Abstract
Separation and retention of both polar and nonpolar compounds by the same stationary phase can be a useful approach for analyses of complex samples. Biological samples encompass a broad range of polarities and varied chemical properties. A gradient elution run with acetonitrile-water as the mobile phase was used for the separation of polar and non polar compounds from Aphanothece halophytica using ODS C18 column with photo diode array detection. The various fractions collected were identified through Electrospray ionisation-mass spectrometry (ESI-MS) and Nuclear Magnetic Resonance (NMR) analyses. The High Performance Liquid Chromatography (HPLC) method provided good baseline separation of all the compounds in 15 min without the need for sample derivatization. Retrospective review of ESI-MS and NMR data identified the major compounds as nostocyclamide, trimethylglycine, phosphatidyl glycerol.
Keywords: Aphanothece halophytica, bioactive compounds, HPLC, ESI-MS, NMR, nostocyclamide.
The Natural Products Journal
Title:Separation of Bioactive Metabolites from Aphanothece Halophytica Through HPLC and Characterization of the Analytes Through ESI-MS and NMR
Volume: 3 Issue: 2
Author(s): Rashi Vishwakarma and Ashwani Kumar Rai
Affiliation:
Keywords: Aphanothece halophytica, bioactive compounds, HPLC, ESI-MS, NMR, nostocyclamide.
Abstract: Separation and retention of both polar and nonpolar compounds by the same stationary phase can be a useful approach for analyses of complex samples. Biological samples encompass a broad range of polarities and varied chemical properties. A gradient elution run with acetonitrile-water as the mobile phase was used for the separation of polar and non polar compounds from Aphanothece halophytica using ODS C18 column with photo diode array detection. The various fractions collected were identified through Electrospray ionisation-mass spectrometry (ESI-MS) and Nuclear Magnetic Resonance (NMR) analyses. The High Performance Liquid Chromatography (HPLC) method provided good baseline separation of all the compounds in 15 min without the need for sample derivatization. Retrospective review of ESI-MS and NMR data identified the major compounds as nostocyclamide, trimethylglycine, phosphatidyl glycerol.
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Cite this article as:
Vishwakarma Rashi and Rai Ashwani Kumar, Separation of Bioactive Metabolites from Aphanothece Halophytica Through HPLC and Characterization of the Analytes Through ESI-MS and NMR, The Natural Products Journal 2013; 3 (2) . https://dx.doi.org/10.2174/2210315511303020010
DOI https://dx.doi.org/10.2174/2210315511303020010 |
Print ISSN 2210-3155 |
Publisher Name Bentham Science Publisher |
Online ISSN 2210-3163 |
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Recent Advances in Biotransformation of Bioactive Terpenoids using Aspergillus niger
Biotransformation is the reaction catalyzed by biological catalysts of either isolated enzymes or whole cell cultures of microorganisms. This process has been extensively applied in the conversion of bioactive terpenoids with high regio- and stereospecifity. This process has been also applied in the lead discovery and development. Aspergillus niger has ...read more
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