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Determination of Anthracene in Water Samples by ELISA Using AhR Binding

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Abstract

The degradation of polycyclic aromatic hydrocarbons (PAHs) can generate AhR-binding compounds, exhibiting genotoxicity and carcinogenicity. In this investigation, aryl hydrocarbon receptor (AhR) from carp and anthracene (Ant) were coupled as antigen to establish an indirect competition ELISA (ic-ELISA) with an AhR-Ant antibody. A standard curve was determined for the ic-ELISA concerning detection range and limit. Also, the specificity, stability and the recovery of the ic-ELISA were checked. Results indicate that the ratio of antibody to antigen titer is 1:64000. The resulting standard curve is Y = 21.326 × X + 1.8213. The detection range lies within 10-1000 ng mL−1 and the limiting concentration is 2.43 ng mL−1. The cross reaction ratio (CR) between Ant and naphthalene (Nap), Ant and phenanthrene (Phe) or Ant and fluoranthene (Flu) were 5.7, 19.1 and less than 0.1%, respectively. The range of the coefficient of variance (C.V) amounts was from 4.2 up to 9.5% and the recovery range was from 90 to 115%. These results show that the AhR-Ant ic-ELISA is sensitive, and can be used as a technical support to quantify Ant in the environment.

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Acknowledgments

The authors are thankful for the financial support of the Shanghai Natural Science Fund (15ZR1401500), China.

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Authors and Affiliations

  1. College of Environmental Science and Engineering, Donghua University, Ren Min North Road 2999, Songjiang District, 201620, China, Shanghai

    Yanfeng Lv, Xiaoxiang Zhao, Lian Yang, Xian Zhang & Yujie Bai

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  1. Yanfeng Lv
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  2. Xiaoxiang Zhao
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  3. Lian Yang
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  4. Xian Zhang
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  5. Yujie Bai
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Correspondence to Xiaoxiang Zhao.

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Lv, Y., Zhao, X., Yang, L. et al. Determination of Anthracene in Water Samples by ELISA Using AhR Binding. ANAL. SCI. 34, 421–426 (2018). https://doi.org/10.2116/analsci.17P389

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