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中医杂志(英文版). 2021, (03)

Efficacy of Silene arenosa extract on acetylcholinesterase in Bungarus sindanus（krait） venomEnglish Full Text

Adil Shah;Mushtaq Ahmed;Naila Sher;Nadia Mushtaq;Rahmat Ali Khan;Fozia;Midrarullah;Department of Biotechnology, Faculty of Biological Sciences, University of Science ＆ Technology;Department of Botany, Faculty of Biological Sciences, University of Science ＆ Technology;Department of Biochemistry, KMU Institute of Medical Sciences;Department of Biotechnology, Saheed Benazir Bhutto University;

Abstract: OBJECTIVE:To examine the efficacy of Silene arenosa extract on acetylcholinesterase （AChE） of krait （Bungarus Sindanus） snake venom.METHODS:The present project designed to evaluate the inhibition of AChE by following standard procedures.RESULTS:Statistical analysis of the results showed that Silene arenosa exerted 73%inhibition against the krait venom acetylcholinesterase at fixed substrate acetylcholine （ACh） concentration （0.5 mM）.Kinetic analysis using the Lineweaver Burk plot revealed that Silene arenosa caused a competitive type of inhibition i.e.Km values increased from 26.6 to 93.3 mM （26.6%to 93.3%） and Vmaxremained constant in a concentration-dependent manner.Silene arenosa competes with the substrate to bind at the active site of the enzyme.The Kmappof venom AChE for Silene arenosa increased from 60%to 81.6%and the Vmaxappremains constant.Ki(inhibition constant was estimated to be 48μg for snake venom;while the Km（Michaelis-Menten constant of AChE-substrate into AChE and product） was estimated to be 0.5 mM.The IC50of AchE calculated for Silene arenosa was 67μg.CONCLUSION:The present results suggest that Silene arenosa extract can be considered as an inhibitor of snake venom AChE.
Keywords：

acetylcholinesterase; snake venoms; silene arenosa; competitive inhibition;

  • DOI：

    10.19852/j.cnki.jtcm.2021.03.001

  • Series：

    (E) Medicine ＆ Public Health

  • Subject：

    Traditional Chinese Medicinal Herbs

  • Classification Code：

    R285

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