Abstract
In this review, the applications of β galactosidase complementation are described. α Complementation is a naturally occurring process in bacteria and in engineered cells, and can also occur in eukaryotic cells. Two forms of α complementation have been used in high throughput screening (HTS), in which interacting fragments complement with either low or high affinity. Low affinity complementation is used to monitor protein protein interactions, such as those occurring in homodimerization of the epidermal growth factor receptor (EGFR), and provides a robust screen for detection of EGFR inhibitors. High affinity complementation provides the basis for several HTS assays, in which analytes, such as cAMP or IP3, are detected in crude cell lysates. A development of the latter approach is protein labeling, providing for measurement of cell protein expression and trafficking. Collectively, the use of β galactosidase complementation provides a novel and flexible technology for highly sensitive, homogeneous HTS assay development.
Keywords: enzyme fragment complementation, galactosidase, homogenous hts assays, protein:protein interactions, protein expression, g protein coupled receptors, proteases, receptor kinases
Combinatorial Chemistry & High Throughput Screening
Title: β Galactosidase Enzyme Fragment Complementation as A Novel Technology for High Throughput Screening
Volume: 6 Issue: 4
Author(s): Richard M. Eglen and Rajendra Singh
Affiliation:
Keywords: enzyme fragment complementation, galactosidase, homogenous hts assays, protein:protein interactions, protein expression, g protein coupled receptors, proteases, receptor kinases
Abstract: In this review, the applications of β galactosidase complementation are described. α Complementation is a naturally occurring process in bacteria and in engineered cells, and can also occur in eukaryotic cells. Two forms of α complementation have been used in high throughput screening (HTS), in which interacting fragments complement with either low or high affinity. Low affinity complementation is used to monitor protein protein interactions, such as those occurring in homodimerization of the epidermal growth factor receptor (EGFR), and provides a robust screen for detection of EGFR inhibitors. High affinity complementation provides the basis for several HTS assays, in which analytes, such as cAMP or IP3, are detected in crude cell lysates. A development of the latter approach is protein labeling, providing for measurement of cell protein expression and trafficking. Collectively, the use of β galactosidase complementation provides a novel and flexible technology for highly sensitive, homogeneous HTS assay development.
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Cite this article as:
Eglen M. Richard and Singh Rajendra, β Galactosidase Enzyme Fragment Complementation as A Novel Technology for High Throughput Screening, Combinatorial Chemistry & High Throughput Screening 2003; 6 (4) . https://dx.doi.org/10.2174/138620703106298473
DOI https://dx.doi.org/10.2174/138620703106298473 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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