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Analysis of Nitrogen-containing Compounds in Mouth-exhaled Breath by Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry

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Abstract

Nitrogen-containing compounds are important components in human breath. However, their origins have not yet been clearly understood. In this study, a modified electrospray ionization (ESI) source coupling with quadrupole time-of-flight mass spectrometry has been used for breath analysis. Fourteen nitrogen-containing compounds were identified in mouth-exhaled breath, and 10 of them were from the oral cavity and oropharynx. Moreover, 8 of these nitrogen-containing compounds were recognized as endogenous metabolites. This result provides important clues for exploring the biological origins of these nitrogen-containing compounds. Observation of the ion suppression phenomenon also indicates that breath analysis should be carried out after clearing of the oral cavity and oropharynx, or directly through nose-breathing to eliminate the influence of those nitrogen-containing compounds from the oral cavity.

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Acknowledgments

This study was funded by the Key Program of 13th five-year plan, CASHIPS (No. KP-2017-25), Provincial Natural Foundation of Anhui Province (No. 1808085MF169) and the Natural Science Research Project of Anhui Universities (No. KJ2018A0086).

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Authors and Affiliations

  1. Anhui Province Key Laboratory of Medical Physics and Technology, Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, 230031, P. R. China

    Wenzhao Zhou, Xue Zou, Lei Xia, Yan Lu, Chengyin Shen, Chaoqun Huang & Yannan Chu

  2. School of Electrical and Information Engineering, Anhui University of Science and Technology, Huainan, Anhui, 232001, P. R. China

    Yan Hong

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  1. Wenzhao Zhou
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  2. Yan Hong
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  3. Xue Zou
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Correspondence to Yannan Chu.

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Zhou, W., Hong, Y., Zou, X. et al. Analysis of Nitrogen-containing Compounds in Mouth-exhaled Breath by Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry. ANAL. SCI. 35, 1155–1159 (2019). https://doi.org/10.2116/analsci.19N018

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  • DOI: https://doi.org/10.2116/analsci.19N018

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