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The Use of an Enzyme-based Sensor Array to Fingerprint Proteomic Signatures of Sera from Different Mammalian Species

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Abstract

A cross-reactive sensor array consisting of polyion complexes (PICs) between anionic enzymes and poly(ethylene glycol)- modified (PEGylated) polyamines has been used to identify the source of mammalian sera. Although the catalytic activity of enzymes was inhibited by PIC formation with PEGylated polyamines, the subsequent addition of sera caused enzyme releases from PICs through competitive interactions between PICs and serum proteins, generating unique response patterns of changes in the enzyme activity. Linear discriminant analysis of the obtained patterns enabled the discrimination of five sera from different mammalian sources.

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Acknowledgements

This research was supported in part by a Grant in Aid for Scientific Research (No. 21256001) from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and by a grant from the Environmental Research and Technology Development Fund (No.5-1306) of the Ministry of the Environment, Japan.

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Authors and Affiliations

  1. Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan

    Shunsuke Tomita, Ryoji Kurita & Osamu Niwa

  2. Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo, 153-8902, Japan

    Saki Yokoyama & Keitaro Yoshimoto

Authors
  1. Shunsuke Tomita
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  2. Saki Yokoyama
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  3. Ryoji Kurita
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  4. Osamu Niwa
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  5. Keitaro Yoshimoto
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Correspondence to Shunsuke Tomita or Keitaro Yoshimoto.

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Tomita, S., Yokoyama, S., Kurita, R. et al. The Use of an Enzyme-based Sensor Array to Fingerprint Proteomic Signatures of Sera from Different Mammalian Species. ANAL. SCI. 32, 237–240 (2016). https://doi.org/10.2116/analsci.32.237

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  • DOI: https://doi.org/10.2116/analsci.32.237

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