayoi OTSUKA ², Miki YAMAGUCHI ³, Hirofumi OHNISHI ⁴, Shunya OHTAKI ^{1, 2}, Shouhei NOSHIRO ^{1, 2}, Satoshi OOKAWA ^{1, 2}, Takeshi MIKAMI ^{1, 2}, Nobuhiro MIKUNI ¹, Osamu HONMOU ²

¹ Department of Neurosurgery, School of Medicine, Sapporo Medical University, Sapporo, Japan; ² Department of Neural Regenerative Medicine, Research Institute for Frontier Medicine, School of Medicine, Sapporo Medical University, Sapporo, Japan; ³ Department of Molecular Medicine, Research Institute for Frontier Medicine, School of Medicine, Sapporo Medical University, Sapporo, Japan; ⁴ Department of Public Health, School of Medicine, Sapporo Medical University, Sapporo, Japan

BACKGROUND: Interleukin-13 receptor alpha 2 (IL13Rα2) is considered a prognostic marker for gliomas according to the World Health Organization (WHO) grade. However, the expression levels of the marker vary from case to case, even within the same grade. We investigated whether IL13Rα2 could serve as a predictor of poorer prognosis in gliomas.
METHODS: mRNA expression of IL13Rα2 was measured using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) in 52 formalin-fixed paraffin-embedded glioma samples (4 pilocytic astrocytomas, 9 diffuse astrocytomas, 12 anaplastic astrocytomas, and 27 glioblastomas, grade IV). The expression levels were compared with regard to WHO grade, MIB-1 index, patient’s age, and overall survival.
RESULTS: Real time qRT-PCR showed that IL13Rα2 is expressed in a subset of cases with a progressive increase from low- to high-grade astrocytomas (HGAs). The expression had a significant positive correlation with the MIB-1 index and advanced patient age at diagnosis. The overall survival (OS) of patients who had HGAs with higher levels of IL13Rα2 expression was significantly lower than the OS of those with HGAs with lower levels of IL13Rα2. In the 39 HGA cases studied, the median survival benefit in the lower expression group was 167.4 months. The median OS (mOS) in HGA group with lower IL13Rα2 expression was 186.4 months, while the mOS in the group with higher IL13Rα2 expression was 18.6 months (P=0.033). The hazard ratio, adjusted by fitting the Cox proportional hazard models for the mOS in the HGAs with higher IL13Rα2 levels and the HGGs with lower IL13Rα2 levels, was 5.97 (95% CI: 1.76 to 20.32).
CONCLUSIONS: The results showed that IL13Rα2 may be used as a marker of poorer prognosis in HGAs, even among tumors of the same grade.

KEY WORDS: Astrocytoma - Glioma - Glioblastoma - Interleukin-13 receptor alpha2 subunit