Abstract
An ultra-sensitive colorimetric method to quantitate hundreds of polynucleotide molecules by gold nanoparticles with silver enhancement has been developed. The hybridization products from the target polynucleotides with biotin-labeled probes and gold nanoparticle-functioned oligonucleotides were immobilized to microplates via avidin–biotin system, and the absorbance signals of gold nanoparticles were amplified by silver enhance solution. This sandwich colorimetric assay can detect as few as 600 molecules for single-strand oligonucleotides and as few as 6000 molecules for double-strand polynucleotides in a 50 μL reaction system.
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Li, BS., Zhao, LF., Zhang, C. et al. Ultra-sensitive Colorimetric Method to Quantitate Hundreds of Polynucleotide Molecules by Gold Nanoparticles with Silver Enhancement. ANAL. SCI. 22, 1367–1370 (2006). https://doi.org/10.2116/analsci.22.1367
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DOI: https://doi.org/10.2116/analsci.22.1367