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Electrochemical Control of Bioluminescence by Blocking the Adsorption of the Bacterial Luciferase Using a Mercaptobipyridine Self-assembled Monolayer

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Abstract

An N-butyl-N'-(4-mercaptobutyl)-4,4′-bipyridinium (4BMBP) was modified on a gold electrode to improve the electrochemical control of the bacterial luciferase (BL) luminescence system. The 4BMBP-modified gold electrode (4BMBP/Au) was able to prevent the adsorption of BL on the electrode surface, and enhanced the electrochemical regeneration rate of the reduced flavin mononucleotide (FMNH2), which is one of the substrates of the BL luminescence reaction. By using the 4BMBP/Au, the luminescence intensity increased by about 27% compared to that of a bare gold electrode (bare Au). Moreover, the modified electrode improved the time required for analysis because the modified layer prevented BL adsorption. Even without a refreshing procedure for each measurement, a constant luminescence intensity could be observed, and the analysis time was reduced to half (about 10 min) for one sample. The 4BMBP/Au is not only useful to control of the BL luminescence system, but also for electrochemical measurements in the presence of proteins.

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Authors and Affiliations

  1. Faculty of Pure and Applied Sciences and Center for Research in Isotopes and Environmental Dynamics, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan

    Shinya Yamasaki

  2. Department of Applied Chemistry, School of Applied Sciences National Defense Academy, 1-10-20 Hashirimizu, Yokosuka, 239-8686, Japan

    Shuto Yamada

  3. Department of Chemical and Biological Science, Faculty of Science, Japan Women’s University, 2-8-1 Mejirodai, Bunkyo, Tokyo, 112-8681, Japan

    Hiroyuki Takemura

  4. Department of Chemistry, Kyushu University, 744 Motooka, Nishi, Fukuoka, 819-0395, Japan

    Kô Takehara

Authors
  1. Shinya Yamasaki
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  2. Shuto Yamada
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  3. Hiroyuki Takemura
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  4. Kô Takehara
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Correspondence to Shinya Yamasaki.

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Yamasaki, S., Yamada, S., Takemura, H. et al. Electrochemical Control of Bioluminescence by Blocking the Adsorption of the Bacterial Luciferase Using a Mercaptobipyridine Self-assembled Monolayer. ANAL. SCI. 33, 307–311 (2017). https://doi.org/10.2116/analsci.33.307

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  • DOI: https://doi.org/10.2116/analsci.33.307

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