Archives of Biological Sciences 2011 Volume 63, Issue 3, Pages: 757-762
https://doi.org/10.2298/ABS1103757A
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Direct axillary shoot regeneration from the mature seed explant of the hairy vetch (Vicia villosa Roth)
Aasim Muhammad (Department of Biology, Kamil Ozdag Faculty of Science, Karamanoglu Mehmetbey University, Karaman, Turkey)
Sahin-Demirbag Nurdan (Department of field Crops, Faculty of Agriculture, Ankara University, Diskapi, Ankara, Turkey)
Khawar Mahmood Khalid (Department of field Crops, Faculty of Agriculture, Ankara University, Diskapi, Ankara, Turkey)
Kendir Hayrettin (Department of field Crops, Faculty of Agriculture, Ankara University, Diskapi, Ankara, Turkey)
Özcan Sebahattin (Department of field Crops, Faculty of Agriculture, Ankara University, Diskapi, Ankara, Turkey)
The hairy vetch (Vicia villosa Roth) is a climbing, prostrate or trailing
legume grown as forage.It fixes atmospheric nitrogen, reduces soil erosion
and provides an instant mulch. Multiple axillary shoot regeneration from a
mature seed explant (zygotic embryo with two cotyledons) was obtained on MS
medium containing 0.05 - 1.6 mg/l TDZ with or without 0.10 mg/l IBA. The
frequency (%) of shoot regeneration ranged from 45.83-75.00% with a maximum
number of 28.6 shoots per explant on MS medium containing 0.20 mg/l TDZ-0.10
mg/l IBA. The mean shoot length decreased proportionately with each increase
in TDZ concentration irrespective of the IBA concentration in the culture
medium. However, comparing the two types of regeneration media, longer shoots
were recorded in the presence of IBA in the culture medium. Regenerated
shoots were pulse treated with 50 mg/l IBA for 5, 10 and 20 min for rooting.
Keywords: In vitro, legume, pulse treatment, rooting