doiSerbia

Phylogenetic analysis of different Artemisia species based on chloroplast gene rps11

Mahmood Tariq (Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan)
Hassan Nadia (Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan)
Nazar Nazia (Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan)
Naveed Ishrat (Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan)

Artemisia L. of the family Astereacae is a genus with enormous economical and medicinal importance. As a result, the genus Artemisia has been the subject of diversity-focused studies. In the present study, phylogenetic analysis based on restriction fragment length polymorphism of the chloroplast rps11 gene was conducted on eight species of Artemisia that represent the major morphological subgroups. After digestion of the rps11 gene that was amplified from eight species, with six different restriction enzymes, each restriction site was observed and scored on a 12% polyacrylamide gel. The data were analyzed using the Numerical Taxonomy and Multivariate Analysis System to infer the phylogenetic relationship within the genus. A mixed pattern was observed among the species belonging to various taxonomic groups of Artemisia.

Keywords: Artemisia, chloroplast gene, CAPS, phylogenetic analysis