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Structural Transition of Bombyx mori Liquid Silk Studied with Vibrational Circular Dichroism Spectroscopy

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Abstract

We investigated the structural transition from liquid silk to silk fibers with vibrational circular dichroism spectroscopy. Liquid silk showed a major right-handed optically active band at around 1650 cm−1 and a minor one at around 1680 cm−1. The former disappeared over time, while the intensity in the latter increased. With the former wavenumber, liquid silk mainly adopted a random-coil structure. In contrast, the latter may reflect an intermediate structure in the transition. Furthermore, two right-handed bands at around 1630 and 1660 −1 appeared with the disappearance of the major band, and then the wavenumber of the former shifted to around 1620 −1. The shift results from the decrease in the frequency of the CO stretching mode due to the stacking of the β-sheet that comprises fibers. The band at 1660 −1 may reflect another intermediate structure due to its strong correlation with that at 1620 −1 in terms of their temporal change in intensity.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 162-8601, Japan

    Toshinori Morisaku, Sho Arai, Kohzo Konno & Hiroharu Yui

  2. Tenure-Track Program for Innovative Research, University of Fukui, 3-9-1 Bunkyo, Fukui, Fukui, 910-8507, Japan

    Yu Suzuki

  3. Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo, 184-8588, Japan

    Tetsuo Asakura

Authors
  1. Toshinori Morisaku
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  2. Sho Arai
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  3. Kohzo Konno
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  4. Yu Suzuki
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  5. Tetsuo Asakura
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  6. Hiroharu Yui
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Correspondence to Hiroharu Yui.

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Morisaku, T., Arai, S., Konno, K. et al. Structural Transition of Bombyx mori Liquid Silk Studied with Vibrational Circular Dichroism Spectroscopy. ANAL. SCI. 31, 763–768 (2015). https://doi.org/10.2116/analsci.31.763

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  • DOI: https://doi.org/10.2116/analsci.31.763

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