Forty-nine Japanese species of Amblyseiinae, a subfamily of Phytoseiidae, are assigned to 10 genera, namely: Neoseiulus Hughes, Typhlodromips De Leon, Typhlodromalus Muma, Amblyseius Berlese, Euseius Wainstein, Proprioseiopsis Muma, Okiseius Ehara, Amblyseiulella Muma, Paraphytoseius Swirski and Shechter, and Indoseiulus Ehara. An annotated list of these species and keys to the genera and species are given, with redescription of Neoseiulus repletus (Wu and Li), recorded from Japan for the first time. Paraphytoseius urumanus (Ehara) differs from P. orientalis (Narayanan, Kaur and Ghai) in that leg IV is provided with five instead of four macrosetae.