Stationary Phase Expression, Purification, and Characterization of XorKI, a Restriction Endonuclease from Xanthomonas oryzae pv. oryzae

Title: Stationary Phase Expression, Purification, and Characterization of XorKI, a Restriction Endonuclease from Xanthomonas oryzae pv. oryzae

Volume: 17 Issue: 3

Author(s): Woo Young Kang and Young Kee Chae

Affiliation:

Keywords: XorKI, restriction endonuclease, Xanthomonas, stationary state induction

Abstract: An endonuclease from Xanthomonas oryzae pathovar oryzae KACC 10331, XorKI, was heterologously produced in Escherichia coli by applying the stationary state induction method. The yield was 5.4 mg of XorKI per liter of LB medium. XorKI existed in multiple oligomeric forms as evidenced by gel filtration chromatography. The specific activity of purified XorKI was 323000 units per mg.

Cite this article as:

Kang Young Woo and Chae Kee Young, Stationary Phase Expression, Purification, and Characterization of XorKI, a Restriction Endonuclease from Xanthomonas oryzae pv. oryzae, Protein & Peptide Letters 2010; 17 (3) . https://dx.doi.org/10.2174/092986610790780378