doiSerbia

Vegetative compatibility and RFLP analysis of Colletotrichum destructivum isolates from alfalfa and red clover

Vasić Tanja (Institute for Forage Crops, Kruševac)
Krnjaja Vesna (Institute for Animal Husbandry, Belgrade)
Jevremović Darko (Fruit Research Institute, Čačak)
Stanković Slavica (Maize Research Institute "Zemun Polje", Belgrade)
Terzić Dragan (Institute for Forage Crops, Kruševac)
Milenković Jasmina (Institute for Forage Crops, Kruševac)
Marković Jordan (Institute for Forage Crops, Kruševac)

A total of 17 isolates of Colletotrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate nonutilising (nit) mutants were isolated from each of investigated C. destructivum isolates by selecting chlorate-resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self-incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletotrichum species. An identical profile was produced for all C. destructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. destructivum isolates.

Keywords: RFLP, VCGs, M. sativa, T. pratense, C. destructivum

Projekat Ministarstva nauke Republike Srbije, br. TR31057