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Amperometric Glucose Biosensor Utilizing Zinc Oxide-chitosan-glucose Oxidase Hybrid Composite Films on Electrodeposited Pt-Fe(III)

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Abstract

We developed an amperometric glucose biosensor based on glucose oxidase (GOx) embedded in zinc oxide (ZnO)- chitosan (CS) hybrid composite films on electrodeposited Pt-Fe(III). This sensor exhibited a fast amperometric response (less than 10 s) to glucose, linearity from 10 μM to 11.0 mM of glucose with a detection limit of 1.0 μM (S/N = 3) and sensitivity of 30.70 μA mM 1 cm−2. An apparent Michaelis-Menten constant of 5.19 mM indicated high affinity between glucose and GOx immobilized in the ZnO-CS films. The effect of interferences such as uric acid, ascorbic acid, and acetaminophen on the performance of this sensor was negligible. In addition, this sensor retained 87% of its initial performance after two weeks of storage at 4°C, indicating that the hybrid composite films allowed successful immobilization of GOx with its high enzymatic activity.

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Acknowledgments

This work was supported by the National Research Foundation of Korea (2016R1C1B2011912) for Y.-R. K.

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Authors and Affiliations

  1. Department of Chemistry Education, Seoul National University, Seoul, 08826, Korea

    Eun-Sook Paik & Hun-Gi Hong

  2. Department of Chemistry, Kwangwoon University, Seoul, 01897, Korea

    Yang-Rae Kim

Authors
  1. Eun-Sook Paik
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  2. Yang-Rae Kim
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  3. Hun-Gi Hong
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Correspondence to Yang-Rae Kim or Hun-Gi Hong.

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Paik, ES., Kim, YR. & Hong, HG. Amperometric Glucose Biosensor Utilizing Zinc Oxide-chitosan-glucose Oxidase Hybrid Composite Films on Electrodeposited Pt-Fe(III). ANAL. SCI. 34, 1271–1276 (2018). https://doi.org/10.2116/analsci.18P054

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