The preparation of 3,5-dihydroxy-4-isopropylstilbene nanoemulsion and in vitro release

Yue Zhang^1,2, Jungang Gao¹, Hetang Zheng², Ran Zhang³, Yucui Han^2
1College of Chemistry and Enviromental Science, Hebei University, Baoding, China; ²School of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, China; ³College of Chemical Engineering, East China University of Science and Technology, Shanghai, China

Abstract: We have reported a novel procedure to prepare 3,5-dihydroxy-4-isopropylstilbene (DHPS) nanoemulsion, using a low-energy emulsification method. Based on the phase diagram, the optimum prescription of nanoemulsion preparation was screened. With polyoxyethylenated castor oil (EL-40) as the surfactant, ethanol as the co-surfactant, and isopropyl myristate (IPM) as the oil phase, the DHPS nanoemulsion was obtained with a transparent appearance, little viscosity, and spherically uniform distribution verified by transmission electron microscopy and laser scattering analyzer. The nanoemulsion was also determined by FT-Raman spectroscopy. The DHPS nanoemulsion demonstrated good stability and stable physical and chemical properties. The nanoemulsion dramatically improved the transdermal release of DHPS (from 8.02 µg · cm^-2 to 273.15 µg · cm^-2) and could become a favorable new dosage form for DHPS.

Keywords: nanoemulsion, 3,5-dihydroxy-4-isopropylstilbene, DHPS, pseudo-ternary phase diagram