Genetika 2013 Volume 45, Issue 3, Pages: 717-726
https://doi.org/10.2298/GENSR1303717O
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Molecular characterization of Prunus mahaleb L. rootstock canditates by ISSR markers
Ozyurt Ibrahim Kursat (Middle Black Sea Transitional Zone Agricultural Research Station, Tokat, Turkey)
Akca Yasar (Gaziosmanpasa University Agricultural Faculty Department of Horticulture, Tokat, Turkey)
Ercisli Sezai (Ataturk University Agricultural Faculty Department of Horticulture, Erzurum, Turkey)
Prunus mahaleb is widely used as rootstocks particularly on calcareous and
dry soils for both sweet and sour cherry cultivars in Turkey. Genetic
diversity and relationships among members of Prunus mahaleb including 29
preselected rootstock candidate accessions from Tokat region in Turkey were
investigated by using 15 ISSR markers. The study revealed high genetic
diversity among accessions, detecting 138 fragments, of which 103 (75%) were
polymorphic. The number of polymorphic bands per primer was between 3-13,
with average of 6.86. The primers 890 and 891 gave the highest polymorphism
ratio (100%). The UPGMA dendrogram and the principal coordinate analysis
revealed a clear differentiation among accessions. Reference rootstock,
SL-64 clustered separately. The study demonstrates that ISSRs provide
promising marker tools in revealing genetic diversity and relationships in
Prunus mahaleb rootstock candidate accessions and can contribute to
efficient identification, conservation, and utilization of germplasm for
rootstock improvement through conventional as well as molecular breeding
approaches.
Keywords: genetic diversity, ISSR, mahaleb, molecular characterization