Abstract
An amperometric immunosensor was constructed by dispersing graphite, schistosoma-japonicum antigen (SjAg) and silica sol-gel at low temperature. The performance characteristics of the prepared immunosensor were examined in the buffered solution of o-aminophenol (o-AP) used as a substrate. It exhibited excellent physical and electrochemical stability with a renewable external surface. A competitive binding assay was employed to determine schistosoma-japonicum antibody (SjAb) with the aid of horseradish peroxidase labeled SjAb (HRP-SjAb). The experimental parameters for SjAb assay were optimized, including the amount of labeled SjAb in incubation solution, incubation time, temperature and the pH of solution. The use of o-AP substrate and amperometric detection at -250 mV (vs. SCE) results in a determination limit of 0.32 μg/ml and a linear range extending up to 0.18 μg/ml. The results of SjAb assay in serum samples demonstrate the feasibility of using the proposed immunosensor for clinical analysis.
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Zhong, TS., Liu, G. Silica Sol-gel Amperometric Immunosensor for Schistosoma Japonicum Antibody Assay. ANAL. SCI. 20, 537–541 (2004). https://doi.org/10.2116/analsci.20.537
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DOI: https://doi.org/10.2116/analsci.20.537