Abstract
The fluorescence spectral characteristics and the interaction of several water-soluble metal complexes of Schiff-base with DNA are described. Among the complexes tested, Mn-Schiff-base bound to DNA showed a marked decrease in the fluorescence intensity with a blue shift of the excitation and emission peaks. Some hypochromism in the UV absorption spectra was also observed. KI quenching and competitive binding to DNA between Mn-Schiff-base and ethidium bromide (EB) were studied in connection with other experimental observations to show that the interactive model between Mn-Schiff-base and DNA is an intercalative one. The pH and salt effect on the fluorescence properties was also investigated. The linear relationship between F/F0 and the concentration of calf thymus DNA covers 3.0 × 10–6–2 × 10–4 mol L–1, which can be utilized for determining traces of calf thymus DNA with a detection limit of 8.0 × 10–7 mol L–1 in base pairs.
Similar content being viewed by others
References
M. AI-Hakeem and S. S. Sommer, Anal. Biochem., 1987, 163, 433.
J. B. Le Pecq and C. Paoletti, Anal. Biochem., 1966, 17, 100.
M.D. Topal and J. R. Fresco, Biochemistry, 1980, 19, 5531.
G. P. Dutta, Histochemie, 1970, 24, 29.
C. V. Kvmar and H. Emma, J. Chem. Soc., Chem. Commun., 1992, 470.
D. Z. Yang, J. T. Strode, H. P. Spielmann, A. J. Wang, and T. G. Burake, J. Am. Chem. Soc., 1998, 120, 2979.
H. E. Moser and P. B. Dervan, Science, 1987, 238, 645.
J.-C. Francois, T. Saison-Behmoaras, M. Chassignol, N. T. Thuong, and C. Helene, J. Biol. Chem., 1989, 264, 5891.
R. S. Youngquist and P. B. Dervan, J. Am. Chem. Soc., 1985, 107, 5528.
W. S. Wade and P. B. Dervan, J. Am. Chem. Soc., 1987, 109, 1574.
D. S. Sigman, A. Mazumder, and D. M. Perrin, Chem. Rev., 1993, 93, 2295.
E. M. Hodnett and W. J. Dunn, J. Med. Chem., 1970, 13, 768.
D. Kessel, A. F. Al-Sayyab, E. M. H. Jaffar, and A. H. H. Al-Lami, Iraqi J. Sci., 1981, 22, 312.
D. Y. Zhao, D. W. Pang, Z. L. Wang, J. K. Cheng, Z. F. Luo, C. J. Feng, H. Y. Sheng, and X. C. Zhang, Acta Chim. Sinica (in Chinese), 1997, 56, 178.
G. W. Yang, X. P. Xia, H. Tu, and C. X. Zhao, Chinese J. App. Chem. (in Chinese), 1995, 12(2), 13.
G. W. Yang, X. P. Xia, H. Tu, and C. X. Zhao, Chem. Res. Appl. (in Chinese), 1995, 7(1), 41.
E. M. Hodnett and P. D. Mooney, J. Med. Chem., 1970, 13, 786.
E. M. Hodnett and W. J. Dunn, J. Med. Chem., 1972, 15, 339.
J. R. Morrow and K. Kolasa, Inorg. Chim. Acta, 1992, 195, 245.
K. Sato, M. Chikira, Y. Fujii, and A. Komatsu, J. Chem. Soc., Chem. Commun., 1994, 625.
S. Bhattacharya and S. S. Mandal, J. Chem. Soc., Chem. Commun., 1995, 2489.
S. Routier, J. L. Bernier, M. J. Waring, P. Coloson, C. Houssier, and C. Bailly, J. Org. Chem., 1996, 61, 2326.
G. J. D. Griffin and H. John, J. Org. Chem., 1996, 35, 4837.
G. D. Liu, X. Yang, Z. P. Chen, G. L. Shen, and R. Q. Yu, Anal. Sci., in press.
S. J. Angyal, P. J. Morris, J. R. Tetaz, and J. G. Wilson, J. Chem. Soc., 1950, 2141.
M. J. Warning, J. Mol. Biol., 1965, 13, 269.
J. B. Lepecq and C. Paoletti, J. Mol. Biol., 1967, 27, 87.
Z. L. Li, J. H. Chen, M. L. Li, and R. Q. Yu, Sci. China, Ser. B (in Chinese), 1991, 11, 1193.
H. Sigel, Chem. Soc. Rev., 1993, 255.
G. Z. Chen, X. Z. Huang, Z. Z. Xu, and Z. B. Wang, “The Fluorescence Analysis”, 2nd ed., 1990, Silica Publish, Beijin.
G. Scatchard, Ann. N. Y. Acad. Sci., 1949, 51, 660 and references therein.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Liu, GD., Liao, JP., Huang, SS. et al. Fluorescence Spectral Study of Interaction of Water-soluble Metal Complexes of Schiff-base and DNA. ANAL. SCI. 17, 1031–1036 (2001). https://doi.org/10.2116/analsci.17.1031
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2116/analsci.17.1031