Characterization and Potent Application of Pleurotus floridanus Trypsin Inhibitor (PfTI)

Manzur Ali Pannippara; Sapna Kesav; Rekha Mol Kollakal Naduvil Raghavan; Abraham Mathew; Sarita Ganapathy Bhat; Elyas Kothanan Kozhiyil

doi:10.20307/nps.2020.26.3.207

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한국생약학회
Natural Product Sciences 학술저널
Natural Product Sciences Vol.26 No.3
2020.9 207 - 213 (7page)
DOI : 10.20307/nps.2020.26.3.207

저자정보

Manzur Ali Pannippara (Cochin University of Science and Technology)
Sapna Kesav (Cochin University of Science and Technology)
Rekha Mol Kollakal Naduvil Raghavan (Cochin University of Science and Technology)
Abraham Mathew (Cochin University of Science and Technology)
Sarita Ganapathy Bhat (Cochin University of Science and Technology)

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Characterization and in vitro inhibition studies of protease inhibitor from the mushroom Pleurotus floridanus (PfTI) towards the pest Papilio demoleus is studied. The addition of 1 mM Mn²⁺, Na²⁺, Ba²⁺ and Ni ²⁺ enhanced the PfTI activity. The ICP-atomic emission spectrum showed the presence of Ca²⁺, Mg²⁺ and Zn²⁺ in the PfTI. Surfactants SDS and CTAB at a concentration of 1% reduced the PfTI activity whereas, the nonionic detergents Triton X and Tween 80 increased the activity. The inhibitory activity gradually decreased with increase in concentration of DMSO and H₂O₂. The activity was increased by dithiothreitol up to a concentration of 80 μM and inactivated at 140 μM. The activity of PMSF modified PfTI was drastically reduced to 0.234 U/mL at 4 mM concentration and similar results were obtained for modification of cysteine by N-Ethylmaleimide at slightly higher concentrations. The complex of trypsin and PfTI showed complete loss in fluorescence intensity at 343 nm compared with control. In vitro inhibition studies of PfTI with midgut proteases isolated from citrus pest P. demoleus with protease activity of 1.236 U was decreased to 0.613 U by 50 μL (0.1 mg/mL) of the inhibitor. Inhibitor was stable up to 0.04 M concentration of HCl.

#Trypsin inhibitor #Pleurotus floridanus #Characterization