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Optical Sensing Platform for the Colorimetric Determination of Silver Nanoprisms and Its Application for Hydrogen Peroxide and Glucose Detections Using a Mobile Device Camera

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Abstract

Silver nanoprisms (AgNPrs) have a unique localized surface plasmon resonance, resulting in strong absorption and scattering within the visible light region. In this work, we propose image acquisition from colloidal solutions of AgNPrs using a combination of transmitted and scattered light. The developed measurement technique could be carried out by separately recording transmitted and scattering images of the solutions, using a mobile device camera prior to a calculation of the empirical absorption value (IA). The IA value of green for AgNPrs solutions was found to be in agreement with the absorption spectra obtained using a conventional spectroscopic technique. This technique was utilized for the quantifications of hydrogen peroxide and glucose. Good linearities between ∆IA and those typical analytes were observed. The limit of detection for the typical biosensor of glucose was 19.8 µM. As such, we expect the methodology herein developed for hydrogen peroxide and glucose determinations by means of monitoring the color change of transmitted and scatting images from solutions to contribute to the development of simple, rapid, and reliable detection systems to be further applied to biochemical analysis and clinical diagnosis, as well as to household biosensor applications.

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Acknowledgments

This work was supported by JSPS KAKENHI Grant Number JP16K13662 and JP17H03231, and Niigata University U-go grant.

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Correspondence to Chutiparn Lertvachirapaiboon or Akira Baba.

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Lertvachirapaiboon, C., Maruyama, T., Baba, A. et al. Optical Sensing Platform for the Colorimetric Determination of Silver Nanoprisms and Its Application for Hydrogen Peroxide and Glucose Detections Using a Mobile Device Camera. ANAL. SCI. 35, 271–276 (2019). https://doi.org/10.2116/analsci.18P412

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  • DOI: https://doi.org/10.2116/analsci.18P412

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